Insulin Autoantibodies, IAA (IAA) ELISA Kit for Human

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with purified insulin antigen. Samples are then added to the appropriate microtiter plate wells and incubated. Then add Horseradish Peroxidase (HRP)-conjugated goat-anti-human antibody to each well and incubate. Finally, substrate solutions are added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. Calculate the valence of insulin autoantibodies in the samples.

Small volumes of IAA elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS701126 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the insulin autoantibodies, IAA, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing IAA. The ELISA analytical biochemical technique of the MBS701126 kit is based on IAA antibody-IAA antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect IAA antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, IAA. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).