Laminin, Alpha 5 (LAMA5) ELISA Kit for Human

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to LN-5. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for LN-5 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain LN-5, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of LN-5 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Small volumes of LAMA5 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS703569 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the laminin, alpha 5 (LAMA5) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing LAMA5. The ELISA analytical biochemical technique of the MBS703569 kit is based on LAMA5 antibody-LAMA5 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect LAMA5 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, LAMA5. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).