Matrix Metallopeptidase 13 (Collagenase 3) (MMP13) ELISA Kit for Human

Name: Human MMP13 ELISA Kit; Matrix Metallopeptidase 13 (Collagenase 3); MyBioSource
Brand: MyBioSource
SKU: MBS702282
Price: 295 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS702282

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for MMP-13 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any MMP-13 present is bound by the… More details

$295 / 24-Strip-Wells (LIMIT 1) +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

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Product Overview

Online Datasheet in PDF

Product Name

matrix metallopeptidase 13 (collagenase 3) (MMP13), ELISA Kit

Full Product Name

Human Matrix metalloproteinase 13, MMP-13 ELISA Kit

Product Gene Name

MMP13 elisa kit

Product Synonym Names

Human Matrix metalloproteinase 13; MMP-13 ELISA kit; CLG3; MANDP1; collagenase 3; matrix metalloproteinase 13; matrix metalloproteinase 13 (collagenase 3) ; matrix metallopeptidase 13 (collagenase 3)

Species Reactivity

Human

Assay Type

Sandwich

Samples

Serum, cell culture supernates, urine, tissue homogenates

Detection Range

0.156 ng/ml-10 ng/ml

Sensitivity

0.039 ng/ml

Specificity

This assay has high sensitivity and excellent specificity for detection of Human MMP13. No significant cross-reactivity or interference between Human MMP13 and analogues was observed.

Preparation and Storage

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Intra-assay Precision

Intra-assay Precision (Precision within an assay): CV% is less than 8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV% is less than 10%
Three samples of known concentration were tested in twenty assays to assess.

Molecular Weight

53,820 Da

OMIM

gene 602111

3D Structure

ModBase 3D Structure for P45452

Detection Wavelength

450 nm

Sample Volume

50-100ul

Protein Biological Process 1

Developmental Protein

Protein Biological Process 3

Collagen degradation

ISO Certification

Manufactured in an ISO 9001:2015 Certified Laboratory.

Other Notes

Small volumes of MMP13 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for MMP13 purchase

MBS702282 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the matrix metallopeptidase 13 (collagenase 3) (MMP13) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing MMP13. The ELISA analytical biochemical technique of the MBS702282 kit is based on MMP13 antibody-MMP13 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect MMP13 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, MMP13. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for MMP13. It may not necessarily be applicable to this product.

NCBI GI #

4505209

NCBI GeneID

4322

NCBI Accession #

NP_002418.1

UniProt Primary Accession #

P45452

UniProt Secondary Accession #

Q6NWN6; A8K846; B2RCZ3

UniProt Related Accession #

P45452

GenBank Nucleotide #

NM_002427.3

NCBI Official Full Name

collagenase 3 preproprotein

NCBI Official Synonym Full Names

matrix metallopeptidase 13 (collagenase 3)

NCBI Official Symbol

MMP13

NCBI Official Synonym Symbols

CLG3; MANDP1; MMP-13

UniProt Protein Name

Collagenase 3

UniProt Synonym Protein Names

Matrix metalloproteinase-13; MMP-13

Protein Family

Collagenase

UniProt Gene Name

MMP13

UniProt Synonym Gene Names

MMP-13

UniProt Entry Name

MMP13_HUMAN

NCBI Protein Information

collagenase 3; matrix metalloproteinase 13 (collagenase 3)

NCBI Summary for MMP13

Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. The protein encoded by this gene cleaves type II collagen more efficiently than types I and III. It may be involved in articular cartilage turnover and cartilage pathophysiology associated with osteoarthritis. The gene is part of a cluster of MMP genes which localize to chromosome 11q22.3. [provided by RefSeq, Jul 2008]

UniProt Comments for MMP13

Function: Plays a role in the degradation of extracellular matrix proteins including fibrillar collagen, fibronectin, TNC and ACAN. Cleaves triple helical collagens, including type I, type II and type III collagen, but has the highest activity with soluble type II collagen. Can also degrade collagen type IV, type XIV and type X. May also function by activating or degrading key regulatory proteins, such as TGFB1 and CTGF. Plays a role in wound healing, tissue remodeling, cartilage degradation, bone development, bone mineralization and ossification. Required for normal embryonic bone development and ossification. Plays a role in the healing of bone fractures via endochondral ossification. Plays a role in wound healing, probably by a mechanism that involves proteolytic activation of TGFB1 and degradation of CTGF. Plays a role in keratinocyte migration during wound healing. May play a role in cell migration and in tumor cell invasion.12 PublicationsManual assertion based on experiment in:Ref.1

Cofactor: Calcium. Can bind about 5 calcium ions per subunit.9 PublicationsManual assertion based on experiment in:Ref.16

Enzyme regulation: Inhibited by TIMP1, TIMP2 and TIMP3. Inhibited by acetohydroxamic acid and other zinc chelators.3 PublicationsManual assertion based on experiment in:Ref.6

Research Articles on MMP13

1. In colorectal adenoma-carcinoma sequence, MMP- 13 overexpression occurred early in the adenoma stage and persisted after malignant transformation.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with MMP13 elisa kit

Reference Product	PubMed Publications
MMP3 elisa kit	>120 publications with MMP13 and MMP3
RUNX2 elisa kit	>59 publications with MMP13 and RUNX2
MMP14 elisa kit	>50 publications with MMP13 and MMP14
ADAMTS5 elisa kit	>37 publications with MMP13 and ADAMTS5
IL6 elisa kit	>23 publications with MMP13 and IL6
IL1B elisa kit	>17 publications with MMP13 and IL1B
COL18A1 elisa kit	>2 publications with MMP13 and COL18A1
PLG elisa kit	>1 publications with MMP13 and PLG

Pathways associated with MMP13 elisa kit

Products by Pathway	Pathway Diagram
AGE/RAGE Pathway antibodies	AGE/RAGE Pathway Diagram
Activation Of Matrix Metalloproteinases Pathway antibodies	Activation Of Matrix Metalloproteinases Pathway Diagram
Assembly Of Collagen Fibrils And Other Multimeric Structures Pathway antibodies	Assembly Of Collagen Fibrils And Other Multimeric Structures Pathway Diagram
Collagen Degradation Pathway antibodies	Collagen Degradation Pathway Diagram
Collagen Formation Pathway antibodies	Collagen Formation Pathway Diagram
Degradation Of The Extracellular Matrix Pathway antibodies	Degradation Of The Extracellular Matrix Pathway Diagram
Endochondral Ossification Pathway antibodies	Endochondral Ossification Pathway Diagram
Extracellular Matrix Organization Pathway antibodies	Extracellular Matrix Organization Pathway Diagram
Matrix Metalloproteinases Pathway antibodies	Matrix Metalloproteinases Pathway Diagram
Oncostatin M Signaling Pathway antibodies	Oncostatin M Signaling Pathway Diagram

Diseases associated with MMP13 elisa kit

Disease Name	Pubmed Publications
Inflammation Antibodies	>173 publications with MMP13 and Inflammation
Disease Models, Animal Antibodies	>149 publications with MMP13 and Disease Models, Animal
Necrosis Antibodies	>147 publications with MMP13 and Necrosis
Fibrosis Antibodies	>95 publications with MMP13 and Fibrosis
Hypertrophy Antibodies	>63 publications with MMP13 and Hypertrophy
Liver Diseases Antibodies	>60 publications with MMP13 and Liver Diseases
Neoplasm Metastasis Antibodies	>52 publications with MMP13 and Neoplasm Metastasis
Breast Neoplasms Antibodies	>46 publications with MMP13 and Breast Neoplasms
Lung Diseases Antibodies	>34 publications with MMP13 and Lung Diseases
Heart Diseases Antibodies	>28 publications with MMP13 and Heart Diseases

Organs/Tissues associated with MMP13 elisa kit

Organ/Tissue Name	Pubmed Publications
Bone Antibodies	>13 publications with MMP13 and Bone
Connective Tissue Antibodies	>4 publications with MMP13 and Connective Tissue
Heart Antibodies	>3 publications with MMP13 and Heart
Muscle Antibodies	>3 publications with MMP13 and Muscle
Brain Antibodies	>3 publications with MMP13 and Brain
Vascular Antibodies	>2 publications with MMP13 and Vascular
Embryonic Tissue Antibodies	>2 publications with MMP13 and Embryonic Tissue
Ear Antibodies	>1 publications with MMP13 and Ear
Kidney Antibodies	>1 publications with MMP13 and Kidney
Umbilical Cord Antibodies	>1 publications with MMP13 and Umbilical Cord