Keratin, Type 1 Cytoskeletal 18 Fragment (K18-ASP396) ELISA Kit for Rat

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for K18-Asp396 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any K18-Asp396 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for K18-Asp396 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of K18-Asp396 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Small volumes of K18-Asp396 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS700460 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Keratin, type I cytoskeletal 18 fragment (K18-Asp396) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing K18-Asp396. The ELISA analytical biochemical technique of the MBS700460 kit is based on K18-Asp396 antibody-K18-Asp396 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect K18-Asp396 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, K18-Asp396. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).