Orexin B, Ox-B (OX-B) ELISA Kit for Human

Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for OX-B has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any OX-B present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for OX-B is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of OX-B bound in the initial step. The color development is stopped and the intensity of the color is measured.

Small volumes of OX-B elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS703745 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Orexin B, OX-B, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing OX-B. The ELISA analytical biochemical technique of the MBS703745 kit is based on OX-B antibody-OX-B antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect OX-B antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, OX-B. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).