Heat Shock 70kDa Protein 1a (HSPA1A) ELISA Kit for Mouse

Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for Hsp-70 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any Hsp-70 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for Hsp-70 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of Hsp-70 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Small volumes of HSPA1A elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS702702 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the heat shock 70kDa protein 1A (HSPA1A) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing HSPA1A. The ELISA analytical biochemical technique of the MBS702702 kit is based on HSPA1A antibody-HSPA1A antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect HSPA1A antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, HSPA1A. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).