Heat Shock Protein 90kDa Alpha (Cytosolic), Class A Member 1 (HSP-90) ELISA Kit for Rat

Name: Rat HSP-90 ELISA Kit; MyBioSource
Brand: MyBioSource
SKU: MBS700359
Price: 295 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS700359

Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for Hsp90aa1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any Hsp90aa1 present is bound by the… More details

$295 / 24-Strip-Wells (LIMIT 1) +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Request Current Manual

Product Overview

Online Datasheet in PDF

Product Name

heat shock protein 90kDa alpha (cytosolic) , class A member 1 (HSP-90), ELISA Kit

Full Product Name

Rat Heat shock protein HSP 90-alpha, Hsp90aa1/Hsp86/Hspca ELISA Kit

Product Gene Name

HSP-90 elisa kit

Product Synonym Names

Rat Heat shock protein HSP 90-alpha (Hsp90aa1/Hsp86/Hspca) ELISA kit; FLJ31884; HSP86; HSP89A; HSP90A; HSP90N; HSPC1; HSPCA; HSPCAL1; HSPCAL4; HSPN; Hsp89; Hsp90; LAP2; heat shock 90kD protein 1; alpha; heat shock 90kD protein 1; alpha-like 4; Heat shock protein HSP 90-alpha; heat shock protein 90kDa alpha (cytosolic); class A member 1

Species Reactivity

Rat

Assay Type

Sandwich

Samples

Serum, plasma, tissue homogenates

Detection Range

0.312 ng/ml-20 ng/ml.

Sensitivity

The minimum detectable dose of rat Hsp90aa1 is typically less than 0.078 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.

Specificity

This assay has high sensitivity and excellent specificity for detection of rat Hsp90aa1. No significant cross-reactivity or interference between rat Hsp90aa1 and analogues was observed.

Preparation and Storage

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Inter-assay Precision

Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

Intra-assay Precision

Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.

Molecular Weight

79,733 Da

3D Structure

ModBase 3D Structure for P30946

ISO Certification

Manufactured in an ISO 9001:2015 Certified Laboratory.

Other Notes

Small volumes of HSP-90 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for HSP-90 purchase

MBS700359 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the heat shock protein 90kDa alpha (cytosolic), class A member 1 (HSP-90) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing HSP-90. The ELISA analytical biochemical technique of the MBS700359 kit is based on HSP-90 antibody-HSP-90 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect HSP-90 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, HSP-90. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for HSP-90. It may not necessarily be applicable to this product.

NCBI GI #

338817950

NCBI Accession #

P30946.2

UniProt Primary Accession #

P30946

NCBI Official Full Name

Heat shock protein HSP 90-alpha

UniProt Protein Name

Heat shock protein HSP 90-alpha

UniProt Gene Name

HSP90AA1

UniProt Synonym Gene Names

HSPCA

UniProt Entry Name

HS90A_RABIT

UniProt Comments for HSP-90

Function: Molecular chaperone that promotes the maturation, structural maintenance and proper regulation of specific target proteins involved for instance in cell cycle control and signal transduction. Undergoes a functional cycle that is linked to its ATPase activity. This cycle probably induces conformational changes in the client proteins, thereby causing their activation. Interacts dynamically with various co-chaperones that modulate its substrate recognition, ATPase cycle and chaperone function Binds bacterial lipopolysaccharide (LPS) et mediates LPS-induced inflammatory response, including TNF secretion UniRule annotationHAMAP-Rule:MF_00505

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with HSP-90 elisa kit

Reference Product	PubMed Publications
HSPA8 elisa kit	>6 publications with HSP-90 and HSPA8
STIP1 elisa kit	>2 publications with HSP-90 and STIP1
HSPA4 elisa kit	>1 publications with HSP-90 and HSPA4

Pathways associated with HSP-90 elisa kit

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Nucleotide-binding Domain, Leucine Rich Repeat Containing Receptor (NLR) Signaling Pathways antibodies	Nucleotide-binding Domain, Leucine Rich Repeat Containing Receptor (NLR) Signaling Pathways Diagram

Diseases associated with HSP-90 elisa kit

Disease Name	Pubmed Publications
Neoplasms Antibodies	>15 publications with HSP-90 and Neoplasms
Carcinoma Antibodies	>7 publications with HSP-90 and Carcinoma
Gonadal Disorders Antibodies	>4 publications with HSP-90 and Gonadal Disorders
Immune System Diseases Antibodies	>4 publications with HSP-90 and Immune System Diseases
Inflammation Antibodies	>3 publications with HSP-90 and Inflammation
Disease Models, Animal Antibodies	>3 publications with HSP-90 and Disease Models, Animal
Liver Neoplasms Antibodies	>3 publications with HSP-90 and Liver Neoplasms
Ovarian Neoplasms Antibodies	>3 publications with HSP-90 and Ovarian Neoplasms
Adenocarcinoma Antibodies	>3 publications with HSP-90 and Adenocarcinoma
Liver Diseases Antibodies	>3 publications with HSP-90 and Liver Diseases

Organs/Tissues associated with HSP-90 elisa kit

Organ/Tissue Name	Pubmed Publications
Blood Antibodies	>5 publications with HSP-90 and Blood
Brain Antibodies	>5 publications with HSP-90 and Brain
Intestine Antibodies	>4 publications with HSP-90 and Intestine
Ovary Antibodies	>4 publications with HSP-90 and Ovary
Kidney Antibodies	>3 publications with HSP-90 and Kidney
Liver Antibodies	>3 publications with HSP-90 and Liver
Embryonic Tissue Antibodies	>3 publications with HSP-90 and Embryonic Tissue
Bone Antibodies	>3 publications with HSP-90 and Bone
Bone Marrow Antibodies	>3 publications with HSP-90 and Bone Marrow
Testis Antibodies	>3 publications with HSP-90 and Testis