L1 Cell Adhesion Molecule (L1CAM) ELISA Kit for Human

Name: Human L1CAM ELISA Kit; L1 Cell Adhesion Molecule; MyBioSource
Brand: MyBioSource
SKU: MBS700933
Price: 295 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS700933

Princple of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for NCAM-L1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any NCAM-L1 present is bound by the… More details

$295 / 24-Strip-Wells (LIMIT 1) +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

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Product Overview

Online Datasheet in PDF

Product Name

L1 cell adhesion molecule (L1CAM), ELISA Kit

Full Product Name

Human Neural cell adhesion molecule ligand 1, NCAM-L1 ELISA Kit

Product Gene Name

L1CAM elisa kit

Product Synonym Names

Human Neural cell adhesion molecule ligand 1; NCAM-L1 ELISA Kit; CAML1; CD171; HSAS; HSAS1; MASA; MIC5; N-CAML1; S10; SPG1; OTTHUMP00000025992; OTTHUMP00000063091; antigen identified by monoclonal antibody R1; neural cell adhesion molecule L1; L1 cell adhesion molecule

Species Reactivity

Human

Assay Type

Sandwich

Samples

Serum, plasma, tissue homogenates

Detection Range

1.25 ng/ml -80 ng/ml.

Sensitivity

The minimum detectable dose of human NCAM-L1 is typically less than 0.31 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.

Specificity

This assay has high sensitivity and excellent specificity for detection of human NCAM-L1. No significant cross-reactivity or interference between human NCAM-L1 and analogues was observed.

Preparation and Storage

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Inter-assay Precision

Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

Intra-assay Precision

Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.

Molecular Weight

140,003 Da

OMIM

308840

3D Structure

ModBase 3D Structure for P32004

ISO Certification

Manufactured in an ISO 9001:2015 Certified Laboratory.

Other Notes

Small volumes of L1CAM elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for L1CAM purchase

MBS700933 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the L1 cell adhesion molecule (L1CAM) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing L1CAM. The ELISA analytical biochemical technique of the MBS700933 kit is based on L1CAM antibody-L1CAM antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect L1CAM antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, L1CAM. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for L1CAM. It may not necessarily be applicable to this product.

NCBI GI #

4557707

NCBI GeneID

3897

NCBI Accession #

NP_000416.1

UniProt Primary Accession #

P32004

UniProt Secondary Accession #

Q8TA87; A0AV65; A4ZYW4; B2RMU7; G3XAF4

UniProt Related Accession #

P32004

GenBank Nucleotide #

NM_000425.4

NCBI Official Full Name

neural cell adhesion molecule L1 isoform 1

NCBI Official Synonym Full Names

L1 cell adhesion molecule

NCBI Official Symbol

L1CAM

NCBI Official Synonym Symbols

S10; HSAS; MASA; MIC5; SPG1; CAML1; CD171; HSAS1; N-CAML1; NCAM-L1; N-CAM-L1

UniProt Protein Name

Neural cell adhesion molecule L1

UniProt Synonym Protein Names

CD_antigen: CD171

Protein Family

Neural cell adhesion molecule

UniProt Gene Name

L1CAM

UniProt Synonym Gene Names

CAML1; MIC5; N-CAM-L1; NCAM-L1

UniProt Entry Name

L1CAM_HUMAN

NCBI Protein Information

neural cell adhesion molecule L1; antigen identified by monoclonal antibody R1

NCBI Summary for L1CAM

The protein encoded by this gene is an axonal glycoprotein belonging to the immunoglobulin supergene family. The ectodomain, consisting of several immunoglobulin-like domains and fibronectin-like repeats (type III), is linked via a single transmembrane sequence to a conserved cytoplasmic domain. This cell adhesion molecule plays an important role in nervous system development, including neuronal migration and differentiation. Mutations in the gene cause X-linked neurological syndromes known as CRASH (corpus callosum hypoplasia, retardation, aphasia, spastic paraplegia and hydrocephalus). Alternative splicing of this gene results in multiple transcript variants, some of which include an alternate exon that is considered to be specific to neurons. [provided by RefSeq, May 2013]

UniProt Comments for L1CAM

Function: Cell adhesion molecule with an important role in the development of the nervous system. Involved in neuron-neuron adhesion, neurite fasciculation, outgrowth of neurites, etc. Binds to axonin on neurons.

Research Articles on L1CAM

1. Human pathological H210Q, R184Q and Y1070C, but not the E309K and L120V L1CAM mutations affect outside-in signaling via the FIGQY Ankyrin binding domain which is required for synapse formation.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with L1CAM elisa kit

Reference Product	PubMed Publications
NCAM1 elisa kit	>1467 publications with L1CAM and NCAM1
CNTN2 elisa kit	>16 publications with L1CAM and CNTN2
NCAN elisa kit	>10 publications with L1CAM and NCAN
ANK2 elisa kit	>7 publications with L1CAM and ANK2
RANBP9 elisa kit	>1 publications with L1CAM and RANBP9
CNTN1 elisa kit	>1 publications with L1CAM and CNTN1
NUMB elisa kit	>1 publications with L1CAM and NUMB

Pathways associated with L1CAM elisa kit

Products by Pathway	Pathway Diagram
Axon Guidance Pathway antibodies	Axon Guidance Pathway Diagram
Axon Guidance Pathway antibodies	Axon Guidance Pathway Diagram
Axon Guidance Pathway antibodies	Axon Guidance Pathway Diagram
Basigin Interactions Pathway antibodies	Basigin Interactions Pathway Diagram
Cell Adhesion Molecules (CAMs) Pathway antibodies	Cell Adhesion Molecules (CAMs) Pathway Diagram
Cell Adhesion Molecules (CAMs) Pathway antibodies	Cell Adhesion Molecules (CAMs) Pathway Diagram
Cell Surface Interactions At The Vascular Wall Pathway antibodies	Cell Surface Interactions At The Vascular Wall Pathway Diagram
Developmental Biology Pathway antibodies	Developmental Biology Pathway Diagram
Hemostasis Pathway antibodies	Hemostasis Pathway Diagram
Interaction Between L1 And Ankyrins Pathway antibodies	Interaction Between L1 And Ankyrins Pathway Diagram

Diseases associated with L1CAM elisa kit

Disease Name	Pubmed Publications
Nervous System Diseases Antibodies	>329 publications with L1CAM and Nervous System Diseases
Brain Diseases Antibodies	>211 publications with L1CAM and Brain Diseases
Disease Models, Animal Antibodies	>83 publications with L1CAM and Disease Models, Animal
Neoplasm Metastasis Antibodies	>51 publications with L1CAM and Neoplasm Metastasis
Adenocarcinoma Antibodies	>49 publications with L1CAM and Adenocarcinoma
Ovarian Neoplasms Antibodies	>33 publications with L1CAM and Ovarian Neoplasms
Cardiovascular Diseases Antibodies	>28 publications with L1CAM and Cardiovascular Diseases
Aphasia Antibodies	>23 publications with L1CAM and Aphasia
Neoplasms, Experimental Antibodies	>22 publications with L1CAM and Neoplasms, Experimental
Hirschsprung Disease Antibodies	>21 publications with L1CAM and Hirschsprung Disease

Organs/Tissues associated with L1CAM elisa kit

Organ/Tissue Name	Pubmed Publications
Brain Antibodies	>735 publications with L1CAM and Brain
Nerve Antibodies	>402 publications with L1CAM and Nerve
Embryonic Tissue Antibodies	>98 publications with L1CAM and Embryonic Tissue
Ganglia Antibodies	>61 publications with L1CAM and Ganglia
Eye Antibodies	>55 publications with L1CAM and Eye
Muscle Antibodies	>45 publications with L1CAM and Muscle
Kidney Antibodies	>35 publications with L1CAM and Kidney
Skin Antibodies	>26 publications with L1CAM and Skin
Lung Antibodies	>23 publications with L1CAM and Lung
Liver Antibodies	>23 publications with L1CAM and Liver