Hepcidin 25 (HEPC25) ELISA Kit for Human

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for Hepc has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any Hepc present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for Hepc is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of Hepc bound in the initial step. The color development is stopped and the intensity of the color is measured.

Small volumes of Hepc25 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS700759 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Hepcidin 25 (Hepc25) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing Hepc25. The ELISA analytical biochemical technique of the MBS700759 kit is based on Hepc25 antibody-Hepc25 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect Hepc25 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, Hepc25. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%). Human Hepcidin 25, Hepc25 ELISA Kit.
Hepcidin exists as an 84 amino acid preprohormone a 60 amino acid prohormone (60 amino acids) and as the 25 amino acid hormone (Hepc25). This protein is encoded by the HAMP gene and is a major regulator of the entry of iron into the circulation by reducing iron transport across the gut mucosa. It also reduces iron extrusion from macrophages by reducing the transmembrane iron transporter ferroportin. Hepcidin is synthesized for the most part in the liver. Hepcidin levels tend to be abnormally high under inflammatory conditions and the hepcidin is thought to have anti-inflammatory properties.

The concentration of human hepcidin 25 in a test sample e.g. serum samples can be derived from a standard curve. The details of reagents in this kit can be found in the product datasheet. This use of this assay kit is intended for research purposes.