Nuclear Receptor Subfamily 3, Group C, Member 1 (Glucocorticoid Receptor) (NR3C1) ELISA Kit for Rat

Name: Rat NR3C1 ELISA Kit; MyBioSource
Brand: MyBioSource
SKU: MBS700327
Price: 295 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS700327

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for GR has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any GR present is bound by the immobilized… More details

$295 / 24-Strip-Wells (LIMIT 1) +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Request Current Manual

Product Overview

Online Datasheet in PDF

Product Name

nuclear receptor subfamily 3, group C, member 1 (glucocorticoid receptor) (NR3C1), ELISA Kit

Full Product Name

Rat glucocorticoid receptor, GR ELISA Kit

Product Gene Name

NR3C1 elisa kit

Product Synonym Names

Rat glucocorticoid receptor; GR ELISA Kit; GCCR; GCR; GR; GRL; glucocorticoid receptor; nuclear receptor subfamily 3; group C; member 1 (glucocorticoid receptor)

Species Reactivity

Rat

Assay Type

Sandwich

Samples

Serum, plasma, tissue homogenates, Cell lysates

Detection Range

15.6 pg/ml-1000 pg/ml

Sensitivity

3.9 pg/ml

Specificity

This assay has high sensitivity and excellent specificity for detection of Rat NR3C1. No significant cross-reactivity or interference between Rat NR3C1 and analogues was observed.

Preparation and Storage

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Intra-assay Precision

Intra-assay Precision (Precision within an assay): CV% is less than 8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV% is less than 10%
Three samples of known concentration were tested in twenty assays to assess.

Molecular Weight

84,863 Da

3D Structure

ModBase 3D Structure for P59667

Detection Wavelength

450 nm

Sample Volume

50-100ul

Protein Biological Process 1

Transcription/Transcription regulation

Protein Biological Process 3

Transcription

ISO Certification

Manufactured in an ISO 9001:2015 Certified Laboratory.

Other Notes

Small volumes of NR3C1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for NR3C1 purchase

MBS700327 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the nuclear receptor subfamily 3, group C, member 1 (glucocorticoid receptor) (NR3C1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing NR3C1. The ELISA analytical biochemical technique of the MBS700327 kit is based on NR3C1 antibody-NR3C1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect NR3C1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, NR3C1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for NR3C1. It may not necessarily be applicable to this product.

NCBI GI #

126723281

NCBI GeneID

100008890

NCBI Accession #

NP_001075616.1

UniProt Primary Accession #

P59667

UniProt Related Accession #

P59667

GenBank Nucleotide #

NM_001082147.1

NCBI Official Full Name

glucocorticoid receptor

NCBI Official Symbol

NR3C1

UniProt Protein Name

Glucocorticoid receptor

UniProt Synonym Protein Names

Nuclear receptor subfamily 3 group C member 1

Protein Family

Glucocorticoid receptor

UniProt Gene Name

NR3C1

UniProt Synonym Gene Names

GRL; GR

UniProt Entry Name

GCR_RABIT

NCBI Protein Information

glucocorticoid receptor; GR; glucocorticoid receptor alpha; nuclear receptor subfamily 3 group C member 1

UniProt Comments for NR3C1

Function: Receptor for glucocorticoids (GC). Has a dual mode of action: as a transcription factor that binds to glucocorticoid response elements (GRE), both for nuclear and mitochondrial DNA, and as a modulator of other transcription factors. Affects inflammatory responses, cellular proliferation and differentiation in target tissues. Could act as a coactivator for STAT5-dependent transcription upon growth hormone (GH) stimulation and could reveal an essential role of hepatic GR in the control of body growth. Involved in chromatin remodeling. May play a negative role in adipogenesis through the regulation of lipolytic and antilipogenic genes expression

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with NR3C1 elisa kit

Reference Product	PubMed Publications
JUN elisa kit	>21 publications with NR3C1 and JUN
FOS elisa kit	>3 publications with NR3C1 and FOS
STAT3 elisa kit	>2 publications with NR3C1 and STAT3

Pathways associated with NR3C1 elisa kit

Products by Pathway	Pathway Diagram
Adipogenesis Pathway antibodies	Adipogenesis Pathway Diagram
Androgen Receptor Signaling Pathway antibodies	Androgen Receptor Signaling Pathway Diagram
BMAL1:CLOCK/NPAS2 Activates Circadian Expression Pathway antibodies	BMAL1:CLOCK/NPAS2 Activates Circadian Expression Pathway Diagram
Circadian Clock Pathway antibodies	Circadian Clock Pathway Diagram
FOXA2 And FOXA3 Transcription Factor Networks Pathway antibodies	FOXA2 And FOXA3 Transcription Factor Networks Pathway Diagram
Gene Expression Pathway antibodies	Gene Expression Pathway Diagram
Generic Transcription Pathway antibodies	Generic Transcription Pathway Diagram
Glucocorticoid Receptor Regulatory Network Pathway antibodies	Glucocorticoid Receptor Regulatory Network Pathway Diagram
IL-2 Signaling Pathway antibodies	IL-2 Signaling Pathway Diagram
Neuroactive Ligand-receptor Interaction Pathway antibodies	Neuroactive Ligand-receptor Interaction Pathway Diagram

Diseases associated with NR3C1 elisa kit

Disease Name	Pubmed Publications
Inflammation Antibodies	>21 publications with NR3C1 and Inflammation
Nervous System Diseases Antibodies	>19 publications with NR3C1 and Nervous System Diseases
Depressive Disorder Antibodies	>17 publications with NR3C1 and Depressive Disorder
Hypertension Antibodies	>16 publications with NR3C1 and Hypertension
Cardiovascular Diseases Antibodies	>14 publications with NR3C1 and Cardiovascular Diseases
Bipolar Disorder Antibodies	>12 publications with NR3C1 and Bipolar Disorder
Lung Diseases, Obstructive Antibodies	>11 publications with NR3C1 and Lung Diseases, Obstructive
Necrosis Antibodies	>10 publications with NR3C1 and Necrosis
Brain Diseases Antibodies	>10 publications with NR3C1 and Brain Diseases
Muscular Diseases Antibodies	>8 publications with NR3C1 and Muscular Diseases

Organs/Tissues associated with NR3C1 elisa kit

Organ/Tissue Name	Pubmed Publications
Blood Antibodies	>40 publications with NR3C1 and Blood
Brain Antibodies	>30 publications with NR3C1 and Brain
Liver Antibodies	>12 publications with NR3C1 and Liver
Skin Antibodies	>7 publications with NR3C1 and Skin
Heart Antibodies	>6 publications with NR3C1 and Heart
Intestine Antibodies	>5 publications with NR3C1 and Intestine
Placenta Antibodies	>5 publications with NR3C1 and Placenta
Vascular Antibodies	>4 publications with NR3C1 and Vascular
Muscle Antibodies	>4 publications with NR3C1 and Muscle
Pituitary Gland Antibodies	>4 publications with NR3C1 and Pituitary Gland