Malate Dehydrogenase 1, NAD (Soluble) (MDH1) ELISA Kit for Human

Name: Human MDH1 ELISA Kit; Malate Dehydrogenase 1, NAD (Soluble); MyBioSource
Brand: MyBioSource
SKU: MBS700499
Price: 295 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS700499

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for MDH1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any MDH1 present is bound by the… More details

$295 / 24-Strip-Wells (LIMIT 1) +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Request Current Manual

Product Overview

Online Datasheet in PDF

Product Name

malate dehydrogenase 1, NAD (soluble) (MDH1), ELISA Kit

Full Product Name

Human Malate Dehydrogenase 1, MDH1 ELISA Kit

Product Gene Name

MDH1 elisa kit

Product Synonym Names

Human Malate Dehydrogenase 1 (MDH1) ELISA Kit; MDH-s; MDHA; MGC:1375; MOR2; cytosolic malate dehydrogenase; soluble malate dehydrogenase; malate dehydrogenase 1; NAD (soluble)

Species Reactivity

Human

Assay Type

Sandwich

Samples

Serum, plasma, tissue homogenates and Cell lysates

Detection Range

9.38 mU/ml-600 mU/ml

Sensitivity

2.35 mU/ml

Specificity

This assay has high sensitivity and excellent specificity for detection of Human MDH1. No significant cross-reactivity or interference between Human MDH1 and analogues was observed.

Preparation and Storage

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Intra-assay Precision

Intra-assay Precision (Precision within an assay): CV% is less than 8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV% is less than 10%
Three samples of known concentration were tested in twenty assays to assess.

Molecular Weight

36,426 Da

OMIM

154200

3D Structure

ModBase 3D Structure for P40925

Detection Wavelength

450 nm

Sample Volume

50-100ul

Protein Biological Process 1

Biosynthesis/Metabolism

Protein Biological Process 3

Tricarboxylic acid cycle

ISO Certification

Manufactured in an ISO 9001:2015 Certified Laboratory.

Other Notes

Small volumes of MDH1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for MDH1 purchase

MBS700499 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the malate dehydrogenase 1, NAD (soluble) (MDH1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing MDH1. The ELISA analytical biochemical technique of the MBS700499 kit is based on MDH1 antibody-MDH1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect MDH1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, MDH1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for MDH1. It may not necessarily be applicable to this product.

NCBI GI #

312283701

NCBI GeneID

4190

NCBI Accession #

NP_001186040.1

UniProt Primary Accession #

P40925

UniProt Secondary Accession #

Q6I9V0; B2R5V5; B4DUN2; B7Z3I7; F5H098

UniProt Related Accession #

P40925

GenBank Nucleotide #

NM_001199111.1

NCBI Official Full Name

malate dehydrogenase, cytoplasmic isoform 1

NCBI Official Synonym Full Names

malate dehydrogenase 1, NAD (soluble)

NCBI Official Symbol

MDH1

NCBI Official Synonym Symbols

MDHA; MOR2; MDH-s; HEL-S-32; MGC:1375

UniProt Protein Name

Malate dehydrogenase, cytoplasmic

UniProt Synonym Protein Names

Cytosolic malate dehydrogenase; Diiodophenylpyruvate reductase (EC:1.1.1.96)

Protein Family

Malate dehydrogenase

UniProt Gene Name

MDH1

UniProt Synonym Gene Names

MDHA

UniProt Entry Name

MDHC_HUMAN

NCBI Protein Information

malate dehydrogenase, cytoplasmic; soluble malate dehydrogenase; cytosolic malate dehydrogenase; diiodophenylpyruvate reductase; epididymis secretory protein Li 32

NCBI Summary for MDH1

Malate dehydrogenase catalyzes the reversible oxidation of malate to oxaloacetate, utilizing the NAD/NADH cofactor system in the citric acid cycle. The protein encoded by this gene is localized to the cytoplasm and may play pivotal roles in the malate-aspartate shuttle that operates in the metabolic coordination between cytosol and mitochondria. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.[provided by RefSeq, Nov 2010]

UniProt Comments for MDH1

MDH1: Malate dehydrogenase catalyzes the reversible oxidation of malate to oxaloacetate, utilizing the NAD/NADH cofactor system in the citric acid cycle. The protein encoded by this gene is localized to the cytoplasm and may play pivotal roles in the malate-aspartate shuttle that operates in the metabolic coordination between cytosol and mitochondria. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.[provided by RefSeq, Nov 2010]

Protein type: Carbohydrate Metabolism - pyruvate; Oxidoreductase; EC 1.1.1.37; EC 1.1.1.96; Carbohydrate Metabolism - glyoxylate and dicarboxylate; Carbohydrate Metabolism - citrate (TCA) cycle

Chromosomal Location of Human Ortholog: 2p13.3

Cellular Component: extracellular space; centrosome; mitochondrion; cytoplasm; cytosol

Molecular Function: malic enzyme activity; L-malate dehydrogenase activity; diiodophenylpyruvate reductase activity; NAD binding

Biological Process: malate metabolic process; oxaloacetate metabolic process; NADH metabolic process; tricarboxylic acid cycle; carbohydrate metabolic process; glucose metabolic process; cellular carbohydrate metabolic process; pathogenesis; gluconeogenesis

Research Articles on MDH1

1. adipogenic differentiation may be regulated by the acetylation of MDH1

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with MDH1 elisa kit

Reference Product	PubMed Publications
ME1 elisa kit	>5 publications with MDH1 and ME1
GOT1 elisa kit	>5 publications with MDH1 and GOT1
GOT2 elisa kit	>3 publications with MDH1 and GOT2
PCK1 elisa kit	>1 publications with MDH1 and PCK1

Pathways associated with MDH1 elisa kit

Products by Pathway	Pathway Diagram
Carbon Metabolism Pathway antibodies	Carbon Metabolism Pathway Diagram
Carbon Metabolism Pathway antibodies	Carbon Metabolism Pathway Diagram
Citrate Cycle (TCA Cycle) Pathway antibodies	Citrate Cycle (TCA Cycle) Pathway Diagram
Citrate Cycle (TCA Cycle) Pathway antibodies	Citrate Cycle (TCA Cycle) Pathway Diagram
Citrate Cycle (TCA Cycle, Krebs Cycle) Pathway antibodies	Citrate Cycle (TCA Cycle, Krebs Cycle) Pathway Diagram
Citrate Cycle (TCA Cycle, Krebs Cycle) Pathway antibodies	Citrate Cycle (TCA Cycle, Krebs Cycle) Pathway Diagram
Citrate Cycle, Second Carbon Oxidation, 2-oxoglutarate => Oxaloacetate Pathway antibodies	Citrate Cycle, Second Carbon Oxidation, 2-oxoglutarate => Oxaloacetate Pathway Diagram
Citrate Cycle, Second Carbon Oxidation, 2-oxoglutarate => Oxaloacetate Pathway antibodies	Citrate Cycle, Second Carbon Oxidation, 2-oxoglutarate => Oxaloacetate Pathway Diagram
Cysteine And Methionine Metabolism Pathway antibodies	Cysteine And Methionine Metabolism Pathway Diagram
Cysteine And Methionine Metabolism Pathway antibodies	Cysteine And Methionine Metabolism Pathway Diagram

Diseases associated with MDH1 elisa kit

Disease Name	Pubmed Publications
Nervous System Diseases Antibodies	>5 publications with MDH1 and Nervous System Diseases
Brain Diseases Antibodies	>3 publications with MDH1 and Brain Diseases
Vascular Diseases Antibodies	>2 publications with MDH1 and Vascular Diseases
Heart Diseases Antibodies	>2 publications with MDH1 and Heart Diseases
Disease Models, Animal Antibodies	>2 publications with MDH1 and Disease Models, Animal
Dyslipidemias Antibodies	>1 publications with MDH1 and Dyslipidemias
Seizures Antibodies	>1 publications with MDH1 and Seizures
Kidney Diseases Antibodies	>1 publications with MDH1 and Kidney Diseases
Fibrosis Antibodies	>1 publications with MDH1 and Fibrosis
Inflammation Antibodies	>1 publications with MDH1 and Inflammation

Organs/Tissues associated with MDH1 elisa kit

Organ/Tissue Name	Pubmed Publications
Bone Antibodies	>13 publications with MDH1 and Bone
Connective Tissue Antibodies	>4 publications with MDH1 and Connective Tissue
Heart Antibodies	>3 publications with MDH1 and Heart
Muscle Antibodies	>3 publications with MDH1 and Muscle
Brain Antibodies	>3 publications with MDH1 and Brain
Vascular Antibodies	>2 publications with MDH1 and Vascular
Embryonic Tissue Antibodies	>2 publications with MDH1 and Embryonic Tissue
Ear Antibodies	>1 publications with MDH1 and Ear
Kidney Antibodies	>1 publications with MDH1 and Kidney
Umbilical Cord Antibodies	>1 publications with MDH1 and Umbilical Cord