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D-Amino-Acid Oxidase (DAO) ELISA Kit for Human

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS700670

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with anantibody specific to DAO. Standards or samples are then added to theappropriate microtiter plate wells with a biotin-conjugated polyclonalantibody preparation… More details

$295 / 24-Strip-Wells (LIMIT 1) +1 FREE 8GB USB

Product Documentation

PDF
Sample Manual Insert View PDF
PDF
Testing Data View PDF
PDF
Certificate of Analysis (COA) View PDF
PDF
QC Report View PDF
PDF
Material Safety Data Sheet View PDF
PDF
Technical Datasheet View PDF
PDF
Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
D-amino-acid oxidase (DAO), ELISA Kit
Full Product Name
Human diamine oxidase, DAO ELISA Kit
Product Gene Name
DAO elisa kit
Product Synonym Names
Human diamine oxidase; DAO ELISA Kit; DAAO; DAMOX; MGC35381; OXDA; ; D-amino-acid oxidase
Species Reactivity
Human
Samples
Serum, plasma, tissue homogenates
Detection Range
15.6 mIU/ml-1000 mIU/ml
Sensitivity
3.9 mIU/ml
Specificity
This assay has high sensitivity and excellent specificity for detection of Human DAO. No significant cross-reactivity or interference between Human DAO and analogues was observed.
Preparation and Storage
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
Intra-assay Precision
Intra-assay Precision (Precision within an assay): CV% is less than 8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV% is less than 10%
Three samples of known concentration were tested in twenty assays to assess.
Molecular Weight
39,474 Da
OMIM
124050
3D Structure
ModBase 3D Structure for P14920
Detection Wavelength
450 nm
Sample Volume
50-100ul
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.

Typical Testing Data/Standard Curve (for reference only) of DAO elisa kit

Human DAO ELISA Kit – Cat# MBS700670

Other Notes

Small volumes of DAO elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for DAO purchase

MBS700670 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the D-amino-acid oxidase (DAO) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing DAO. The ELISA analytical biochemical technique of the MBS700670 kit is based on DAO antibody-DAO antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect DAO antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, DAO. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for DAO. It may not necessarily be applicable to this product.

NCBI GI #
148539837
NCBI GeneID
1610
NCBI Accession #
NP_001908.3
UniProt Primary Accession #
P14920
UniProt Secondary Accession #
Q16758; Q8N6R2; B2R7I5
UniProt Related Accession #
P14920
GenBank Nucleotide #
NM_001917.4
NCBI Official Full Name
D-amino-acid oxidase
NCBI Official Synonym Full Names
D-amino-acid oxidase
NCBI Official Symbol
DAO
NCBI Official Synonym Symbols
DAAO; OXDA; DAMOX
UniProt Protein Name
D-amino-acid oxidase
Protein Family
2-oxoglutarate-dependent dioxygenase
UniProt Gene Name
DAO
UniProt Synonym Gene Names
DAMOX; DAAO; DAMOX; DAO
UniProt Entry Name
OXDA_HUMAN
NCBI Protein Information
D-amino-acid oxidase; D-amino acid oxidase

NCBI Summary for DAO

This gene encodes the peroxisomal enzyme D-amino acid oxidase. The enzyme is a flavoprotein which uses flavin adenine dinucleotide (FAD) as its prosthetic group. Its substrates include a wide variety of D-amino acids, but it is inactive on the naturally occurring L-amino acids. Its biological function is not known; it may act as a detoxifying agent which removes D-amino acids that accumulate during aging. In mice, it degrades D-serine, a co-agonist of the NMDA receptor. This gene may play a role in the pathophysiology of schizophrenia. [provided by RefSeq, Jul 2008]
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UniProt Comments for DAO

Function: Regulates the level of the neuromodulator D-serine in the brain. Has high activity towards D-DOPA and contributes to dopamine synthesis. Could act as a detoxifying agent which removes D-amino acids accumulated during aging. Acts on a variety of D-amino acids with a preference for those having small hydrophobic side chains followed by those bearing polar, aromatic, and basic groups. Does not act on acidic amino acids.1 PublicationManual assertion based on experiment in:Ref.8

Catalytic activity: A D-amino acid + H2O + O2 = a 2-oxo acid + NH3 + H2O2.1 PublicationManual assertion based on experiment in:Ref.7

Cofactor: FAD.
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Research Articles on DAO

1. In the brain, the concentration of D-serine stored in cells is defined by the activity of D-amino acid oxidase.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with DAO elisa kit

 Reference Product  PubMed Publications
 SHMT1 elisa kit  >1 publications with DAO and SHMT1

Pathways associated with DAO elisa kit

 Products by Pathway  Pathway Diagram
 Arginine And Proline Metabolism Pathway antibodies  Arginine And Proline Metabolism Pathway Diagram
 Arginine And Proline Metabolism Pathway antibodies  Arginine And Proline Metabolism Pathway Diagram
 D-Arginine And D-ornithine Metabolism Pathway antibodies  D-Arginine And D-ornithine Metabolism Pathway Diagram
 D-Arginine And D-ornithine Metabolism Pathway antibodies  D-Arginine And D-ornithine Metabolism Pathway Diagram
 Glycine, Serine And Threonine Metabolism Pathway antibodies  Glycine, Serine And Threonine Metabolism Pathway Diagram
 Glycine, Serine And Threonine Metabolism Pathway antibodies  Glycine, Serine And Threonine Metabolism Pathway Diagram
 Glyoxylate Metabolism Pathway antibodies  Glyoxylate Metabolism Pathway Diagram
 Metabolic Pathways antibodies  Metabolic Pathways Diagram
 Metabolism Pathway antibodies  Metabolism Pathway Diagram
 Metabolism Of Amino Acids And Derivatives Pathway antibodies  Metabolism Of Amino Acids And Derivatives Pathway Diagram

Diseases associated with DAO elisa kit

 Disease Name  Pubmed Publications
 Immune System Diseases Antibodies  >180 publications with DAO and Immune System Diseases
 Nervous System Diseases Antibodies  >145 publications with DAO and Nervous System Diseases
 Disease Models, Animal Antibodies  >111 publications with DAO and Disease Models, Animal
 Liver Diseases Antibodies  >109 publications with DAO and Liver Diseases
 Heart Diseases Antibodies  >90 publications with DAO and Heart Diseases
 Inflammation Antibodies  >87 publications with DAO and Inflammation
 Necrosis Antibodies  >79 publications with DAO and Necrosis
 Schizophrenia Antibodies  >73 publications with DAO and Schizophrenia
 Hemorrhage Antibodies  >45 publications with DAO and Hemorrhage
 Hypertension Antibodies  >43 publications with DAO and Hypertension

Organs/Tissues associated with DAO elisa kit

 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >243 publications with DAO and Brain
 Liver Antibodies  >218 publications with DAO and Liver
 Kidney Antibodies  >145 publications with DAO and Kidney
 Muscle Antibodies  >90 publications with DAO and Muscle
 Spinal Cord Antibodies  >27 publications with DAO and Spinal Cord
 Eye Antibodies  >26 publications with DAO and Eye
 Testis Antibodies  >10 publications with DAO and Testis
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