Mucin 5b, Oligomeric Mucus/Gel-Forming (MUC5B) ELISA Kit for Human

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for MUC5B has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any MUC5B present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MUC5B is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MUC5B bound in the initial step. The color development is stopped and the intensity of the color is measured.

Small volumes of MUC5B elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS704534 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the mucin 5B, oligomeric mucus/gel-forming (MUC5B) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing MUC5B. The ELISA analytical biochemical technique of the MBS704534 kit is based on MUC5B antibody-MUC5B antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect MUC5B antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, MUC5B. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%). Mucins MUC provide protection to cells and tissues by forming thick gel-like oligomeric matrices. Mucin 5 subtype is a high molecular weight glycosylated protein expressed on the surface of tissue or cell membranes. Detection of subtype MUC5b is possible with MyBiosource's MUC5b ELISA kit. Our ELISA kits recognize only 5b oligomeric not 5ac oligomeric cell surface gel forming target samples. The specificity lies in our quality mucus antibodies, gold standard reagents such as our wash solution, detection reagent and 96 tests plate precoated with human mucin 5b oligomeric mucus antibody. Besides MUCIN 5b, we offer all ELISA kits MUCIN family from MUCIn 5ac to other cell surface ELISA kits. We recommend our webpage as your first stop for all ng ml assay needs and information on application details or target details. Transform your mucus gel forming samples into understandable proteins with all our ELISA kit MUCIN products.