Perforin 1 (Pore Forming Protein) (PRF1) ELISA Kit for Human

Name: Human PRF1 ELISA Kit; Perforin 1 (Pore Forming Protein); MyBioSource
Brand: MyBioSource
SKU: MBS703979
Price: 295 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS703979

MBS703979 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the perforin 1 (pore forming protein) (PRF1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit… More details

$295 / 24-Strip-Wells (LIMIT 1) +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Request Current Manual

Product Overview

Online Datasheet in PDF

Product Name

perforin 1 (pore forming protein) (PRF1), ELISA Kit

Full Product Name

Human Perforin-1, PRF1/PFP ELISA Kit

Product Gene Name

PRF1 elisa kit

Product Synonym Names

Human Perforin-1 (PRF1/PFP) ELISA kit; RP11-710A11.3; FLH2; HPLH2; MGC65093; P1; PFN1; PFP; OTTHUMP00000019759; cytolysin; lymphocyte pore forming protein; perforin 1; perforin 1 (pore forming protein)

Species Reactivity

Human

Samples

Serum, plasma, tissue homogenates

Detection Range

0.312 ng/ml-20 ng/ml

Sensitivity

0.078 ng/ml

Specificity

This assay has high sensitivity and excellent specificity for detection of Human PRF1. No significant cross-reactivity or interference between Human PRF1 and analogues was observed.

Preparation and Storage

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Intra-assay Precision

Intra-assay Precision (Precision within an assay): CV% is less than 8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV% is less than 10%
Three samples of known concentration were tested in twenty assays to assess.

Molecular Weight

61,377 Da

OMIM

gene 603553

3D Structure

ModBase 3D Structure for P14222

Detection Wavelength

450 nm

Sample Volume

50-100ul

Protein Biological Process 1

Immunity

Protein Biological Process 3

Cytolysis

ISO Certification

Manufactured in an ISO 9001:2015 Certified Laboratory.

Other Notes

Small volumes of PRF1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for PRF1. It may not necessarily be applicable to this product.

NCBI GI #

133908621

NCBI GeneID

5551

NCBI Accession #

NP_001076585.1

UniProt Primary Accession #

P14222

UniProt Secondary Accession #

Q59F57; Q86WX7; B2R6X4

UniProt Related Accession #

P14222

GenBank Nucleotide #

NM_001083116.1

NCBI Official Full Name

perforin-1

NCBI Official Synonym Full Names

perforin 1 (pore forming protein)

NCBI Official Symbol

PRF1

NCBI Official Synonym Symbols

P1; PFP; FLH2; PFN1; HPLH2

UniProt Protein Name

Perforin-1

UniProt Synonym Protein Names

Cytolysin; Lymphocyte pore-forming protein; PFP

Protein Family

Peptide chain release factor

UniProt Gene Name

PRF1

UniProt Synonym Gene Names

PFP; P1; PFP

UniProt Entry Name

PERF_HUMAN

NCBI Protein Information

perforin-1; cytolysin; lymphocyte pore forming protein; lymphocyte pore-forming protein

NCBI Summary for PRF1

The protein encoded by this gene has structural and functional similarities to complement component 9 (C9). Like C9, this protein creates transmembrane tubules and is capable of lysing non-specifically a variety of target cells. This protein is one of the main cytolytic proteins of cytolytic granules, and it is known to be a key effector molecule for T-cell- and natural killer-cell-mediated cytolysis. Defects in this gene cause familial hemophagocytic lymphohistiocytosis type 2 (HPLH2), a rare and lethal autosomal recessive disorder of early childhood. Alternative splicing results in multiple transcript variants encoding the same protein. [provided by RefSeq, Jul 2008]

UniProt Comments for PRF1

Function: Plays a key role in secretory granule-dependent cell death, and in defense against virus-infected or neoplastic cells. Plays an important role in killing other cells that are recognized as non-self by the immune system, e.g. in transplant rejection or some forms of autoimmune disease. Can insert into the membrane of target cells in its calcium-bound form, oligomerize and form large pores. Promotes cytolysis and apoptosis of target cells by facilitating the uptake of cytotoxic granzymes.6 PublicationsManual assertion based on experiment in:Ref.10

Research Articles on PRF1

1. Exome sequencing identified a biallelic perforin1 mutation (PRF1; p.R225W) previously associated with familial hemophagocytic lymphohistiocytosis.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with PRF1 elisa kit

Reference Product	PubMed Publications
GZMB elisa kit	>1244 publications with PRF1 and GZMB
CD80 elisa kit	>62 publications with PRF1 and CD80
IL2RA elisa kit	>47 publications with PRF1 and IL2RA
CD86 elisa kit	>41 publications with PRF1 and CD86
EOMES elisa kit	>25 publications with PRF1 and EOMES
IL2 elisa kit	>16 publications with PRF1 and IL2
IL2RB elisa kit	>12 publications with PRF1 and IL2RB
CD3E elisa kit	>1 publications with PRF1 and CD3E
CD247 elisa kit	>1 publications with PRF1 and CD247

Pathways associated with PRF1 elisa kit

Products by Pathway	Pathway Diagram
Allograft Rejection Pathway antibodies	Allograft Rejection Pathway Diagram
Allograft Rejection Pathway antibodies	Allograft Rejection Pathway Diagram
Allograft Rejection Pathway antibodies	Allograft Rejection Pathway Diagram
Apoptosis Pathway antibodies	Apoptosis Pathway Diagram
Autoimmune Thyroid Disease Pathway antibodies	Autoimmune Thyroid Disease Pathway Diagram
Autoimmune Thyroid Disease Pathway antibodies	Autoimmune Thyroid Disease Pathway Diagram
Caspase Cascade In Apoptosis Pathway antibodies	Caspase Cascade In Apoptosis Pathway Diagram
Downstream Signaling In Naive CD8+ T Cells Pathway antibodies	Downstream Signaling In Naive CD8+ T Cells Pathway Diagram
Graft-versus-host Disease Pathway antibodies	Graft-versus-host Disease Pathway Diagram
Graft-versus-host Disease Pathway antibodies	Graft-versus-host Disease Pathway Diagram

Diseases associated with PRF1 elisa kit

Disease Name	Pubmed Publications
Necrosis Antibodies	>687 publications with PRF1 and Necrosis
Neoplasms, Experimental Antibodies	>300 publications with PRF1 and Neoplasms, Experimental
Inflammation Antibodies	>298 publications with PRF1 and Inflammation
Carcinoma Antibodies	>208 publications with PRF1 and Carcinoma
Liver Diseases Antibodies	>184 publications with PRF1 and Liver Diseases
Lymphoma, Non-Hodgkin Antibodies	>161 publications with PRF1 and Lymphoma, Non-Hodgkin
Cardiovascular Diseases Antibodies	>144 publications with PRF1 and Cardiovascular Diseases
Lung Neoplasms Antibodies	>81 publications with PRF1 and Lung Neoplasms
Kidney Diseases Antibodies	>67 publications with PRF1 and Kidney Diseases
Heart Diseases Antibodies	>66 publications with PRF1 and Heart Diseases

Organs/Tissues associated with PRF1 elisa kit

Organ/Tissue Name	Pubmed Publications
Blood Antibodies	>82 publications with PRF1 and Blood
Bone Marrow Antibodies	>14 publications with PRF1 and Bone Marrow
Liver Antibodies	>7 publications with PRF1 and Liver
Muscle Antibodies	>3 publications with PRF1 and Muscle
Brain Antibodies	>2 publications with PRF1 and Brain
Thymus Antibodies	>2 publications with PRF1 and Thymus
Lung Antibodies	>2 publications with PRF1 and Lung
Uterus Antibodies	>1 publications with PRF1 and Uterus
Intestine Antibodies	>1 publications with PRF1 and Intestine
Prostate Antibodies	>1 publications with PRF1 and Prostate