Caspase-9, Casp-9 (CASP-9) ELISA Kit for Rat

Name: Rat CASP-9 ELISA Kit; Caspase-9, Casp-9; MyBioSource
Brand: MyBioSource
SKU: MBS702728
Price: 295 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS702728

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for CALR has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any CALR present is bound by the… More details

$295 / 24-Strip-Wells (LIMIT 1) +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

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Product Overview

Online Datasheet in PDF

Product Name

Caspase-9, Casp-9, ELISA Kit

Full Product Name

Rat Caspase-9, Casp-9 ELISA Kit

Product Gene Name

Casp-9 elisa kit

Product Synonym Names

Rat Caspase-9; Casp-9 ELISA Kit; Caspase-9; Casp-9

Species Reactivity

Rat

Assay Type

Quantitative Sandwich

Samples

Serum, Plasma, Tissue Homogenates

Detection Range

0.78 ng/ml-50 ng/ml

Sensitivity

0.195 ng/ml.

Specificity

This assay has high sensitivity and excellent specificity for detection of mouse CALR. No significant cross-reactivity or interference between mouse CALR and analogues was observed.

Preparation and Storage

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Inter-assay Precision

Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

Intra-assay Precision

Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess

Molecular Weight

36,564 Da

OMIM

602234

3D Structure

ModBase 3D Structure for P55211

ISO Certification

Manufactured in an ISO 9001:2015 Certified Laboratory.

Other Notes

Small volumes of Casp-9 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for Casp-9 purchase

MBS702728 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Caspase-9, Casp-9, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing Casp-9. The ELISA analytical biochemical technique of the MBS702728 kit is based on Casp-9 antibody-Casp-9 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect Casp-9 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, Casp-9. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for Casp-9. It may not necessarily be applicable to this product.

NCBI GI #

14790124

NCBI GeneID

842

NCBI Accession #

NP_001220.2

UniProt Primary Accession #

P55211

UniProt Secondary Accession #

O95348; Q53Y70; Q5JRU9; Q5UGI1; Q92852; Q9BQ62; Q9UEQ3; Q9UIJ8; B4E1A3

UniProt Related Accession #

P55211

GenBank Nucleotide #

NM_001229.4

NCBI Official Full Name

caspase-9 isoform alpha

NCBI Official Synonym Full Names

caspase 9, apoptosis-related cysteine peptidase

NCBI Official Symbol

CASP9

NCBI Official Synonym Symbols

MCH6; APAF3; APAF-3; PPP1R56; ICE-LAP6

UniProt Protein Name

Caspase-9

UniProt Synonym Protein Names

Apoptotic protease Mch-6; Apoptotic protease-activating factor 3; APAF-3; ICE-like apoptotic protease 6; ICE-LAP6

Protein Family

Caspase

UniProt Gene Name

CASP9

UniProt Synonym Gene Names

MCH6; CASP-9; APAF-3; ICE-LAP6

UniProt Entry Name

CASP9_HUMAN

NCBI Protein Information

caspase-9; apoptotic protease MCH-6; ICE-like apoptotic protease 6; apoptotic protease activating factor 3; protein phosphatase 1, regulatory subunit 56

NCBI Summary for Casp-9

This gene encodes a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. This protein can undergo autoproteolytic processing and activation by the apoptosome, a protein complex of cytochrome c and the apoptotic peptidase activating factor 1; this step is thought to be one of the earliest in the caspase activation cascade. This protein is thought to play a central role in apoptosis and to be a tumor suppressor. Alternative splicing results in multiple transcript variants. [provided by RefSeq, May 2013]

UniProt Comments for Casp-9

Function: Involved in the activation cascade of caspases responsible for apoptosis execution. Binding of caspase-9 to Apaf-1 leads to activation of the protease which then cleaves and activates caspase-3. Promotes DNA damage-induced apoptosis in a ABL1/c-Abl-dependent manner. Proteolytically cleaves poly(ADP-ribose) polymerase (PARP).2 PublicationsManual assertion based on experiment in:Ref.17

Catalytic activity: Strict requirement for an Asp residue at position P1 and with a marked preference for His at position P2. It has a preferred cleavage sequence of Leu-Gly-His-Asp-|-Xaa.1 PublicationManual assertion based on experiment in:Ref.24

Enzyme regulation: Inhibited by the effector protein NleF that is produced by pathogenic E.coli; this inhibits apoptosis.1 PublicationManual assertion based on experiment in:Ref.24

Research Articles on Casp-9

1. that KAT5 RNAi may result in cleaved casp9 upregulation through p38MAPK activation in Gallbladder carcinoma cells

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with Casp-9 elisa kit

Reference Product	PubMed Publications
CASP3 elisa kit	>5770 publications with Casp-9 and CASP3
BCL2L1 elisa kit	>534 publications with Casp-9 and BCL2L1
APAF1 elisa kit	>411 publications with Casp-9 and APAF1
XIAP elisa kit	>404 publications with Casp-9 and XIAP
DIABLO elisa kit	>255 publications with Casp-9 and DIABLO
CASP1 elisa kit	>178 publications with Casp-9 and CASP1
AKT1 elisa kit	>130 publications with Casp-9 and AKT1
BIRC3 elisa kit	>18 publications with Casp-9 and BIRC3
BIRC2 elisa kit	>16 publications with Casp-9 and BIRC2
DCC elisa kit	>4 publications with Casp-9 and DCC

Pathways associated with Casp-9 elisa kit

Products by Pathway	Pathway Diagram
AGE/RAGE Pathway antibodies	AGE/RAGE Pathway Diagram
AKT Phosphorylates Targets In The Cytosol Pathway antibodies	AKT Phosphorylates Targets In The Cytosol Pathway Diagram
Activation Of Caspases Through Apoptosome-mediated Cleavage Pathway antibodies	Activation Of Caspases Through Apoptosome-mediated Cleavage Pathway Diagram
Adaptive Immune System Pathway antibodies	Adaptive Immune System Pathway Diagram
Alzheimer's Disease Pathway antibodies	Alzheimer's Disease Pathway Diagram
Alzheimer's Disease Pathway antibodies	Alzheimer's Disease Pathway Diagram
Alzheimers Disease Pathway antibodies	Alzheimers Disease Pathway Diagram
Amyotrophic Lateral Sclerosis (ALS) Pathway antibodies	Amyotrophic Lateral Sclerosis (ALS) Pathway Diagram
Amyotrophic Lateral Sclerosis (ALS) Pathway antibodies	Amyotrophic Lateral Sclerosis (ALS) Pathway Diagram
Amyotrophic Lateral Sclerosis (ALS) Pathway antibodies	Amyotrophic Lateral Sclerosis (ALS) Pathway Diagram

Diseases associated with Casp-9 elisa kit

Disease Name	Pubmed Publications
Necrosis Antibodies	>1318 publications with Casp-9 and Necrosis
Nervous System Diseases Antibodies	>523 publications with Casp-9 and Nervous System Diseases
Brain Diseases Antibodies	>425 publications with Casp-9 and Brain Diseases
Breast Neoplasms Antibodies	>375 publications with Casp-9 and Breast Neoplasms
Liver Diseases Antibodies	>356 publications with Casp-9 and Liver Diseases
Inflammation Antibodies	>322 publications with Casp-9 and Inflammation
Lung Neoplasms Antibodies	>316 publications with Casp-9 and Lung Neoplasms
Liver Neoplasms Antibodies	>282 publications with Casp-9 and Liver Neoplasms
Carcinoma, Hepatocellular Antibodies	>281 publications with Casp-9 and Carcinoma, Hepatocellular
Neoplasms, Experimental Antibodies	>277 publications with Casp-9 and Neoplasms, Experimental

Organs/Tissues associated with Casp-9 elisa kit

Organ/Tissue Name	Pubmed Publications
Blood Antibodies	>892 publications with Casp-9 and Blood
Brain Antibodies	>606 publications with Casp-9 and Brain
Liver Antibodies	>451 publications with Casp-9 and Liver
Lung Antibodies	>417 publications with Casp-9 and Lung
Muscle Antibodies	>355 publications with Casp-9 and Muscle
Vascular Antibodies	>277 publications with Casp-9 and Vascular
Heart Antibodies	>265 publications with Casp-9 and Heart
Prostate Antibodies	>245 publications with Casp-9 and Prostate
Kidney Antibodies	>220 publications with Casp-9 and Kidney
Bone Antibodies	>189 publications with Casp-9 and Bone