N-Acetyl-Beta-D-Glucosaminidase, NAG (NAG) ELISA Kit for Human

Name: Human NAG ELISA Kit; N-Acetyl-Beta-D-Glucosaminidase, NAG; MyBioSource
Brand: MyBioSource
SKU: MBS700742
Price: 295 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS700742

Principle of the Assay: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antigen. Standards or samples are added to the appropriate microtiter plate wells with… More details

$295 / 24-Strip-Wells (LIMIT 1) +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

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Product Overview

Online Datasheet in PDF

Product Name

N-acetyl-beta-D-glucosaminidase, NAG, ELISA Kit

Full Product Name

Human N-acetyl-beta-D-glucosaminidase, NAG ELISA Kit

Product Gene Name

NAG elisa kit

Product Synonym Names

Human N-acetyl-beta-D-glucosaminidase; NAG ELISA Kit; ENC-1AS; N-acetyl-beta-glucosaminidase; hexosaminidase B; Beta-hexosaminidase subunit beta (HEXB) ; N-acetyl-beta-D-glucosaminidase; NAG

Species Reactivity

Human

Assay Type

Competitive

Samples

Serum, urine

Detection Range

7.81 mIU/ml-2000 mIU/ml

Sensitivity

< 7.81 mIU/ml

Specificity

This assay has high sensitivity and excellent specificity for detection of human NAG. No significant cross-reactivity or interference between human NAG and analogues was observed.

Preparation and Storage

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Inter-assay Precision

Inter-assay Precision (Precision between assays): CV%<15%. Three samples of known concentration were tested in twenty assays to assess.

Intra-assay Precision

Intra-assay Precision (Precision within an assay): CV%<12%. Three samples of known concentration were tested twenty times on one plate to assess.

Molecular Weight

63,111 Da

OMIM

606873

3D Structure

ModBase 3D Structure for P07686

ISO Certification

Manufactured in an ISO 9001:2015 Certified Laboratory.

Other Notes

Small volumes of NAG elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for NAG purchase

MBS700742 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the N-acetyl-beta-D-glucosaminidase, NAG, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing NAG. The ELISA analytical biochemical technique of the MBS700742 kit is based on NAG antibody-NAG antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect NAG antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, NAG. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for NAG. It may not necessarily be applicable to this product.

NCBI GI #

4504373

NCBI GeneID

3074

NCBI Accession #

NP_000512.1

UniProt Primary Accession #

P07686

UniProt Related Accession #

P07686

GenBank Nucleotide #

NM_000521.3

NCBI Official Full Name

beta-hexosaminidase subunit beta isoform 1 preproprotein

NCBI Official Synonym Full Names

hexosaminidase B (beta polypeptide)

NCBI Official Symbol

HEXB

NCBI Official Synonym Symbols

ENC-1AS; HEL-248

UniProt Protein Name

Beta-hexosaminidase subunit beta

UniProt Synonym Protein Names

Beta-N-acetylhexosaminidase subunit beta; Hexosaminidase subunit B; Cervical cancer proto-oncogene 7 protein; HCC-7; N-acetyl-beta-glucosaminidase subunit betaCleaved into the following 2 chains:Beta-hexosaminidase subunit beta chain B; Beta-hexosaminidase subunit beta chain A

UniProt Gene Name

HEXB

UniProt Synonym Gene Names

HCC7; Hexosaminidase subunit B; HCC-7

UniProt Entry Name

HEXB_HUMAN

NCBI Protein Information

beta-hexosaminidase subunit beta; HCC-7; hexosaminidase subunit B; epididymis luminal protein 248; beta-N-acetylhexosaminidase subunit beta; cervical cancer proto-oncogene 7 protein; N-acetyl-beta-glucosaminidase subunit beta

NCBI Summary for NAG

Hexosaminidase B is the beta subunit of the lysosomal enzyme beta-hexosaminidase that, together with the cofactor GM2 activator protein, catalyzes the degradation of the ganglioside GM2, and other molecules containing terminal N-acetyl hexosamines. Beta-hexosaminidase is composed of two subunits, alpha and beta, which are encoded by separate genes. Both beta-hexosaminidase alpha and beta subunits are members of family 20 of glycosyl hydrolases. Mutations in the alpha or beta subunit genes lead to an accumulation of GM2 ganglioside in neurons and neurodegenerative disorders termed the GM2 gangliosidoses. Beta subunit gene mutations lead to Sandhoff disease (GM2-gangliosidosis type II). Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2014]

UniProt Comments for NAG

Function: Responsible for the degradation of GM2 gangliosides, and a variety of other molecules containing terminal N-acetyl hexosamines, in the brain and other tissues.

Catalytic activity: Hydrolysis of terminal non-reducing N-acetyl-D-hexosamine residues in N-acetyl-beta-D-hexosaminides.

Research Articles on NAG

1. A patient with Sandhoff disease also is found to have a compound macro-deletion in HEXB.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with NAG elisa kit

Reference Product	PubMed Publications
HEXA elisa kit	>79 publications with NAG and HEXA
GM2A elisa kit	>9 publications with NAG and GM2A
GLB1 elisa kit	>2 publications with NAG and GLB1
GLA elisa kit	>1 publications with NAG and GLA
NAGA elisa kit	>1 publications with NAG and NAGA

Pathways associated with NAG elisa kit

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Amino Sugar And Nucleotide Sugar Metabolism Pathway antibodies	Amino Sugar And Nucleotide Sugar Metabolism Pathway Diagram
Amino Sugar And Nucleotide Sugar Metabolism Pathway antibodies	Amino Sugar And Nucleotide Sugar Metabolism Pathway Diagram
CS/DS Degradation Pathway antibodies	CS/DS Degradation Pathway Diagram
Chondroitin Sulfate/dermatan Sulfate Metabolism Pathway antibodies	Chondroitin Sulfate/dermatan Sulfate Metabolism Pathway Diagram
Disease Pathway antibodies	Disease Pathway Diagram
Glycogen Storage Diseases Pathway antibodies	Glycogen Storage Diseases Pathway Diagram
Glycosaminoglycan Degradation Pathway antibodies	Glycosaminoglycan Degradation Pathway Diagram
Glycosaminoglycan Degradation Pathway antibodies	Glycosaminoglycan Degradation Pathway Diagram
Glycosaminoglycan Metabolism Pathway antibodies	Glycosaminoglycan Metabolism Pathway Diagram
Glycosphingolipid Biosynthesis - Ganglio Series Pathway antibodies	Glycosphingolipid Biosynthesis - Ganglio Series Pathway Diagram

Diseases associated with NAG elisa kit

Disease Name	Pubmed Publications
Sandhoff Disease Antibodies	>119 publications with NAG and Sandhoff Disease
Brain Diseases Antibodies	>117 publications with NAG and Brain Diseases
Disease Models, Animal Antibodies	>36 publications with NAG and Disease Models, Animal
Liver Diseases Antibodies	>8 publications with NAG and Liver Diseases
Kidney Diseases Antibodies	>7 publications with NAG and Kidney Diseases
Inflammation Antibodies	>4 publications with NAG and Inflammation
Nerve Degeneration Antibodies	>3 publications with NAG and Nerve Degeneration
Hypertrophy Antibodies	>1 publications with NAG and Hypertrophy
Weight Loss Antibodies	>1 publications with NAG and Weight Loss
Seizures Antibodies	>1 publications with NAG and Seizures

Organs/Tissues associated with NAG elisa kit

Organ/Tissue Name	Pubmed Publications
Bone Antibodies	>13 publications with NAG and Bone
Connective Tissue Antibodies	>4 publications with NAG and Connective Tissue
Heart Antibodies	>3 publications with NAG and Heart
Muscle Antibodies	>3 publications with NAG and Muscle
Brain Antibodies	>3 publications with NAG and Brain
Vascular Antibodies	>2 publications with NAG and Vascular
Embryonic Tissue Antibodies	>2 publications with NAG and Embryonic Tissue
Ear Antibodies	>1 publications with NAG and Ear
Kidney Antibodies	>1 publications with NAG and Kidney
Umbilical Cord Antibodies	>1 publications with NAG and Umbilical Cord