Nephrosis 1, Congenital, Finnish Type (Nephrin) (NPHS1) ELISA Kit for Human

Introduction: Nephrin is a protein necessary for the proper functioning of therenal filtration barrier. The renal filtration barrier consists offenestrated endothelial cells, the glomerular basementmembrane, and the podocytes of epithelial cells. Nephrin is atransmembrane protein involved with the slit diaphragm. A defectin the gene for nephrin is associated with congenital nephroticsyndrome of the Finnish type and causes massive amounts ofprotein to be leaked into the urine, or proteinuria.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated withan antibody specific to nephrin. Standards or samples are thenadded to the appropriate microtiter plate wells with abiotin-conjugated antibody preparation specific for nephrin andAvidin conjugated to Horseradish Peroxidase (HRP) is added toeach microplate well and incubated. Then a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well.Only those wells that contain nephrin, biotin-conjugated antibodyand enzyme-conjugated Avidin will exhibit a change in color. Theenzyme-substrate reaction is terminated by the addition of asulphuric acid solution and the color change is measuredspectrophotometrically at a wavelength of 450 nm +/- 2 nm. Theconcentration of nephrin in the samples is then determined bycomparing the O.D. of the samples to the standard curve.

Small volumes of NPHS1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS700868 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the nephrosis 1, congenital, Finnish type (nephrin) (NPHS1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing NPHS1. The ELISA analytical biochemical technique of the MBS700868 kit is based on NPHS1 antibody-NPHS1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect NPHS1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, NPHS1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).