Keratin 18 (KRT18) ELISA Kit for Human

Name: Human KRT18 ELISA Kit; Keratin 18; MyBioSource
Brand: MyBioSource
SKU: MBS702127
Price: 295 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS702127

Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for CK-18 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells with a Horseradish Peroxidase (HRP)… More details

$295 / 24-Strip-Wells (LIMIT 1) +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

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Product Overview

Online Datasheet in PDF

Product Name

keratin 18 (KRT18), ELISA Kit

Full Product Name

Human cytokeratin 18, CK-18 ELISA Kit

Product Gene Name

KRT18 elisa kit

Product Synonym Names

Human cytokeratin 18; CK-18 ELISA Kit; CYK18; K18; cell proliferation-inducing protein 46; cytokeratin 18; keratin 18

Species Reactivity

Human

Assay Type

Sandwich

Samples

Serum, plasma, tissue homogenates, Cell lysates, cell culture supernates

Detection Range

0.625 ng/ml-15 ng/ml.

Sensitivity

The minimum detectable dose of human CK-18 is typically less than 0.25 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest human CK-18 concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.

Specificity

This assay has high sensitivity and excellent specificity for detection of human CK-18. No significant cross-reactivity or interference between human CK-18 and analogues was observed.

Preparation and Storage

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Inter-assay Precision

Inter-assay Precision (Precision between assays): CV%<15%. Three samples of known concentration were tested in twenty assays to assess.

Intra-assay Precision

Intra-assay Precision (Precision within an assay): CV%<10%. Three samples of known concentration were tested twenty times on one plate to assess.

Molecular Weight

48,058 Da

OMIM

gene 215600

3D Structure

ModBase 3D Structure for P05783

ISO Certification

Manufactured in an ISO 9001:2015 Certified Laboratory.

Other Notes

Small volumes of KRT18 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for KRT18 purchase

MBS702127 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the keratin 18 (KRT18) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing KRT18. The ELISA analytical biochemical technique of the MBS702127 kit is based on KRT18 antibody-KRT18 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect KRT18 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, KRT18. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for KRT18. It may not necessarily be applicable to this product.

NCBI GI #

4557888

NCBI GeneID

3875

NCBI Accession #

NP_000215.1

UniProt Primary Accession #

P05783

UniProt Secondary Accession #

Q53G38; Q5U0N8; Q9BW26

UniProt Related Accession #

P05783

GenBank Nucleotide #

NM_000224.2

NCBI Official Full Name

keratin, type I cytoskeletal 18

NCBI Official Synonym Full Names

keratin 18

NCBI Official Symbol

KRT18

NCBI Official Synonym Symbols

K18; CYK18

UniProt Protein Name

Keratin, type I cytoskeletal 18

UniProt Synonym Protein Names

Cell proliferation-inducing gene 46 protein; Cytokeratin-18; CK-18; Keratin-18; K18

Protein Family

Keratin

UniProt Gene Name

KRT18

UniProt Synonym Gene Names

CYK18; PIG46; CK-18; K18

UniProt Entry Name

K1C18_HUMAN

NCBI Protein Information

keratin, type I cytoskeletal 18; CK-18; keratin-18; cytokeratin 18; cytokeratin-18; cell proliferation-inducing protein 46; cell proliferation-inducing gene 46 protein

NCBI Summary for KRT18

KRT18 encodes the type I intermediate filament chain keratin 18. Keratin 18, together with its filament partner keratin 8, are perhaps the most commonly found members of the intermediate filament gene family. They are expressed in single layer epithelial tissues of the body. Mutations in this gene have been linked to cryptogenic cirrhosis. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008]

UniProt Comments for KRT18

Function: Involved in the uptake of thrombin-antithrombin complexes by hepatic cells When phosphorylated, plays a role in filament reorganization. Involved in the delivery of mutated CFTR to the plasma membrane. Together with KRT8, is involved in interleukin-6 (IL-6)-mediated barrier protection.7 PublicationsManual assertion based on experiment in:Ref.11

Research Articles on KRT18

1. parkin may inhibit cell growth, at least in part, by decreasing RPSA expression and inducing phosphorylation of cytokeratin 8/18

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with KRT18 elisa kit

Reference Product	PubMed Publications
KRT8 elisa kit	>47 publications with KRT18 and KRT8
TCHP elisa kit	>1 publications with KRT18 and TCHP
CASP6 elisa kit	>1 publications with KRT18 and CASP6

Pathways associated with KRT18 elisa kit

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Pathogenic Escherichia Coli Infection Pathway antibodies	Pathogenic Escherichia Coli Infection Pathway Diagram
Pathogenic Escherichia Coli Infection Pathway antibodies	Pathogenic Escherichia Coli Infection Pathway Diagram
Pathogenic Escherichia Coli Infection Pathway antibodies	Pathogenic Escherichia Coli Infection Pathway Diagram

Diseases associated with KRT18 elisa kit

Disease Name	Pubmed Publications
Liver Diseases Antibodies	>30 publications with KRT18 and Liver Diseases
Carcinoma Antibodies	>28 publications with KRT18 and Carcinoma
Breast Neoplasms Antibodies	>15 publications with KRT18 and Breast Neoplasms
Adenocarcinoma Antibodies	>14 publications with KRT18 and Adenocarcinoma
Fibrosis Antibodies	>13 publications with KRT18 and Fibrosis
Neoplasm Metastasis Antibodies	>11 publications with KRT18 and Neoplasm Metastasis
Fatty Liver Antibodies	>11 publications with KRT18 and Fatty Liver
Necrosis Antibodies	>9 publications with KRT18 and Necrosis
Liver Cirrhosis Antibodies	>8 publications with KRT18 and Liver Cirrhosis
Liver Neoplasms Antibodies	>7 publications with KRT18 and Liver Neoplasms

Organs/Tissues associated with KRT18 elisa kit

Organ/Tissue Name	Pubmed Publications
Liver Antibodies	>26 publications with KRT18 and Liver
Blood Antibodies	>15 publications with KRT18 and Blood
Kidney Antibodies	>6 publications with KRT18 and Kidney
Prostate Antibodies	>5 publications with KRT18 and Prostate
Muscle Antibodies	>5 publications with KRT18 and Muscle
Eye Antibodies	>4 publications with KRT18 and Eye
Vascular Antibodies	>4 publications with KRT18 and Vascular
Lymph Antibodies	>4 publications with KRT18 and Lymph
Lymph Node Antibodies	>4 publications with KRT18 and Lymph Node
Bladder Antibodies	>4 publications with KRT18 and Bladder