Leucocyte Antigen B27, HLA-B27 (HLA-B27) ELISA Kit for Human

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to HLA-B27. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for HLA-B27 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain HLA-B27, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of HLA-B27 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Small volumes of HLA-B27 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS702823 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the leucocyte antigen B27, HLA-B27, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing HLA-B27. The ELISA analytical biochemical technique of the MBS702823 kit is based on HLA-B27 antibody-HLA-B27 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect HLA-B27 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, HLA-B27. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).