Complement Fragment 3a, C3a (C3A) ELISA Kit for Mouse

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for C3a has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any C3a present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for C3a is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of C3a bound in the initial step. The color development is stopped and the intensity of the color is measured.

Small volumes of C3a elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS703819 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Complement fragment 3a, C3a, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing C3a. The ELISA analytical biochemical technique of the MBS703819 kit is based on C3a antibody-C3a antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect C3a antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, C3a. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%). Complement fragment 3a C3a is part of the complex complement factor system, which regulates many inflammatory pathways. We have amassed considerable information on complement activation via GPCR mediated signaling but much research is still needed. To aid your assay of serum or plasma of complement C3a proteins, MyBiosource has reagents specifically designed for reactivity to C3a cell samples. We offer mouse and human C3a ELISA kits for your valuable sample preps. Don't miss any protein, use our sandwich format ELISA kit for best results. Each antibody is quality tested and secondary antibodies carefully selected to maximize detection of all C3a antibody bindings, as described in package insert. For WB comparisons, use our anti complement C3a antibody (monoclonal) against C3a human, reactivity human not mouse. View our online catalog for additional complement C3a ELISA kits to different species to expand your models and expand to factors related to complement component 3a.