Mouse CYP19A1 ELISA Kit – Cat# MBS700419

Cytochrome P450, Family 19, Subfamily A, Polypeptide 1 (CYP19A1) ELISA Kit for Mouse

Name: Mouse CYP19A1 ELISA Kit; MyBioSource
Brand: MyBioSource
SKU: MBS700419
Price: 295 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS700419

Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for ARO has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any ARO present is bound by the… More details

$295 / 24-Strip-Wells (LIMIT 1) +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

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Product Overview

Online Datasheet in PDF

Product Name

cytochrome P450, family 19, subfamily A, polypeptide 1 (CYP19A1), ELISA Kit

Full Product Name

Mouse Aromatase, ARO ELISA Kit

Product Gene Name

CYP19A1 elisa kit

Product Synonym Names

Mouse Aromatase (ARO) ELISA Kit; ARO; ARO1; CPV1; CYAR; CYP19; MGC104309; P-450AROM; OTTHUMP00000162543; aromatase; cytochrome P450; subfamily XIX (aromatization of androgens) ; estrogen synthetase; flavoprotein-linked monooxygenase; mic; cytochrome P450; family 19; subfamily A; polypeptide 1

Species Reactivity

Mouse

Assay Type

Sandwich

Samples

Serum, plasma, tissue homogenates

Detection Range

6.25 pg/ml-400 pg/ml.

Sensitivity

The minimum detectable dose of mouse ARO is typically less than 1.56 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.

Specificity

This assay has high sensitivity and excellent specificity for detection of mouse ARO. No significant cross-reactivity or interference between mouse ARO and analogues was observed.

Preparation and Storage

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Inter-assay Precision

Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

Intra-assay Precision

Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.

Molecular Weight

58,015 Da

3D Structure

ModBase 3D Structure for P28649

ISO Certification

Manufactured in an ISO 9001:2015 Certified Laboratory.

Other Notes

Small volumes of CYP19A1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for CYP19A1 purchase

MBS700419 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the cytochrome P450, family 19, subfamily A, polypeptide 1 (CYP19A1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing CYP19A1. The ELISA analytical biochemical technique of the MBS700419 kit is based on CYP19A1 antibody-CYP19A1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect CYP19A1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, CYP19A1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for CYP19A1. It may not necessarily be applicable to this product.

NCBI GI #

6681099

NCBI GeneID

13075

NCBI Accession #

NP_031836.1

UniProt Primary Accession #

P28649

UniProt Related Accession #

P28649

GenBank Nucleotide #

NM_007810.3

NCBI Official Full Name

aromatase

NCBI Official Synonym Full Names

cytochrome P450, family 19, subfamily a, polypeptide 1

NCBI Official Symbol

Cyp19a1

NCBI Official Synonym Symbols

Ar; ArKO; Int5; Cyp19; Int-5; p450arom

UniProt Protein Name

Aromatase

UniProt Synonym Protein Names

CYPXIX; Cytochrome P-450AROM; Cytochrome P450 19A1; Estrogen synthase

Protein Family

Aromatase

UniProt Gene Name

Cyp19a1

UniProt Synonym Gene Names

Arom; Cyp19

UniProt Entry Name

CP19A_MOUSE

NCBI Protein Information

aromatase; CYPXIX; estrogen synthase; cytochrome P-450AROM; cytochrome P450 19A1; cytochrome P450, 19, aromatase

UniProt Comments for CYP19A1

Function: Catalyzes the formation of aromatic C18 estrogens from C19 androgens.

Catalytic activity: Testosterone + 3 O2 + 3 reduced flavoproteins = 17-beta-estradiol + formate + 4 H2O + 3 oxidized flavoproteins.

Cofactor: Heme group

Research Articles on CYP19A1

1. Cerebrovascular endothelial aromatase plays an important and sexually dimorphic role in cerebrovascular function and that aromatase inhibitors in clinical use may have cardiovascular consequences in both males and females.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with CYP19A1 elisa kit

Reference Product	PubMed Publications
Srd5a2 elisa kit	>153 publications with CYP19A1 and Srd5a2
Hsd17b1 elisa kit	>49 publications with CYP19A1 and Hsd17b1
Cyp11b2 elisa kit	>42 publications with CYP19A1 and Cyp11b2
Hsd3b1 elisa kit	>42 publications with CYP19A1 and Hsd3b1
Hsd3b2 elisa kit	>15 publications with CYP19A1 and Hsd3b2
Srd5a1 elisa kit	>13 publications with CYP19A1 and Srd5a1
Hsd17b3 elisa kit	>13 publications with CYP19A1 and Hsd17b3
Cyp2b10 elisa kit	>2 publications with CYP19A1 and Cyp2b10
Hsd17b6 elisa kit	>2 publications with CYP19A1 and Hsd17b6

Pathways associated with CYP19A1 elisa kit

Products by Pathway	Pathway Diagram
Biological Oxidations Pathway antibodies	Biological Oxidations Pathway Diagram
C19/C18-Steroid Hormone Biosynthesis, Pregnenolone => Androstenedione => Estrone Pathway antibodies	C19/C18-Steroid Hormone Biosynthesis, Pregnenolone => Androstenedione => Estrone Pathway Diagram
C19/C18-Steroid Hormone Biosynthesis, Pregnenolone => Androstenedione => Estrone Pathway antibodies	C19/C18-Steroid Hormone Biosynthesis, Pregnenolone => Androstenedione => Estrone Pathway Diagram
Cytochrome P450 Pathway antibodies	Cytochrome P450 Pathway Diagram
Cytochrome P450 - Arranged By Substrate Type Pathway antibodies	Cytochrome P450 - Arranged By Substrate Type Pathway Diagram
Endogenous Sterols Pathway antibodies	Endogenous Sterols Pathway Diagram
Estrogen Biosynthesis Pathway antibodies	Estrogen Biosynthesis Pathway Diagram
Metabolic Pathways antibodies	Metabolic Pathways Diagram
Metabolism Pathway antibodies	Metabolism Pathway Diagram
Metabolism Of Lipids And Lipoproteins Pathway antibodies	Metabolism Of Lipids And Lipoproteins Pathway Diagram

Diseases associated with CYP19A1 elisa kit

Disease Name	Pubmed Publications
Breast Neoplasms Antibodies	>4083 publications with CYP19A1 and Breast Neoplasms
Ovarian Diseases Antibodies	>454 publications with CYP19A1 and Ovarian Diseases
Osteoporosis Antibodies	>424 publications with CYP19A1 and Osteoporosis
Adenocarcinoma Antibodies	>390 publications with CYP19A1 and Adenocarcinoma
Neoplasms, Experimental Antibodies	>373 publications with CYP19A1 and Neoplasms, Experimental
Infertility, Female Antibodies	>352 publications with CYP19A1 and Infertility, Female
Pain Antibodies	>333 publications with CYP19A1 and Pain
Endometriosis Antibodies	>316 publications with CYP19A1 and Endometriosis
Hyperplasia Antibodies	>277 publications with CYP19A1 and Hyperplasia
Nervous System Diseases Antibodies	>274 publications with CYP19A1 and Nervous System Diseases

Organs/Tissues associated with CYP19A1 elisa kit

Organ/Tissue Name	Pubmed Publications
Ovary Antibodies	>2079 publications with CYP19A1 and Ovary
Blood Antibodies	>1807 publications with CYP19A1 and Blood
Brain Antibodies	>1459 publications with CYP19A1 and Brain
Testis Antibodies	>868 publications with CYP19A1 and Testis
Placenta Antibodies	>782 publications with CYP19A1 and Placenta
Liver Antibodies	>422 publications with CYP19A1 and Liver
Embryonic Tissue Antibodies	>293 publications with CYP19A1 and Embryonic Tissue
Vascular Antibodies	>195 publications with CYP19A1 and Vascular
Adrenal Gland Antibodies	>179 publications with CYP19A1 and Adrenal Gland
Lung Antibodies	>117 publications with CYP19A1 and Lung