Leptospira IgG (LEPTOSPIRA) ELISA Kit for Human

Principle of the Assay: This assay employs the qualitative enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with antigen. Samples are pipetted into the wells and any antibodies specific for the antigen present will bind to the pre-coated antigen. After removal of unbound material, alkaline phosphatase-conjugated anti-human IgG is added to each well and react with the immune complex. Finally, substrate solution rho-nitrophenylphosphate is added to each well. The enzyme-substrate reaction is terminated by the addition of sodium hydroxide and the color change is measured spectrophotometrically at a wavelength of 405 nm +/- 2 nm. Calculate the concentration of human leptospira IgG in the samples.

Small volumes of Leptospira elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS703382 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Leptospira IgG, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing Leptospira. The ELISA analytical biochemical technique of the MBS703382 kit is based on Leptospira antibody-Leptospira antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect Leptospira antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, Leptospira. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).