Integrin, Alpha M (Complement Component 3 Receptor 3 Subunit) (ITGAM) ELISA Kit for Human

Name: Human ITGAM ELISA Kit; MyBioSource
Brand: MyBioSource
SKU: MBS702254
Price: 295 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS702254

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for ITG/CD11b has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any ITG/CD11b present is bound by… More details

$295 / 24-Strip-Wells (LIMIT 1) +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

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Product Overview

Online Datasheet in PDF

Product Name

integrin, alpha M (complement component 3 receptor 3 subunit) (ITGAM), ELISA Kit

Full Product Name

Human Integrin alphaM, ITG/CD11b ELISA Kit

Product Gene Name

ITGAM elisa kit

Product Synonym Names

Human Integrin alphaM; ITG/CD11b ELISA Kit; CD11B; CR3A; MAC-1; MAC1A; MGC117044; MO1A; SLEB6; antigen CD11b (p170) ; integrin alpha M; macrophage antigen alpha polypeptide; neutrophil adherence receptor alpha-M subunit; integrin; alpha M (complement component 3 receptor 3 subunit)

Species Reactivity

Human

Assay Type

Quantitative Sandwich

Samples

Serum, plasma and tissue homogenates

Detection Range

0.78 ng/ml-50 ng/ml

Sensitivity

< 0.195 ng/ml

Specificity

This assay has high sensitivity and excellent specificity for detection of human ITG/CD11b. No significant cross-reactivity or interference between human ITG/CD11b and analogues was observed.

Preparation and Storage

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Inter-assay Precision

Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

Intra-assay Precision

Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.

Molecular Weight

127,179 Da

OMIM

gene 609939

3D Structure

ModBase 3D Structure for P11215

ISO Certification

Manufactured in an ISO 9001:2015 Certified Laboratory.

Other Notes

Small volumes of ITGAM elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for ITGAM purchase

MBS702254 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the integrin, alpha M (complement component 3 receptor 3 subunit) (ITGAM) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing ITGAM. The ELISA analytical biochemical technique of the MBS702254 kit is based on ITGAM antibody-ITGAM antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect ITGAM antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, ITGAM. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for ITGAM. It may not necessarily be applicable to this product.

NCBI GI #

88501734

NCBI GeneID

3684

NCBI Accession #

NP_000623.2

UniProt Primary Accession #

P11215

UniProt Secondary Accession #

Q4VAK0; Q4VAK1; Q4VAK2

UniProt Related Accession #

P11215

GenBank Nucleotide #

NM_000632.3

NCBI Official Full Name

integrin alpha-M isoform 2

NCBI Official Synonym Full Names

integrin, alpha M (complement component 3 receptor 3 subunit)

NCBI Official Symbol

ITGAM

NCBI Official Synonym Symbols

CR3A; MO1A; CD11B; MAC-1; MAC1A; SLEB6

UniProt Protein Name

Integrin alpha-M

UniProt Synonym Protein Names

CD11 antigen-like family member B; CR-3 alpha chain; Cell surface glycoprotein MAC-1 subunit alpha; Leukocyte adhesion receptor MO1; Neutrophil adherence receptor; CD_antigen: CD11b

Protein Family

Integrin

UniProt Gene Name

ITGAM

UniProt Synonym Gene Names

CD11B; CR3A

UniProt Entry Name

ITAM_HUMAN

NCBI Protein Information

integrin alpha-M; CR-3 alpha chain; antigen CD11b (p170); leukocyte adhesion receptor MO1; CD11 antigen-like family member B; macrophage antigen alpha polypeptide; cell surface glycoprotein MAC-1 subunit alpha; neutrophil adherence receptor alpha-M subunit

NCBI Summary for ITGAM

This gene encodes the integrin alpha M chain. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. This I-domain containing alpha integrin combines with the beta 2 chain (ITGB2) to form a leukocyte-specific integrin referred to as macrophage receptor 1 ('Mac-1'), or inactivated-C3b (iC3b) receptor 3 ('CR3'). The alpha M beta 2 integrin is important in the adherence of neutrophils and monocytes to stimulated endothelium, and also in the phagocytosis of complement coated particles. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2009]

UniProt Comments for ITGAM

Function: Integrin alpha-M/beta-2 is implicated in various adhesive interactions of monocytes, macrophages and granulocytes as well as in mediating the uptake of complement-coated particles. It is identical with CR-3, the receptor for the iC3b fragment of the third complement component. It probably recognizes the R-G-D peptide in C3b. Integrin alpha-M/beta-2 is also a receptor for fibrinogen, factor X and ICAM1. It recognizes P1 and P2 peptides of fibrinogen gamma chain.

Research Articles on ITGAM

1. genetic polymorphism is associated with systemic lupus erythematosus in Brazilian patients

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with ITGAM elisa kit

Reference Product	PubMed Publications
ITGB2 elisa kit	>1634 publications with ITGAM and ITGB2
ICAM1 elisa kit	>436 publications with ITGAM and ICAM1
ICAM2 elisa kit	>20 publications with ITGAM and ICAM2
PLAUR elisa kit	>14 publications with ITGAM and PLAUR
THY1 elisa kit	>14 publications with ITGAM and THY1
ICAM4 elisa kit	>6 publications with ITGAM and ICAM4
SELP elisa kit	>6 publications with ITGAM and SELP
JAM3 elisa kit	>5 publications with ITGAM and JAM3

Pathways associated with ITGAM elisa kit

Products by Pathway	Pathway Diagram
Amoebiasis Pathway antibodies	Amoebiasis Pathway Diagram
Amoebiasis Pathway antibodies	Amoebiasis Pathway Diagram
Cell Adhesion Molecules (CAMs) Pathway antibodies	Cell Adhesion Molecules (CAMs) Pathway Diagram
Cell Adhesion Molecules (CAMs) Pathway antibodies	Cell Adhesion Molecules (CAMs) Pathway Diagram
Cell Surface Interactions At The Vascular Wall Pathway antibodies	Cell Surface Interactions At The Vascular Wall Pathway Diagram
Extracellular Matrix Organization Pathway antibodies	Extracellular Matrix Organization Pathway Diagram
Focal Adhesion Pathway antibodies	Focal Adhesion Pathway Diagram
Hematopoietic Cell Lineage Pathway antibodies	Hematopoietic Cell Lineage Pathway Diagram
Hematopoietic Cell Lineage Pathway antibodies	Hematopoietic Cell Lineage Pathway Diagram
Hemostasis Pathway antibodies	Hemostasis Pathway Diagram

Diseases associated with ITGAM elisa kit

Disease Name	Pubmed Publications
Inflammation Antibodies	>1357 publications with ITGAM and Inflammation
Necrosis Antibodies	>806 publications with ITGAM and Necrosis
Disease Models, Animal Antibodies	>785 publications with ITGAM and Disease Models, Animal
Nervous System Diseases Antibodies	>610 publications with ITGAM and Nervous System Diseases
Cardiovascular Diseases Antibodies	>489 publications with ITGAM and Cardiovascular Diseases
Brain Diseases Antibodies	>416 publications with ITGAM and Brain Diseases
Neoplasms, Experimental Antibodies	>175 publications with ITGAM and Neoplasms, Experimental
Heart Diseases Antibodies	>152 publications with ITGAM and Heart Diseases
Pain Antibodies	>122 publications with ITGAM and Pain
Atherosclerosis Antibodies	>119 publications with ITGAM and Atherosclerosis

Organs/Tissues associated with ITGAM elisa kit

Organ/Tissue Name	Pubmed Publications
Blood Antibodies	>3890 publications with ITGAM and Blood
Bone Antibodies	>682 publications with ITGAM and Bone
Bone Marrow Antibodies	>634 publications with ITGAM and Bone Marrow
Brain Antibodies	>445 publications with ITGAM and Brain
Lung Antibodies	>407 publications with ITGAM and Lung
Spleen Antibodies	>398 publications with ITGAM and Spleen
Lymph Node Antibodies	>232 publications with ITGAM and Lymph Node
Nerve Antibodies	>174 publications with ITGAM and Nerve
Intestine Antibodies	>125 publications with ITGAM and Intestine
Kidney Antibodies	>113 publications with ITGAM and Kidney