Prosaposin (PSAP) ELISA Kit for Human

Name: Human PSAP ELISA Kit; Prosaposin; MyBioSource
Brand: MyBioSource
SKU: MBS703488
Price: 295 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS703488

Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for PSAP has been pre-coated onto a microplate. Standards and samples are pipetted into the wells with a Horseradish Peroxidase (HRP)… More details

$295 / 24-Strip-Wells (LIMIT 1) +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

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Product Overview

Online Datasheet in PDF

Product Name

prosaposin (PSAP), ELISA Kit

Full Product Name

human prosaposin, PSAP ELISA Kit

Product Gene Name

PSAP elisa kit

Product Synonym Names

human prosaposin (PSAP) Elisa kit; FLJ00245; GLBA; MGC110993; SAP1; sphingolipid activator protein-1; prosaposin

Species Reactivity

Human

Assay Type

Sandwich

Samples

Serum, plasma, tissue homogenates

Detection Range

0.625 ng/ml -20 ng/ml.

Sensitivity

The minimum detectable dose of human PSAP is typically less than 0.312 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.

Specificity

This assay has high sensitivity and excellent specificity for detection of human PSAP. No significant cross-reactivity or interference between human PSAP and analogues was observed.

Preparation and Storage

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Inter-assay Precision

Inter-assay Precision (Precision between assays): CV%<20%. Three samples of known concentration were tested in twenty assays to assess.

Intra-assay Precision

Intra-assay Precision (Precision within an assay): CV%<15%. Three samples of known concentration were tested twenty times on one plate to assess.

Molecular Weight

58,113 Da

OMIM

611722

3D Structure

ModBase 3D Structure for P07602

ISO Certification

Manufactured in an ISO 9001:2015 Certified Laboratory.

Other Notes

Small volumes of PSAP elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for PSAP purchase

MBS703488 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the prosaposin (PSAP) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing PSAP. The ELISA analytical biochemical technique of the MBS703488 kit is based on PSAP antibody-PSAP antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect PSAP antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, PSAP. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for PSAP. It may not necessarily be applicable to this product.

NCBI GI #

110224476

NCBI GeneID

5660

NCBI Accession #

NP_001035930.1

UniProt Primary Accession #

P07602

UniProt Secondary Accession #

P07292; P15793; P78538; P78541; P78546; P78547; P78558; Q53Y86; Q6IBQ6; Q92739; Q92740

UniProt Related Accession #

P07602

GenBank Nucleotide #

NM_001042465.1

NCBI Official Full Name

prosaposin isoform b preproprotein

NCBI Official Synonym Full Names

prosaposin

NCBI Official Symbol

PSAP

NCBI Official Synonym Symbols

GLBA; SAP1

UniProt Protein Name

Prosaposin

UniProt Synonym Protein Names

Proactivator polypeptideCleaved into the following 5 chains:Saposin-AAlternative name(s):Protein A

Protein Family

Prosaposin

UniProt Gene Name

PSAP

UniProt Synonym Gene Names

GLBA; SAP1; CSAct; SAP-1; SAP-2

UniProt Entry Name

SAP_HUMAN

NCBI Protein Information

prosaposin; proactivator polypeptide; sphingolipid activator protein-1

NCBI Summary for PSAP

This gene encodes a highly conserved glycoprotein which is a precursor for 4 cleavage products: saposins A, B, C, and D. Each domain of the precursor protein is approximately 80 amino acid residues long with nearly identical placement of cysteine residues and glycosylation sites. Saposins A-D localize primarily to the lysosomal compartment where they facilitate the catabolism of glycosphingolipids with short oligosaccharide groups. The precursor protein exists both as a secretory protein and as an integral membrane protein and has neurotrophic activities. Mutations in this gene have been associated with Gaucher disease, Tay-Sachs disease, and metachromatic leukodystrophy. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]

UniProt Comments for PSAP

Function: Saposin-A and saposin-C stimulate the hydrolysis of glucosylceramide by beta-glucosylceramidase (EC 3.2.1.45) and galactosylceramide by beta-galactosylceramidase (EC 3.2.1.46). Saposin-C apparently acts by combining with the enzyme and acidic lipid to form an activated complex, rather than by solubilizing the substrate.2 PublicationsManual assertion based on experiment in:Ref.24

Research Articles on PSAP

1. Of the 2575 proteins identified, proteins upregulated in gallbladder cancer included several lysosomal proteins such as prosaposin, cathepsin Z and cathepsin H.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with PSAP elisa kit

Reference Product	PubMed Publications
GBA elisa kit	>3 publications with PSAP and GBA
CTSD elisa kit	>2 publications with PSAP and CTSD
CD1B elisa kit	>1 publications with PSAP and CD1B

Pathways associated with PSAP elisa kit

Products by Pathway	Pathway Diagram
Class A/1 (Rhodopsin-like Receptors) Pathway antibodies	Class A/1 (Rhodopsin-like Receptors) Pathway Diagram
GPCR Ligand Binding Pathway antibodies	GPCR Ligand Binding Pathway Diagram
Glycosphingolipid Metabolism Pathway antibodies	Glycosphingolipid Metabolism Pathway Diagram
Hemostasis Pathway antibodies	Hemostasis Pathway Diagram
Lysosome Pathway antibodies	Lysosome Pathway Diagram
Lysosome Pathway antibodies	Lysosome Pathway Diagram
Metabolism Pathway antibodies	Metabolism Pathway Diagram
Metabolism Of Lipids And Lipoproteins Pathway antibodies	Metabolism Of Lipids And Lipoproteins Pathway Diagram
Peptide Ligand-binding Receptors Pathway antibodies	Peptide Ligand-binding Receptors Pathway Diagram
Platelet Activation, Signaling And Aggregation Pathway antibodies	Platelet Activation, Signaling And Aggregation Pathway Diagram

Diseases associated with PSAP elisa kit

Disease Name	Pubmed Publications
Nervous System Diseases Antibodies	>131 publications with PSAP and Nervous System Diseases
Brain Diseases Antibodies	>109 publications with PSAP and Brain Diseases
Adenocarcinoma Antibodies	>46 publications with PSAP and Adenocarcinoma
Metachromatic Leukodystrophy due to Saposin B Deficiency Antibodies	>27 publications with PSAP and Metachromatic Leukodystrophy due to Saposin B Deficiency
Skin Diseases Antibodies	>23 publications with PSAP and Skin Diseases
Liver Diseases Antibodies	>19 publications with PSAP and Liver Diseases
Hyperplasia Antibodies	>13 publications with PSAP and Hyperplasia
Atrophy Antibodies	>13 publications with PSAP and Atrophy
Breast Neoplasms Antibodies	>13 publications with PSAP and Breast Neoplasms
Nerve Degeneration Antibodies	>10 publications with PSAP and Nerve Degeneration

Organs/Tissues associated with PSAP elisa kit

Organ/Tissue Name	Pubmed Publications
Brain Antibodies	>92 publications with PSAP and Brain
Prostate Antibodies	>74 publications with PSAP and Prostate
Blood Antibodies	>57 publications with PSAP and Blood
Liver Antibodies	>47 publications with PSAP and Liver
Nerve Antibodies	>43 publications with PSAP and Nerve
Testis Antibodies	>41 publications with PSAP and Testis
Kidney Antibodies	>37 publications with PSAP and Kidney
Skin Antibodies	>31 publications with PSAP and Skin
Spleen Antibodies	>24 publications with PSAP and Spleen
Muscle Antibodies	>18 publications with PSAP and Muscle