Glutamate Decarboxylase 2 (Pancreatic Islets and Brain, 65kDa) (GAD2) ELISA Kit for Human

Name: Human GAD2 ELISA Kit; MyBioSource
Brand: MyBioSource
SKU: MBS702132
Price: 295 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS702132

MBS702132 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the glutamate decarboxylase 2 (pancreatic islets and brain, 65kDa) (GAD2) ELISA Kit target analytes in biological samples. The… More details

$295 / 24-Strip-Wells (LIMIT 1) +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

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Product Overview

Online Datasheet in PDF

Product Name

glutamate decarboxylase 2 (pancreatic islets and brain, 65kDa) (GAD2), ELISA Kit

Full Product Name

Human glutamic acid decarboxylase 65, GAD65 ELISA Kit

Product Gene Name

GAD2 elisa kit

Product Synonym Names

Human glutamic acid decarboxylase 65; GAD65 ELISA Kit; RP11-420F12.2; GAD65; MGC161605; MGC161607; Glutamate decarboxylase-2 (pancreas) ; glutamate decarboxylase 2; glutamate decarboxylase 2 (pancreatic islets and brain; 65kDa)

Species Reactivity

Human

Assay Type

Sandwich (Quantitative)

Samples

serum, plasma, tissue homogenates.

Detection Range

0.156 ng/ml-10 ng/ml.

Sensitivity

Typically less than 0.039 ng/ml

Specificity

This assay has high sensitivity and excellent specificity for detection of human GAD65. No significant cross-reactivity or interference between human GAD65 and analogues was observed.

Preparation and Storage

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Inter-assay Precision

Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

Intra-assay Precision

Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.

Molecular Weight

65,411 Da

OMIM

138275

3D Structure

ModBase 3D Structure for Q05329

ISO Certification

Manufactured in an ISO 9001:2015 Certified Laboratory.

Other Notes

Small volumes of GAD2 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for GAD2. It may not necessarily be applicable to this product.

NCBI GI #

4503875

NCBI GeneID

2572

NCBI Accession #

NP_000809.1

UniProt Primary Accession #

Q05329

UniProt Secondary Accession #

Q9UD87

UniProt Related Accession #

Q05329

GenBank Nucleotide #

NM_000818.2

NCBI Official Full Name

glutamate decarboxylase 2

NCBI Official Synonym Full Names

glutamate decarboxylase 2 (pancreatic islets and brain, 65kDa)

NCBI Official Symbol

GAD2

NCBI Official Synonym Symbols

GAD65

UniProt Protein Name

Glutamate decarboxylase 2

UniProt Synonym Protein Names

65 kDa glutamic acid decarboxylase; GAD-65; Glutamate decarboxylase 65 kDa isoform

Protein Family

Glutamate decarboxylase

UniProt Gene Name

GAD2

UniProt Synonym Gene Names

GAD65; GAD-65

UniProt Entry Name

DCE2_HUMAN

NCBI Protein Information

glutamate decarboxylase 2; GAD-65; 65 kDa glutamic acid decarboxylase; Glutamate decarboxylase-2 (pancreas); glutamate decarboxylase 65 kDa isoform

NCBI Summary for GAD2

This gene encodes one of several forms of glutamic acid decarboxylase, identified as a major autoantigen in insulin-dependent diabetes. The enzyme encoded is responsible for catalyzing the production of gamma-aminobutyric acid from L-glutamic acid. A pathogenic role for this enzyme has been identified in the human pancreas since it has been identified as an autoantibody and an autoreactive T cell target in insulin-dependent diabetes. This gene may also play a role in the stiff man syndrome. Alternative splicing results in multiple transcript variants that encode the same protein. [provided by RefSeq, Oct 2008]

UniProt Comments for GAD2

Function: Catalyzes the production of GABA.

Catalytic activity: L-glutamate = 4-aminobutanoate + CO2.

Cofactor: Pyridoxal phosphate.1 PublicationManual assertion based on experiment in:Ref.10

Research Articles on GAD2

1. GAD65 expression in 313 gastric cancer tissues was significantly higher than in 60 adjacent non-tumour tissues. Moreover, the expression level of GAD65 significantly correlated with the depth of tumour invasion and TNM stage.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with GAD2 elisa kit

Reference Product	PubMed Publications
GAD1 elisa kit	>48 publications with GAD2 and GAD1
GLUL elisa kit	>1 publications with GAD2 and GLUL
INS elisa kit	>1 publications with GAD2 and INS
SLC32A1 elisa kit	>1 publications with GAD2 and SLC32A1
ABAT elisa kit	>1 publications with GAD2 and ABAT

Pathways associated with GAD2 elisa kit

Products by Pathway	Pathway Diagram
Alanine And Aspartate Metabolism Pathway antibodies	Alanine And Aspartate Metabolism Pathway Diagram
Alanine, Aspartate And Glutamate Metabolism Pathway antibodies	Alanine, Aspartate And Glutamate Metabolism Pathway Diagram
Alanine, Aspartate And Glutamate Metabolism Pathway antibodies	Alanine, Aspartate And Glutamate Metabolism Pathway Diagram
Biogenic Amine Synthesis Pathway antibodies	Biogenic Amine Synthesis Pathway Diagram
Butanoate Metabolism Pathway antibodies	Butanoate Metabolism Pathway Diagram
Butanoate Metabolism Pathway antibodies	Butanoate Metabolism Pathway Diagram
GABA (gamma-Aminobutyrate) Shunt Pathway antibodies	GABA (gamma-Aminobutyrate) Shunt Pathway Diagram
GABA (gamma-Aminobutyrate) Shunt Pathway antibodies	GABA (gamma-Aminobutyrate) Shunt Pathway Diagram
GABA Synthesis Pathway antibodies	GABA Synthesis Pathway Diagram
GABA Synthesis, Release, Reuptake And Degradation Pathway antibodies	GABA Synthesis, Release, Reuptake And Degradation Pathway Diagram

Diseases associated with GAD2 elisa kit

Disease Name	Pubmed Publications
Mental Disorders Antibodies	>18 publications with GAD2 and Mental Disorders
Nervous System Diseases Antibodies	>12 publications with GAD2 and Nervous System Diseases
Brain Diseases Antibodies	>10 publications with GAD2 and Brain Diseases
Schizophrenia Antibodies	>9 publications with GAD2 and Schizophrenia
Epilepsy Antibodies	>6 publications with GAD2 and Epilepsy
Seizures Antibodies	>6 publications with GAD2 and Seizures
Inflammation Antibodies	>4 publications with GAD2 and Inflammation
Depressive Disorder Antibodies	>3 publications with GAD2 and Depressive Disorder
Movement Disorders Antibodies	>2 publications with GAD2 and Movement Disorders
Substance Withdrawal Syndrome Antibodies	>2 publications with GAD2 and Substance Withdrawal Syndrome

Organs/Tissues associated with GAD2 elisa kit

Organ/Tissue Name	Pubmed Publications
Brain Antibodies	>22 publications with GAD2 and Brain
Pancreas Antibodies	>2 publications with GAD2 and Pancreas