Lysozyme C (LZM) (LYZ) ELISA Kit for Chicken

Principle of the Assay: This assay employs the direct competitive inhibition enzyme immunoassay technique. Antibody specific for lysozyme has been pre-coated onto a microplate. Standards and samples are pipetted into the wells with biotin-conjugated lysozyme. A competitive inhibition reaction is launched between lysozyme (Standards or samples) and biotin-conjugated lysozyme with the pre-coated lysozyme antibody. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound reagent, a substrate solution is added to the wells and color develops in opposite to the amount of lysozyme bound in the initial step. The color development is stopped and the intensity of the color is measured.

Small volumes of LYZ elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS701562 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Lysozyme C (LZM) (LYZ) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing LYZ. The ELISA analytical biochemical technique of the MBS701562 kit is based on LYZ antibody-LYZ antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect LYZ antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, LYZ. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).