Anti-Nucleosome Antibody (IgG) ELISA Kit for Mouse

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with antigen. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antigen and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well and color develops in proportion to the amount of anti-nucleosome antibody (IgG) bound in the initial step. The color development is stopped and the intensity of the color is measured.

Small volumes of anti-nucleosome antibody (IgG) elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS704430 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the anti-nucleosome antibody (IgG) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing anti-nucleosome antibody (IgG). The ELISA analytical biochemical technique of the MBS704430 kit is based on anti-nucleosome antibody (IgG) antibody-anti-nucleosome antibody (IgG) antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect anti-nucleosome antibody (IgG) antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, anti-nucleosome antibody (IgG). Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).