CD46 Molecule, Complement Regulatory (CD46) ELISA Kit for Human

Name: Human CD46 ELISA Kit; CD46 Molecule, Complement Regulatory; MyBioSource
Brand: MyBioSource
SKU: MBS704177
Price: 295 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS704177

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for MCP has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any MCP present is bound by the… More details

$295 / 24-Strip-Wells (LIMIT 1) +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

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Product Overview

Online Datasheet in PDF

Product Name

CD46 molecule, complement regulatory protein, ELISA Kit

Full Product Name

Human membrane cofactor protein, MCP ELISA Kit

Product Gene Name

CD46 elisa kit

Product Synonym Names

Human membrane cofactor protein; MCP ELISA Kit; AHUS2; MCP; MGC26544; MIC10; TLX; TRA2.10; CD46 antigen; complement regulatory protein; antigen identified by monoclonal antibody TRA-2-10; complement membrane cofactor protein; measles virus receptor; ; CD46 molecule; complement regulatory protein

Species Reactivity

Human

Assay Type

Quantitative Sandwich

Samples

Serum, plasma, tissue homogenates

Detection Range

3.12 ng/ml-200 ng/ml

Sensitivity

< 0.78 ng/ml

Specificity

This assay has high sensitivity and excellent specificity for detection of human MCP. No significant cross-reactivity or interference between human MCP and analogues was observed.

Preparation and Storage

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Inter-assay Precision

Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

Intra-assay Precision

Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.

Molecular Weight

43,747 Da

OMIM

gene+612922

3D Structure

ModBase 3D Structure for P15529

ISO Certification

Manufactured in an ISO 9001:2015 Certified Laboratory.

Other Notes

Small volumes of CD46 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for CD46 purchase

MBS704177 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the CD46 molecule, complement regulatory protein, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing CD46. The ELISA analytical biochemical technique of the MBS704177 kit is based on CD46 antibody-CD46 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect CD46 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, CD46. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for CD46. It may not necessarily be applicable to this product.

NCBI GI #

27502402

NCBI GeneID

4179

NCBI Accession #

NP_002380.3

UniProt Primary Accession #

P15529

UniProt Secondary Accession #

Q15429; Q53GV9; Q5HY94; Q5VWS6; Q5VWS7; Q5VWS8; Q5VWS9; Q5VWT0; A0T1T0; A0T1T1; A0T1T2

UniProt Related Accession #

P15529

GenBank Nucleotide #

NM_002389.4

NCBI Official Full Name

membrane cofactor protein isoform 1

NCBI Official Synonym Full Names

CD46 molecule, complement regulatory protein

NCBI Official Symbol

CD46

NCBI Official Synonym Symbols

MCP; TLX; AHUS2; MIC10; TRA2.10

UniProt Protein Name

Membrane cofactor protein

UniProt Synonym Protein Names

TLX; Trophoblast leukocyte common antigen; CD_antigen: CD46

Protein Family

Membrane cofactor protein

UniProt Gene Name

CD46

UniProt Synonym Gene Names

MCP; MIC10

UniProt Entry Name

MCP_HUMAN

NCBI Protein Information

membrane cofactor protein; measles virus receptor; complement membrane cofactor protein; trophoblast leucocyte common antigen; trophoblast leukocyte common antigen; CD46 antigen, complement regulatory protein; trophoblast-lymphocyte cross-reactive antigen; antigen identified by monoclonal antibody TRA-2-10; membrane cofactor protein (CD46, trophoblast-lymphocyte cross-reactive antigen)

NCBI Summary for CD46

The protein encoded by this gene is a type I membrane protein and is a regulatory part of the complement system. The encoded protein has cofactor activity for inactivation of complement components C3b and C4b by serum factor I, which protects the host cell from damage by complement. In addition, the encoded protein can act as a receptor for the Edmonston strain of measles virus, human herpesvirus-6, and type IV pili of pathogenic Neisseria. Finally, the protein encoded by this gene may be involved in the fusion of the spermatozoa with the oocyte during fertilization. Mutations at this locus have been associated with susceptibility to hemolytic uremic syndrome. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq, Jun 2010]

UniProt Comments for CD46

Function: Acts as a cofactor for complement factor I, a serine protease which protects autologous cells against complement-mediated injury by cleaving C3b and C4b deposited on host tissue. May be involved in the fusion of the spermatozoa with the oocyte during fertilization. Also acts as a costimulatory factor for T-cells which induces the differentiation of CD4+ into T-regulatory 1 cells. T-regulatory 1 cells suppress immune responses by secreting interleukin-10, and therefore are thought to prevent autoimmunity. A number of viral and bacterial pathogens seem to exploit this property and directly induce an immunosuppressive phenotype in T-cells by binding to CD46.2 PublicationsManual assertion based on experiment in:Ref.29

Research Articles on CD46

1. Data show that calcitriol affects the CD46 pathway, and that it promotes anti-inflammatory responses mediated by CD46.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with CD46 elisa kit

Products by Pathway	Pathway Diagram
Complement And Coagulation Cascades Pathway antibodies	Complement And Coagulation Cascades Pathway Diagram
Complement And Coagulation Cascades Pathway antibodies	Complement And Coagulation Cascades Pathway Diagram
Complement And Coagulation Cascades Pathway antibodies	Complement And Coagulation Cascades Pathway Diagram
Complement Cascade Pathway antibodies	Complement Cascade Pathway Diagram
Immune System Pathway antibodies	Immune System Pathway Diagram
Innate Immune System Pathway antibodies	Innate Immune System Pathway Diagram
Measles Pathway antibodies	Measles Pathway Diagram
Measles Pathway antibodies	Measles Pathway Diagram
Regulation Of Complement Cascade Pathway antibodies	Regulation Of Complement Cascade Pathway Diagram

Diseases associated with CD46 elisa kit

Disease Name	Pubmed Publications
Measles Antibodies	>367 publications with CD46 and Measles
Inflammation Antibodies	>96 publications with CD46 and Inflammation
Nervous System Diseases Antibodies	>84 publications with CD46 and Nervous System Diseases
Necrosis Antibodies	>62 publications with CD46 and Necrosis
Atypical hemolytic uremic syndrome Antibodies	>53 publications with CD46 and Atypical hemolytic uremic syndrome
Brain Diseases Antibodies	>34 publications with CD46 and Brain Diseases
Lung Diseases Antibodies	>30 publications with CD46 and Lung Diseases
Liver Diseases Antibodies	>29 publications with CD46 and Liver Diseases
Breast Neoplasms Antibodies	>26 publications with CD46 and Breast Neoplasms
Nephritis Antibodies	>23 publications with CD46 and Nephritis

Organs/Tissues associated with CD46 elisa kit

Organ/Tissue Name	Pubmed Publications
Blood Antibodies	>500 publications with CD46 and Blood
Kidney Antibodies	>109 publications with CD46 and Kidney
Vascular Antibodies	>94 publications with CD46 and Vascular
Brain Antibodies	>92 publications with CD46 and Brain
Liver Antibodies	>74 publications with CD46 and Liver
Lung Antibodies	>72 publications with CD46 and Lung
Heart Antibodies	>56 publications with CD46 and Heart
Bone Antibodies	>47 publications with CD46 and Bone
Skin Antibodies	>41 publications with CD46 and Skin
Muscle Antibodies	>37 publications with CD46 and Muscle