Thrombopoietin (THPO) ELISA Kit for Human

Name: Human THPO ELISA Kit; Thrombopoietin; MyBioSource
Brand: MyBioSource
SKU: MBS700105
Price: 295 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS700105

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for TPO has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any TPO present is bound by the… More details

$295 / 24-Strip-Wells (LIMIT 1) +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

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Product Overview

Online Datasheet in PDF

Product Name

thrombopoietin (THPO), ELISA Kit

Full Product Name

Human Thrombopoietin, TPO ELISA Kit

Product Gene Name

THPO elisa kit

Product Synonym Names

Human Thrombopoietin; TPO ELISA kit; MGC163194; MGDF; MKCSF; ML; MPLLG; TPO; MPL ligand; c-mpl ligand; megakaryocyte colony-stimulating factor; megakaryocyte growth and development factor; megakaryocyte stimulating factor; myeloproliferativ; thrombopoietin

Species Reactivity

Human

Assay Type

Sandwich (Quantitative)

Samples

Serum, plasma.

Detection Range

6.25 pg/ml-400 pg/ml.

Sensitivity

1.56 pg/ml.

Specificity

This assay has high sensitivity and excellent specificity for detection of human TPO. No significant cross-reactivity or interference between human TPO and analogues was observed.

Preparation and Storage

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Inter-assay Precision

Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

Intra-assay Precision

Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.

Molecular Weight

37,823 Da

OMIM

600044

3D Structure

ModBase 3D Structure for P40225

ISO Certification

Manufactured in an ISO 9001:2015 Certified Laboratory.

Other Notes

Small volumes of THPO elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for THPO purchase

MBS700105 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the thrombopoietin (THPO) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing THPO. The ELISA analytical biochemical technique of the MBS700105 kit is based on THPO antibody-THPO antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect THPO antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, THPO. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for THPO. It may not necessarily be applicable to this product.

NCBI GI #

4507493

NCBI GeneID

7066

NCBI Accession #

NP_000451.1

UniProt Primary Accession #

P40225

UniProt Secondary Accession #

Q13020; Q15790; Q15791; Q15792; A1L3Y0; B7ZLR8; B9EGA8

UniProt Related Accession #

P40225

GenBank Nucleotide #

NM_000460.3

NCBI Official Full Name

thrombopoietin isoform 1

NCBI Official Synonym Full Names

thrombopoietin

NCBI Official Symbol

THPO

NCBI Official Synonym Symbols

ML; TPO; MGDF; MKCSF; MPLLG; THCYT1

UniProt Protein Name

Thrombopoietin

UniProt Synonym Protein Names

C-mpl ligand; ML; Megakaryocyte colony-stimulating factor; Megakaryocyte growth and development factor; MGDF; Myeloproliferative leukemia virus oncogene ligand

Protein Family

Thrombopoietin

UniProt Gene Name

THPO

UniProt Synonym Gene Names

MGDF; ML; MGDF

UniProt Entry Name

TPO_HUMAN

NCBI Protein Information

thrombopoietin; MPL ligand; c-mpl ligand; prepro-thrombopoietin; megakaryocyte stimulating factor; megakaryocyte colony-stimulating factor; megakaryocyte growth and development factor; myeloproliferative leukemia virus oncogene ligand

NCBI Summary for THPO

Megakaryocytopoiesis is the cellular development process that leads to platelet production. The main functional protein encoded by this gene is a humoral growth factor that is necessary for megakaryocyte proliferation and maturation, as well as for thrombopoiesis. This protein is the ligand for MLP/C_MPL, the product of myeloproliferative leukemia virus oncogene. Mutations in this gene are the cause of thrombocythemia 1. Alternative promoter usage and differential splicing result in multiple transcript variants differing in the 5' UTR and/or coding region. Multiple AUG codons upstream of the main open reading frame (ORF) have been identified, and these upstream AUGs inhibit translation of the main ORF at different extent. [provided by RefSeq, Feb 2014]

UniProt Comments for THPO

Function: Lineage-specific cytokine affecting the proliferation and maturation of megakaryocytes from their committed progenitor cells. It acts at a late stage of megakaryocyte development. It may be the major physiological regulator of circulating platelets.

Research Articles on THPO

1. An Arg->Cys substitution at residue 38 or residue 17 excluding the 21-AA signal peptide of the receptor binding domain was found in a family with aplastic anemia. Adding a 5th cysteine may disrupt normal disulfide bonding & receptor binding.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with THPO elisa kit

Reference Product	PubMed Publications
MPL elisa kit	>23 publications with THPO and MPL
KITLG elisa kit	>8 publications with THPO and KITLG
SELP elisa kit	>1 publications with THPO and SELP

Pathways associated with THPO elisa kit

Products by Pathway	Pathway Diagram
Hematopoietic Cell Lineage Pathway antibodies	Hematopoietic Cell Lineage Pathway Diagram
Hematopoietic Cell Lineage Pathway antibodies	Hematopoietic Cell Lineage Pathway Diagram
Hemostasis Pathway antibodies	Hemostasis Pathway Diagram
Platelet Aggregation (Plug Formation) Pathway antibodies	Platelet Aggregation (Plug Formation) Pathway Diagram
Platelet Activation, Signaling And Aggregation Pathway antibodies	Platelet Activation, Signaling And Aggregation Pathway Diagram

Diseases associated with THPO elisa kit

Disease Name	Pubmed Publications
Thrombocytosis Antibodies	>9 publications with THPO and Thrombocytosis
Anemia Antibodies	>5 publications with THPO and Anemia
Cardiovascular Diseases Antibodies	>5 publications with THPO and Cardiovascular Diseases
Lung Neoplasms Antibodies	>3 publications with THPO and Lung Neoplasms
Congenital Abnormalities Antibodies	>2 publications with THPO and Congenital Abnormalities
Carcinoma, Squamous Cell Antibodies	>2 publications with THPO and Carcinoma, Squamous Cell
Pain Antibodies	>2 publications with THPO and Pain
Thrombocythemia, Essential Antibodies	>2 publications with THPO and Thrombocythemia, Essential
Precancerous Conditions Antibodies	>1 publications with THPO and Precancerous Conditions
Hypertension Antibodies	>1 publications with THPO and Hypertension