Integrin, Beta 2 (Complement Component 3 Receptor 3 and 4 Subunit) (ITGB2) ELISA Kit for Human

Name: Human ITGB2 ELISA Kit; MyBioSource
Brand: MyBioSource
SKU: MBS704374
Price: 295 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS704374

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for ITG beta2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any ITG beta2 present is bound by… More details

$295 / 24-Strip-Wells (LIMIT 1) +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

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Product Overview

Online Datasheet in PDF

Product Name

integrin, beta 2 (complement component 3 receptor 3 and 4 subunit) (ITGB2), ELISA Kit

Full Product Name

Human integrin beta2, ITG beta2/CD11+CD18 ELISA Kit

Product Gene Name

ITGB2 elisa kit

Product Synonym Names

Human integrin beta2; ITG beta2/CD11+CD18 ELISA Kit; CD18; LAD; LCAMB; LFA-1; MAC-1; MF17; MFI7; OTTHUMP00000115279; OTTHUMP00000115281; OTTHUMP00000115282; cell surface adhesion glycoprotein LFA-1/CR3/P150; 959 beta subunit precursor) ; complement receptor; integrin; beta 2 (complement component 3 receptor 3 and 4 subunit)

Species Reactivity

Human

Assay Type

Sandwich (Quantitative)

Samples

Serum, plasma, tissue homogenates.

Detection Range

15.6 pg/ml-1000 pg/ml.

Sensitivity

3.9 pg/ml.

Specificity

This assay has high sensitivity and excellent specificity for detection of human ITG beta2. No significant cross-reactivity or interference between human ITG beta2 and analogues was observed.

Preparation and Storage

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Inter-assay Precision

Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

Intra-assay Precision

Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.

Molecular Weight

84,782 Da

OMIM

600065

3D Structure

ModBase 3D Structure for P05107

ISO Certification

Manufactured in an ISO 9001:2015 Certified Laboratory.

Other Notes

Small volumes of ITGB2 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for ITGB2 purchase

MBS704374 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the integrin, beta 2 (complement component 3 receptor 3 and 4 subunit) (ITGB2) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing ITGB2. The ELISA analytical biochemical technique of the MBS704374 kit is based on ITGB2 antibody-ITGB2 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect ITGB2 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, ITGB2. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for ITGB2. It may not necessarily be applicable to this product.

NCBI GI #

89191865

NCBI GeneID

3689

NCBI Accession #

NP_000202.2

UniProt Primary Accession #

P05107

UniProt Secondary Accession #

Q16418; Q53HS5; Q9UD72; B3KTS8; D3DSM1

UniProt Related Accession #

P05107

GenBank Nucleotide #

NM_000211.3

NCBI Official Full Name

integrin beta-2

NCBI Official Synonym Full Names

integrin, beta 2 (complement component 3 receptor 3 and 4 subunit)

NCBI Official Symbol

ITGB2

NCBI Official Synonym Symbols

LAD; CD18; MF17; MFI7; LCAMB; LFA-1; MAC-1

UniProt Protein Name

Integrin beta-2

UniProt Synonym Protein Names

Cell surface adhesion glycoproteins LFA-1/CR3/p150,95 subunit beta; Complement receptor C3 subunit beta; CD_antigen: CD18

Protein Family

Integrin

UniProt Gene Name

ITGB2

UniProt Synonym Gene Names

CD18; MFI7

UniProt Entry Name

ITB2_HUMAN

NCBI Protein Information

integrin beta-2; integrin beta chain, beta 2; complement receptor C3 beta-subunit; complement receptor C3 subunit beta; leukocyte cell adhesion molecule CD18; leukocyte-associated antigens CD18/11A, CD18/11B, CD18/11C; cell surface adhesion glycoproteins LFA-1/CR3/p150,95 subunit beta; cell surface adhesion glycoprotein LFA-1/CR3/P150,959 beta subunit precursor)

NCBI Summary for ITGB2

The product of this gene belongs to the integrin beta chain family of proteins. Integrins are integral cell-surface proteins composed of an alpha chain and a beta chain. This gene encodes the integrin beta chain beta 2. A given chain may combine with multiple partners resulting in different integrins. For example, beta 2 combines with the alpha L chain to form the integrin LFA-1, and combines with the alpha M chain to form the integrin Mac-1. Integrins are known to participate in cell adhesion as well as cell-surface mediated signalling. Defects in this gene are the cause of leukocyte adhesion deficiency type I (LAD1). Two transcript variants encoding the same protein have been identified for this gene. [provided by RefSeq, Jul 2008]

UniProt Comments for ITGB2

Function: Integrin alpha-L/beta-2 is a receptor for ICAM1, ICAM2, ICAM3 and ICAM4. Integrins alpha-M/beta-2 and alpha-X/beta-2 are receptors for the iC3b fragment of the third complement component and for fibrinogen. Integrin alpha-X/beta-2 recognizes the sequence G-P-R in fibrinogen alpha-chain. Integrin alpha-M/beta-2 recognizes P1 and P2 peptides of fibrinogen gamma chain. Integrin alpha-M/beta-2 is also a receptor for factor X. Integrin alpha-D/beta-2 is a receptor for ICAM3 and VCAM1. Triggers neutrophil transmigration during lung injury through PTK2B/PYK2-mediated activation.1 PublicationManual assertion based on experiment in:Ref.15

Research Articles on ITGB2

1. Data indicate that CD47 in cis interactions regulate LFA-1 (integrin alphaLbeta2) and VLA-4 (integrin alpha4beta1) integrin affinity, and this process plays a substantial role in the adhesion of T-cells.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with ITGB2 elisa kit

Reference Product	PubMed Publications
ITGAM elisa kit	>1634 publications with ITGB2 and ITGAM
ITGAL elisa kit	>1088 publications with ITGB2 and ITGAL
ICAM1 elisa kit	>878 publications with ITGB2 and ICAM1
ITGAX elisa kit	>408 publications with ITGB2 and ITGAX
ICAM2 elisa kit	>59 publications with ITGB2 and ICAM2
ICAM3 elisa kit	>48 publications with ITGB2 and ICAM3
ITGAD elisa kit	>13 publications with ITGB2 and ITGAD
JAM3 elisa kit	>6 publications with ITGB2 and JAM3
ITGA7 elisa kit	>1 publications with ITGB2 and ITGA7

Pathways associated with ITGB2 elisa kit

Products by Pathway	Pathway Diagram
Adaptive Immune System Pathway antibodies	Adaptive Immune System Pathway Diagram
Amoebiasis Pathway antibodies	Amoebiasis Pathway Diagram
Amoebiasis Pathway antibodies	Amoebiasis Pathway Diagram
CXCR3-mediated Signaling Events Pathway antibodies	CXCR3-mediated Signaling Events Pathway Diagram
Cell Adhesion Molecules (CAMs) Pathway antibodies	Cell Adhesion Molecules (CAMs) Pathway Diagram
Cell Adhesion Molecules (CAMs) Pathway antibodies	Cell Adhesion Molecules (CAMs) Pathway Diagram
Cell Surface Interactions At The Vascular Wall Pathway antibodies	Cell Surface Interactions At The Vascular Wall Pathway Diagram
Extracellular Matrix Organization Pathway antibodies	Extracellular Matrix Organization Pathway Diagram
Focal Adhesion Pathway antibodies	Focal Adhesion Pathway Diagram
HIF-1-alpha Transcription Factor Network Pathway antibodies	HIF-1-alpha Transcription Factor Network Pathway Diagram

Diseases associated with ITGB2 elisa kit

Disease Name	Pubmed Publications
Inflammation Antibodies	>910 publications with ITGB2 and Inflammation
Necrosis Antibodies	>615 publications with ITGB2 and Necrosis
Cardiovascular Diseases Antibodies	>402 publications with ITGB2 and Cardiovascular Diseases
Disease Models, Animal Antibodies	>231 publications with ITGB2 and Disease Models, Animal
Lung Diseases Antibodies	>219 publications with ITGB2 and Lung Diseases
Leukocyte-Adhesion Deficiency Syndrome Antibodies	>165 publications with ITGB2 and Leukocyte-Adhesion Deficiency Syndrome
Heart Diseases Antibodies	>129 publications with ITGB2 and Heart Diseases
Leukocyte Disorders Antibodies	>114 publications with ITGB2 and Leukocyte Disorders
Hypersensitivity Antibodies	>112 publications with ITGB2 and Hypersensitivity
Atherosclerosis Antibodies	>72 publications with ITGB2 and Atherosclerosis

Organs/Tissues associated with ITGB2 elisa kit

Organ/Tissue Name	Pubmed Publications
Blood Antibodies	>3558 publications with ITGB2 and Blood
Lung Antibodies	>343 publications with ITGB2 and Lung
Bone Antibodies	>268 publications with ITGB2 and Bone
Bone Marrow Antibodies	>241 publications with ITGB2 and Bone Marrow
Heart Antibodies	>164 publications with ITGB2 and Heart
Muscle Antibodies	>149 publications with ITGB2 and Muscle
Embryonic Tissue Antibodies	>121 publications with ITGB2 and Embryonic Tissue
Liver Antibodies	>118 publications with ITGB2 and Liver
Spleen Antibodies	>100 publications with ITGB2 and Spleen
Intestine Antibodies	>96 publications with ITGB2 and Intestine