G Protein-Coupled Bile Acid Receptor 1 (GPBAR1) ELISA Kit for Human

Name: Human GPBAR1 ELISA Kit; G Protein-Coupled Bile Acid Receptor 1; MyBioSource
Brand: MyBioSource
SKU: MBS700478
Price: 295 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS700478

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to GPBAR1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation… More details

$295 / 24-Strip-Wells (LIMIT 1) +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

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Product Overview

Online Datasheet in PDF

Product Name

G protein-coupled bile acid receptor 1 (GPBAR1), ELISA Kit

Full Product Name

Human G protein-coupled bile acid receptor 1, GPBAR1 ELISA Kit

Product Gene Name

GPBAR1 elisa kit

Product Synonym Names

Human G protein-coupled bile acid receptor 1 (GPBAR1) ELISA Kit; BG37; GPCR; GPCR19; GPR131; M-BAR; MGC40597; TGR5; G-protein coupled bile acid receptor BG37; membrane bile acid receptor; membrane-type receptor for bile acids; G protein-coupled bile acid receptor 1

Species Reactivity

Human

Detection Range

0.156 ng/ml-10 ng/ml

Sensitivity

0.039 ng/ml

Specificity

This assay has high sensitivity and excellent specificity for detection of Human GPBAR1. No significant cross-reactivity or interference between Human GPBAR1 and analogues was observed.

Preparation and Storage

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Intra-assay Precision

Intra-assay Precision (Precision within an assay): CV% is less than 8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV% is less than 10%
Three samples of known concentration were tested in twenty assays to assess.

Molecular Weight

35,248 Da

OMIM

610147

3D Structure

ModBase 3D Structure for Q8TDU6

Detection Wavelength

450 nm

Sample Volume

50-100ul

ISO Certification

Manufactured in an ISO 9001:2015 Certified Laboratory.

Other Notes

Small volumes of GPBAR1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for GPBAR1 purchase

MBS700478 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the G protein-coupled bile acid receptor 1 (GPBAR1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing GPBAR1. The ELISA analytical biochemical technique of the MBS700478 kit is based on GPBAR1 antibody-GPBAR1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect GPBAR1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, GPBAR1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for GPBAR1. It may not necessarily be applicable to this product.

NCBI GI #

116284384

NCBI GeneID

151306

NCBI Accession #

NP_001070659.1

UniProt Primary Accession #

Q8TDU6

UniProt Secondary Accession #

B3KV35

UniProt Related Accession #

Q8TDU6

GenBank Nucleotide #

NM_001077191.1

NCBI Official Full Name

G-protein coupled bile acid receptor 1

NCBI Official Synonym Full Names

G protein-coupled bile acid receptor 1

NCBI Official Symbol

GPBAR1

NCBI Official Synonym Symbols

BG37; TGR5; M-BAR; GPCR19; GPR131

UniProt Protein Name

G-protein coupled bile acid receptor 1

UniProt Synonym Protein Names

G-protein coupled receptor GPCR19; hGPCR19; Membrane-type receptor for bile acids; M-BAR; hBG37; BG37

Protein Family

G-protein coupled bile acid receptor

UniProt Gene Name

GPBAR1

UniProt Synonym Gene Names

TGR5; hGPCR19; M-BAR; BG37

UniProt Entry Name

GPBAR_HUMAN

NCBI Protein Information

G-protein coupled bile acid receptor 1; hBG37; hGPCR19; membrane bile acid receptor; G-protein coupled receptor GPCR19; membrane-type receptor for bile acids; G-protein coupled bile acid receptor BG37

NCBI Summary for GPBAR1

This gene encodes a member of the G protein-coupled receptor (GPCR) superfamily. This enzyme functions as a cell surface receptor for bile acids. Treatment of cells expressing this GPCR with bile acids induces the production of intracellular cAMP, activation of a MAP kinase signaling pathway, and internalization of the receptor. The receptor is implicated in the suppression of macrophage functions and regulation of energy homeostasis by bile acids. Alternative splicing results in multiple transcript variants encoding the same protein. [provided by RefSeq, Jul 2008]

UniProt Comments for GPBAR1

Function: Receptor for bile acid. Bile acid-binding induces its internalization, activation of extracellular signal-regulated kinase and intracellular cAMP production. May be involved in the suppression of macrophage functions by bile acids.2 PublicationsManual assertion based on experiment in:Ref.1

Research Articles on GPBAR1

1. the secondary structure of the TGR5 membrane-proximal C terminus is the determining factor for plasma membrane localization and responsiveness towards extracellular ligands.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with GPBAR1 elisa kit

Reference Product	PubMed Publications
GCG elisa kit	>1 publications with GPBAR1 and GCG

Pathways associated with GPBAR1 elisa kit

Products by Pathway	Pathway Diagram
Class A/1 (Rhodopsin-like Receptors) Pathway antibodies	Class A/1 (Rhodopsin-like Receptors) Pathway Diagram
G Alpha (s) Signalling Events Pathway antibodies	G Alpha (s) Signalling Events Pathway Diagram
GPCR Downstream Signaling Pathway antibodies	GPCR Downstream Signaling Pathway Diagram
GPCR Ligand Binding Pathway antibodies	GPCR Ligand Binding Pathway Diagram
Signal Transduction Pathway antibodies	Signal Transduction Pathway Diagram
Signaling By GPCR Pathway antibodies	Signaling By GPCR Pathway Diagram

Diseases associated with GPBAR1 elisa kit

Disease Name	Pubmed Publications
Liver Diseases Antibodies	>28 publications with GPBAR1 and Liver Diseases
Inflammation Antibodies	>19 publications with GPBAR1 and Inflammation
Fatty Liver Antibodies	>10 publications with GPBAR1 and Fatty Liver
Cholestasis Antibodies	>9 publications with GPBAR1 and Cholestasis
Necrosis Antibodies	>7 publications with GPBAR1 and Necrosis
Carcinoma Antibodies	>7 publications with GPBAR1 and Carcinoma
Liver Neoplasms Antibodies	>5 publications with GPBAR1 and Liver Neoplasms
Gallstones Antibodies	>5 publications with GPBAR1 and Gallstones
Fibrosis Antibodies	>4 publications with GPBAR1 and Fibrosis
Cholestasis, Intrahepatic Antibodies	>3 publications with GPBAR1 and Cholestasis, Intrahepatic

Organs/Tissues associated with GPBAR1 elisa kit

Organ/Tissue Name	Pubmed Publications
Blood Antibodies	>14 publications with GPBAR1 and Blood
Connective Tissue Antibodies	>4 publications with GPBAR1 and Connective Tissue
Brain Antibodies	>3 publications with GPBAR1 and Brain
Nerve Antibodies	>1 publications with GPBAR1 and Nerve
Lung Antibodies	>1 publications with GPBAR1 and Lung