Transient Receptor Potential Cation Channel, Subfamily V, Member 4 (TRPV4) ELISA Kit for Human

Name: Human TRPV4 ELISA Kit; MyBioSource
Brand: MyBioSource
SKU: MBS700415
Price: 295 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS700415

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for TRPV2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any TRPV2 present is bound by the… More details

$295 / 24-Strip-Wells (LIMIT 1) +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

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Product Overview

Online Datasheet in PDF

Product Name

transient receptor potential cation channel, subfamily V, member 4 (TRPV4), ELISA Kit

Full Product Name

Human transient receptor potential Cation channel subfamily V member 4, TrpV4 ELISA Kit

Product Gene Name

TRPV4 elisa kit

Product Synonym Names

Human transient receptor potential cation channel subfamily V member 4 (TrpV4) ELISA Kit; OTRPC4; TRP12; VR-OAC; VRL-2; VRL2; VROAC; OSM9-like transient receptor potential channel 4; osmosensitive transient receptor potential channel 4; transient receptor potential channel 12; vanilloid rec; transient receptor potential cation channel; subfamily V; member 4

Species Reactivity

Human

Assay Type

Quantitative Sandwich

Samples

Serum, Plasma, Tissue Homogenates, Cell Lysates

Detection Range

25 pg/ml-1600 pg/ml

Sensitivity

6.25 pg/ml.

Specificity

This assay has high sensitivity and excellent specificity for detection of human TRPV2. No significant cross-reactivity or interference between human TRPV2 and analogues was observed.

Preparation and Storage

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Inter-assay Precision

Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

Intra-assay Precision

Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess

Molecular Weight

98,281 Da

OMIM

613508

3D Structure

ModBase 3D Structure for Q9HBA0

ISO Certification

Manufactured in an ISO 9001:2015 Certified Laboratory.

Other Notes

Small volumes of TRPV4 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for TRPV4 purchase

MBS700415 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the transient receptor potential cation channel, subfamily V, member 4 (TRPV4) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing TRPV4. The ELISA analytical biochemical technique of the MBS700415 kit is based on TRPV4 antibody-TRPV4 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect TRPV4 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, TRPV4. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for TRPV4. It may not necessarily be applicable to this product.

NCBI GI #

294459965

NCBI GeneID

59341

NCBI Accession #

NP_001170899.1

UniProt Primary Accession #

Q9HBA0

UniProt Secondary Accession #

Q17R79; Q2Y122; Q2Y123; Q2Y124; Q86YZ6; Q8NDY7; Q8NG64; Q96Q92; Q96RS7; Q9HBC0; B7ZKQ6

UniProt Related Accession #

Q9HBA0

GenBank Nucleotide #

NM_001177428.1

NCBI Official Full Name

transient receptor potential cation channel subfamily V member 4 isoform e

NCBI Official Synonym Full Names

transient receptor potential cation channel, subfamily V, member 4

NCBI Official Symbol

TRPV4

NCBI Official Synonym Symbols

SMAL; VRL2; CMT2C; SPSMA; TRP12; VROAC; HMSN2C; OTRPC4; SSQTL1

UniProt Protein Name

Transient receptor potential cation channel subfamily V member 4

UniProt Synonym Protein Names

Osm-9-like TRP channel 4; OTRPC4; Transient receptor potential protein 12; TRP12; Vanilloid receptor-like channel 2; Vanilloid receptor-like protein 2; VRL-2; Vanilloid receptor-related osmotically-activated channel; VR-OAC

Protein Family

Transient receptor potential cation channel subfamily

UniProt Gene Name

TRPV4

UniProt Synonym Gene Names

VRL2; VROAC; TrpV4; OTRPC4; TRP12; VRL-2; VR-OAC

UniProt Entry Name

TRPV4_HUMAN

NCBI Protein Information

transient receptor potential cation channel subfamily V member 4; osm-9-like TRP channel 4; vanilloid receptor-like channel 2; transient receptor potential protein 12; OSM9-like transient receptor potential channel 4; osmosensitive transient receptor potential channel 4; vanilloid receptor-related osmotically activated channel

NCBI Summary for TRPV4

This gene encodes a member of the OSM9-like transient receptor potential channel (OTRPC) subfamily in the transient receptor potential (TRP) superfamily of ion channels. The encoded protein is a Ca2+-permeable, nonselective cation channel that is thought to be involved in the regulation of systemic osmotic pressure. Mutations in this gene are the cause of spondylometaphyseal and metatropic dysplasia and hereditary motor and sensory neuropathy type IIC. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Apr 2010]

UniProt Comments for TRPV4

Function: Non-selective calcium permeant cation channel probably involved in osmotic sensitivity and mechanosensitivity. Activation by exposure to hypotonicity within the physiological range exhibits an outward rectification. Also activated by low pH, citrate and phorbol esters. Increase of intracellular Ca2+ potentiates currents. Channel activity seems to be regulated by a calmodulin-dependent mechanism with a negative feedback mechanism. Promotes cell-cell junction formation in skin keratinocytes and plays an important role in the formation and/or maintenance of functional intercellular barriers. Acts as a regulator of intracellular Ca2+ in synoviocytes. Plays an obligatory role as a molecular component in the nonselective cation channel activation induced by 4-alpha-phorbol 12,13-didecanoate and hypotonic stimulation in synoviocytes and also regulates production of IL-8.3 PublicationsManual assertion based on experiment in:Ref.2

Research Articles on TRPV4

1. Results show that TRPV4 activation in the intestinal epithelium caused epithelial hyperpermeability in response to NSAID-induced arachidonic acid metabolites and contributed to NSAID-induced intestinal damage.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with TRPV4 elisa kit

Reference Product	PubMed Publications
SRC elisa kit	>8 publications with TRPV4 and SRC
ITCH elisa kit	>4 publications with TRPV4 and ITCH
PACSIN3 elisa kit	>2 publications with TRPV4 and PACSIN3
LYN elisa kit	>2 publications with TRPV4 and LYN
PACSIN2 elisa kit	>1 publications with TRPV4 and PACSIN2
PACSIN1 elisa kit	>1 publications with TRPV4 and PACSIN1
MAP7 elisa kit	>1 publications with TRPV4 and MAP7

Pathways associated with TRPV4 elisa kit

Products by Pathway	Pathway Diagram
Inflammatory Mediator Regulation Of TRP Channels Pathway antibodies	Inflammatory Mediator Regulation Of TRP Channels Pathway Diagram
Inflammatory Mediator Regulation Of TRP Channels Pathway antibodies	Inflammatory Mediator Regulation Of TRP Channels Pathway Diagram
Ion Channel Transport Pathway antibodies	Ion Channel Transport Pathway Diagram
Stimuli-sensing Channels Pathway antibodies	Stimuli-sensing Channels Pathway Diagram
TRP Channels Pathway antibodies	TRP Channels Pathway Diagram
Transmembrane Transport Of Small Molecules Pathway antibodies	Transmembrane Transport Of Small Molecules Pathway Diagram

Diseases associated with TRPV4 elisa kit

Disease Name	Pubmed Publications
Nervous System Diseases Antibodies	>120 publications with TRPV4 and Nervous System Diseases
Pain Antibodies	>117 publications with TRPV4 and Pain
Inflammation Antibodies	>65 publications with TRPV4 and Inflammation
Hypertension Antibodies	>47 publications with TRPV4 and Hypertension
Hyperalgesia Antibodies	>45 publications with TRPV4 and Hyperalgesia
Disease Models, Animal Antibodies	>38 publications with TRPV4 and Disease Models, Animal
Bone Diseases Antibodies	>29 publications with TRPV4 and Bone Diseases
Muscular Atrophy, Spinal Antibodies	>21 publications with TRPV4 and Muscular Atrophy, Spinal
SCAPULOPERONEAL SPINAL MUSCULAR ATROPHY Antibodies	>21 publications with TRPV4 and SCAPULOPERONEAL SPINAL MUSCULAR ATROPHY
Charcot-Marie-Tooth Disease Antibodies	>18 publications with TRPV4 and Charcot-Marie-Tooth Disease

Organs/Tissues associated with TRPV4 elisa kit

Organ/Tissue Name	Pubmed Publications
Kidney Antibodies	>56 publications with TRPV4 and Kidney
Brain Antibodies	>47 publications with TRPV4 and Brain
Skin Antibodies	>38 publications with TRPV4 and Skin
Lung Antibodies	>37 publications with TRPV4 and Lung
Bone Antibodies	>27 publications with TRPV4 and Bone
Heart Antibodies	>19 publications with TRPV4 and Heart
Intestine Antibodies	>13 publications with TRPV4 and Intestine
Liver Antibodies	>9 publications with TRPV4 and Liver
Mouth Antibodies	>7 publications with TRPV4 and Mouth
Embryonic Tissue Antibodies	>5 publications with TRPV4 and Embryonic Tissue