Granzyme B (Granzyme 2, Cytotoxic T-Lymphocyte-Associated Serine Esterase 1) (GZMB) ELISA Kit for Human

Name: Human GZMB ELISA Kit; MyBioSource
Brand: MyBioSource
SKU: MBS703753
Price: 295 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS703753

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for GZMB has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any GZMB present is bound by the… More details

$295 / 24-Strip-Wells (LIMIT 1) +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

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Product Overview

Online Datasheet in PDF

Product Name

granzyme B (granzyme 2, cytotoxic T-lymphocyte-associated serine esterase 1) (GZMB), ELISA Kit

Full Product Name

Human granzymes B, Gzms-B ELISA Kit

Product Gene Name

GZMB elisa kit

Product Synonym Names

Human granzymes B; Gzms-B ELISA Kit; CCPI; CGL-1; CGL1; CSP-B; CSPB; CTLA1; CTSGL1; HLP; SECT; T-cell serine protease 1-3E; cathepsin G-like 1; cytotoxic serine protease B; fragmentin 2; granzyme B; granzyme B (granzyme 2; cytotoxic T-lymphocyte-associated serine esterase 1)

Species Reactivity

Human

Assay Type

Quantitative Sandwich

Samples

Serum, plasma, tissue homogenates

Detection Range

1.56 pg/ml-100 pg/ml

Sensitivity

< 0.39 pg/ml

Specificity

This assay has high sensitivity and excellent specificity for detection of human GZMB. No significant cross-reactivity or interference between human GZMB and analogues was observed.

Preparation and Storage

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Inter-assay Precision

Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

Intra-assay Precision

Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.

Molecular Weight

27,716 Da

OMIM

123910

3D Structure

ModBase 3D Structure for P10144

ISO Certification

Manufactured in an ISO 9001:2015 Certified Laboratory.

Other Notes

Small volumes of GZMB elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for GZMB purchase

MBS703753 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the granzyme B (granzyme 2, cytotoxic T-lymphocyte-associated serine esterase 1) (GZMB) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing GZMB. The ELISA analytical biochemical technique of the MBS703753 kit is based on GZMB antibody-GZMB antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect GZMB antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, GZMB. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for GZMB. It may not necessarily be applicable to this product.

NCBI GI #

221625528

NCBI GeneID

3002

NCBI Accession #

NP_004122.2

UniProt Primary Accession #

P10144

UniProt Secondary Accession #

Q8N1D2; Q9UCC1

UniProt Related Accession #

P10144

GenBank Nucleotide #

NM_004131.4

NCBI Official Full Name

granzyme B

NCBI Official Synonym Full Names

granzyme B (granzyme 2, cytotoxic T-lymphocyte-associated serine esterase 1)

NCBI Official Symbol

GZMB

NCBI Official Synonym Symbols

HLP; CCPI; CGL1; CSPB; SECT; CGL-1; CSP-B; CTLA1; CTSGL1

UniProt Protein Name

Granzyme B

UniProt Synonym Protein Names

C11; CTLA-1; Cathepsin G-like 1; CTSGL1; Cytotoxic T-lymphocyte proteinase 2; Lymphocyte protease; Fragmentin-2; Granzyme-2; Human lymphocyte protein; HLP; SECT; T-cell serine protease 1-3E

Protein Family

Granzyme

UniProt Gene Name

GZMB

UniProt Synonym Gene Names

CGL1; CSPB; CTLA1; GRB; CTSGL1; Lymphocyte protease; HLP

UniProt Entry Name

GRAB_HUMAN

NCBI Protein Information

granzyme B; C11; CTLA-1; fragmentin 2; fragmentin-2; cathepsin G-like 1; human lymphocyte protein; T-cell serine protease 1-3E; cytotoxic serine protease B; cytotoxic T-lymphocyte proteinase 2

NCBI Summary for GZMB

Cytolytic T lymphocytes (CTL) and natural killer (NK) cells share the remarkable ability to recognize, bind, and lyse specific target cells. They are thought to protect their host by lysing cells bearing on their surface 'nonself' antigens, usually peptides or proteins resulting from infection by intracellular pathogens. The protein encoded by this gene is crucial for the rapid induction of target cell apoptosis by CTL in cell-mediated immune response. [provided by RefSeq, Jul 2008]

UniProt Comments for GZMB

Function: This enzyme is necessary for target cell lysis in cell-mediated immune responses. It cleaves after Asp. Seems to be linked to an activation cascade of caspases (aspartate-specific cysteine proteases) responsible for apoptosis execution. Cleaves caspase-3, -7, -9 and 10 to give rise to active enzymes mediating apoptosis.

Catalytic activity: Preferential cleavage: -Asp-|-Xaa- >> -Asn-|-Xaa- > -Met-|-Xaa-, -Ser-|-Xaa-.1 PublicationManual assertion based on experiment in:Ref.12

Enzyme regulation: Inactivated by the serine protease inhibitor diisopropylfluorophosphate.1 PublicationManual assertion based on experiment in:Ref.12

Research Articles on GZMB

1. These data indicate that IBD-related inflammation is marked by mucosal accumulation of cytotoxic, GrB-expressing CD19(+) and IgA(+) cells, suggesting a role for these cells in IBD-associated epithelial damage.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with GZMB elisa kit

Reference Product	PubMed Publications
PRF1 elisa kit	>1244 publications with GZMB and PRF1
CASP3 elisa kit	>141 publications with GZMB and CASP3
BID elisa kit	>57 publications with GZMB and BID
SERPINB9 elisa kit	>57 publications with GZMB and SERPINB9
IL2RA elisa kit	>48 publications with GZMB and IL2RA
CASP7 elisa kit	>19 publications with GZMB and CASP7
IL2RB elisa kit	>14 publications with GZMB and IL2RB
IGF2R elisa kit	>13 publications with GZMB and IGF2R
IL2 elisa kit	>6 publications with GZMB and IL2

Pathways associated with GZMB elisa kit

Products by Pathway	Pathway Diagram
Activation, Myristolyation Of BID And Translocation To Mitochondria Pathway antibodies	Activation, Myristolyation Of BID And Translocation To Mitochondria Pathway Diagram
Allograft Rejection Pathway antibodies	Allograft Rejection Pathway Diagram
Allograft Rejection Pathway antibodies	Allograft Rejection Pathway Diagram
Allograft Rejection Pathway antibodies	Allograft Rejection Pathway Diagram
Apoptosis Pathway antibodies	Apoptosis Pathway Diagram
Apoptosis Pathway antibodies	Apoptosis Pathway Diagram
Autoimmune Thyroid Disease Pathway antibodies	Autoimmune Thyroid Disease Pathway Diagram
Autoimmune Thyroid Disease Pathway antibodies	Autoimmune Thyroid Disease Pathway Diagram
Caspase Cascade In Apoptosis Pathway antibodies	Caspase Cascade In Apoptosis Pathway Diagram
Downstream Signaling In Naive CD8+ T Cells Pathway antibodies	Downstream Signaling In Naive CD8+ T Cells Pathway Diagram

Diseases associated with GZMB elisa kit

Disease Name	Pubmed Publications
Necrosis Antibodies	>303 publications with GZMB and Necrosis
Inflammation Antibodies	>192 publications with GZMB and Inflammation
Disease Models, Animal Antibodies	>112 publications with GZMB and Disease Models, Animal
Nervous System Diseases Antibodies	>95 publications with GZMB and Nervous System Diseases
Neoplasms, Experimental Antibodies	>95 publications with GZMB and Neoplasms, Experimental
Liver Diseases Antibodies	>74 publications with GZMB and Liver Diseases
Hepatitis Antibodies	>54 publications with GZMB and Hepatitis
Brain Diseases Antibodies	>35 publications with GZMB and Brain Diseases
Breast Neoplasms Antibodies	>33 publications with GZMB and Breast Neoplasms
Kidney Diseases Antibodies	>27 publications with GZMB and Kidney Diseases

Organs/Tissues associated with GZMB elisa kit

Organ/Tissue Name	Pubmed Publications
Blood Antibodies	>806 publications with GZMB and Blood
Kidney Antibodies	>80 publications with GZMB and Kidney
Lung Antibodies	>62 publications with GZMB and Lung
Liver Antibodies	>61 publications with GZMB and Liver
Spleen Antibodies	>56 publications with GZMB and Spleen
Bone Marrow Antibodies	>44 publications with GZMB and Bone Marrow
Heart Antibodies	>32 publications with GZMB and Heart
Intestine Antibodies	>28 publications with GZMB and Intestine
Pancreas Antibodies	>13 publications with GZMB and Pancreas
Stomach Antibodies	>10 publications with GZMB and Stomach