Pd-L1/B7-H1 ELISA Kit for Human

Programmed death-ligand 1 (PD-L1) is a typ1 transmembrane glycoprotein also known as CD274 and was first characterized as an immune regulatory molecule, B7-H1. The PD-1 receptor is found on activated B cells, T cells, and myeloid cells and modulates activation and inhibition. The binding of PD-L1 to the PD-1 receptor on T cells delivers a signal that inhibits the TCR-mediated activation of IL-2 production and T cell proliferation. Research has determined that the upregulation of PD-L1 may allow cancers to evade the host immune system.
Human PD-L1/B7-H1 Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid phase immunoassay specially designed to measure Human PD-L1/B7-H1 with a 96-well strip plate that is pre-coated with mouse monoclonal antibody specific for B7-H1/PD-L1. It contains NS0-expressed recombinant Human PD-L1/B7-H1. The detection antibody is a biotinylated goat polyclonal antibody specific for PD-L1/B7-H1. The kit is analytically validated with ready to use reagents.

Small volumes of PD-L1/B7-H1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS697424 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the PD-L1/B7-H1, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing PD-L1/B7-H1. The ELISA analytical biochemical technique of the MBS697424 kit is based on PD-L1/B7-H1 antibody-PD-L1/B7-H1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect PD-L1/B7-H1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, PD-L1/B7-H1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).