Prostaglandin-Endoperoxide Synthase 2 (Prostaglandin G/H Synthase and Cyclooxygenase) (PTGS2) ELISA Kit for Human

Name: Human PTGS2 ELISA Kit; MyBioSource
Brand: MyBioSource
SKU: MBS703694
Price: 295 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS703694

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for COX-2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any COX-2 present is bound by the… More details

$295 / 24-Strip-Wells (LIMIT 1) +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

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Product Overview

Online Datasheet in PDF

Product Name

prostaglandin-endoperoxide synthase 2 (prostaglandin G/H synthase and cyclooxygenase) (PTGS2), ELISA Kit

Full Product Name

Human cyclooxygenase-2, COX-2 ELISA Kit

Product Gene Name

PTGS2 elisa kit

Product Synonym Names

Human cyclooxygenase-2; COX-2 ELISA Kit; COX-2; COX2; GRIPGHS; PGG/HS; PGHS-2; PHS-2; hCox-2; cyclooxygenase 2b; prostaglandin G/H synthase and cyclooxygenase; prostaglandin-endoperoxide synthase 2; prostaglandin-endoperoxide synthase 2 (prostaglandin G/H synthase and cyclooxygenase)

Species Reactivity

Human

Assay Type

Sandwich

Samples

Serum, plasma, tissue homogenates, Cell lysates

Detection Range

1.25 ng/ml-80 ng/ml

Sensitivity

0.31 ng/ml

Specificity

This assay has high sensitivity and excellent specificity for detection of Human PTGS2. No significant cross-reactivity or interference between Human PTGS2 and analogues was observed.

Preparation and Storage

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Intra-assay Precision

Intra-assay Precision (Precision within an assay): CV% is less than 8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV% is less than 10%
Three samples of known concentration were tested in twenty assays to assess.

Molecular Weight

68,996 Da

OMIM

600262

3D Structure

ModBase 3D Structure for P35354

Detection Wavelength

450 nm

Sample Volume

50-100ul

Protein Biological Process 1

Biosynthesis/Metabolism

Protein Biological Process 2

Lipogenesis and lipometabolism

Protein Biological Process 3

Fatty acid biosynthesis

ISO Certification

Manufactured in an ISO 9001:2015 Certified Laboratory.

Other Notes

Small volumes of PTGS2 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for PTGS2 purchase

MBS703694 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the prostaglandin-endoperoxide synthase 2 (prostaglandin G/H synthase and cyclooxygenase) (PTGS2) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing PTGS2. The ELISA analytical biochemical technique of the MBS703694 kit is based on PTGS2 antibody-PTGS2 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect PTGS2 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, PTGS2. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for PTGS2. It may not necessarily be applicable to this product.

NCBI GI #

4506265

NCBI GeneID

5743

NCBI Accession #

NP_000954.1

UniProt Primary Accession #

P35354

UniProt Secondary Accession #

Q16876; A8K802

UniProt Related Accession #

P35354

GenBank Nucleotide #

NM_000963.3

NCBI Official Full Name

prostaglandin G/H synthase 2

NCBI Official Synonym Full Names

prostaglandin-endoperoxide synthase 2 (prostaglandin G/H synthase and cyclooxygenase)

NCBI Official Symbol

PTGS2

NCBI Official Synonym Symbols

COX2; COX-2; PHS-2; PGG/HS; PGHS-2; hCox-2; GRIPGHS

UniProt Protein Name

Prostaglandin G/H synthase 2

UniProt Synonym Protein Names

Cyclooxygenase-2; COX-2; PHS II; Prostaglandin H2 synthase 2; PGH synthase 2; PGHS-2; Prostaglandin-endoperoxide synthase 2

Protein Family

Prostaglandin G/H synthase

UniProt Gene Name

PTGS2

UniProt Synonym Gene Names

COX2; COX-2; PGH synthase 2; PGHS-2

UniProt Entry Name

PGH2_HUMAN

NCBI Protein Information

prostaglandin G/H synthase 2; PHS II; PGH synthase 2; cyclooxygenase 2b; prostaglandin H2 synthase 2

NCBI Summary for PTGS2

Prostaglandin-endoperoxide synthase (PTGS), also known as cyclooxygenase, is the key enzyme in prostaglandin biosynthesis, and acts both as a dioxygenase and as a peroxidase. There are two isozymes of PTGS: a constitutive PTGS1 and an inducible PTGS2, which differ in their regulation of expression and tissue distribution. This gene encodes the inducible isozyme. It is regulated by specific stimulatory events, suggesting that it is responsible for the prostanoid biosynthesis involved in inflammation and mitogenesis. [provided by RefSeq, Feb 2009]

UniProt Comments for PTGS2

Function: Converts arachidonate to prostaglandin H2 (PGH2), a committed step in prostanoid synthesis. Constitutively expressed in some tissues in physiological conditions, such as the endothelium, kidney and brain, and in pathological conditions, such as in cancer. PTGS2 is responsible for production of inflammatory prostaglandins. Up-regulation of PTGS2 is also associated with increased cell adhesion, phenotypic changes, resistance to apoptosis and tumor angiogenesis. In cancer cells, PTGS2 is a key step in the production of prostaglandin E2 (PGE2), which plays important roles in modulating motility, proliferation and resistance to apoptosis.1 PublicationManual assertion based on experiment in:Ref.13

Catalytic activity: Arachidonate + AH2 + 2 O2 = prostaglandin H2 + A + H2O.1 PublicationManual assertion based on experiment in:Ref.13

Cofactor: Binds 1 heme B (iron-protoporphyrin IX) group per subunit

Research Articles on PTGS2

1. COX-2 and Bcl-2 overexpression in the CG group suggests that CG, particularly the intestinal type, may be a premalignant lesion that converts into a tumor in the presence of carcinogens.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with PTGS2 elisa kit

Reference Product	PubMed Publications
JUN elisa kit	>2735 publications with PTGS2 and JUN
PLA2G4A elisa kit	>295 publications with PTGS2 and PLA2G4A
ALOX5 elisa kit	>272 publications with PTGS2 and ALOX5
MAPK1 elisa kit	>254 publications with PTGS2 and MAPK1
FOS elisa kit	>231 publications with PTGS2 and FOS
PTGES elisa kit	>46 publications with PTGS2 and PTGES
CYP2E1 elisa kit	>34 publications with PTGS2 and CYP2E1
MAPK14 elisa kit	>8 publications with PTGS2 and MAPK14
PTGDS elisa kit	>6 publications with PTGS2 and PTGDS
PLA2G1B elisa kit	>3 publications with PTGS2 and PLA2G1B

Pathways associated with PTGS2 elisa kit

Products by Pathway	Pathway Diagram
Arachidonic Acid Metabolism Pathway antibodies	Arachidonic Acid Metabolism Pathway Diagram
Arachidonic Acid Metabolism Pathway antibodies	Arachidonic Acid Metabolism Pathway Diagram
Arachidonic Acid Metabolism Pathway antibodies	Arachidonic Acid Metabolism Pathway Diagram
C-MYB Transcription Factor Network Pathway antibodies	C-MYB Transcription Factor Network Pathway Diagram
C20 Prostanoid Biosynthesis Pathway antibodies	C20 Prostanoid Biosynthesis Pathway Diagram
Calcineurin-regulated NFAT-dependent Transcription In Lymphocytes Pathway antibodies	Calcineurin-regulated NFAT-dependent Transcription In Lymphocytes Pathway Diagram
Calcium Signaling In The CD4+ TCR Pathway antibodies	Calcium Signaling In The CD4+ TCR Pathway Diagram
Chemical Carcinogenesis Pathway antibodies	Chemical Carcinogenesis Pathway Diagram
Chemical Carcinogenesis Pathway antibodies	Chemical Carcinogenesis Pathway Diagram
Defective AMN Causes Hereditary Megaloblastic Anemia 1 Pathway antibodies	Defective AMN Causes Hereditary Megaloblastic Anemia 1 Pathway Diagram

Diseases associated with PTGS2 elisa kit

Disease Name	Pubmed Publications
Inflammation Antibodies	>6189 publications with PTGS2 and Inflammation
Carcinoma Antibodies	>3024 publications with PTGS2 and Carcinoma
Nervous System Diseases Antibodies	>2198 publications with PTGS2 and Nervous System Diseases
Colorectal Neoplasms Antibodies	>1681 publications with PTGS2 and Colorectal Neoplasms
Adenocarcinoma Antibodies	>1434 publications with PTGS2 and Adenocarcinoma
Skin Diseases Antibodies	>1140 publications with PTGS2 and Skin Diseases
Lung Diseases Antibodies	>1005 publications with PTGS2 and Lung Diseases
Colonic Neoplasms Antibodies	>949 publications with PTGS2 and Colonic Neoplasms
Edema Antibodies	>886 publications with PTGS2 and Edema
Breast Neoplasms Antibodies	>727 publications with PTGS2 and Breast Neoplasms

Organs/Tissues associated with PTGS2 elisa kit

Organ/Tissue Name	Pubmed Publications
Blood Antibodies	>3901 publications with PTGS2 and Blood
Vascular Antibodies	>2642 publications with PTGS2 and Vascular
Brain Antibodies	>1599 publications with PTGS2 and Brain
Lung Antibodies	>1285 publications with PTGS2 and Lung
Muscle Antibodies	>1168 publications with PTGS2 and Muscle
Stomach Antibodies	>1105 publications with PTGS2 and Stomach
Kidney Antibodies	>1066 publications with PTGS2 and Kidney
Liver Antibodies	>1021 publications with PTGS2 and Liver
Bone Antibodies	>921 publications with PTGS2 and Bone
Intestine Antibodies	>873 publications with PTGS2 and Intestine