Endostatin, Es (ES) ELISA Kit for Human

Name: Human ES ELISA Kit; Endostatin, Es; MyBioSource
Brand: MyBioSource
SKU: MBS704735
Price: 295 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS704735

Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for ES has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any ES present is bound by the immobilized… More details

$295 / 24-Strip-Wells (LIMIT 1) +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

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Product Overview

Online Datasheet in PDF

Product Name

Endostatin, ES, ELISA Kit

Full Product Name

Human Endostatin, ES ELISA Kit

Product Gene Name

ES elisa kit

Product Synonym Names

Human Endostatin; ES ELISA Kit; FLJ27325; FLJ34914; KNO; KNO1; MGC74745; alpha 1 type XVIII collagen; antiangiogenic agent; endostatin; multi-functional protein MFP; Endostatin; ES

Species Reactivity

Human

Assay Type

Sandwich

Samples

Serum, plasma, cell culture supernates

Detection Range

7.8 ng/ml -500 ng/ml.

Sensitivity

The minimum detectable dose of human ES is typically less than 1.95 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.

Specificity

This assay has high sensitivity and excellent specificity for detection of human ES. No significant cross-reactivity or interference between human ES and analogues was observed.

Preparation and Storage

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Inter-assay Precision

Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

Intra-assay Precision

Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.

Molecular Weight

178,188 Da

OMIM

gene 267750

3D Structure

ModBase 3D Structure for P39060

ISO Certification

Manufactured in an ISO 9001:2015 Certified Laboratory.

Other Notes

Small volumes of ES elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for ES purchase

MBS704735 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Endostatin, ES, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing ES. The ELISA analytical biochemical technique of the MBS704735 kit is based on ES antibody-ES antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect ES antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, ES. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for ES. It may not necessarily be applicable to this product.

NCBI GI #

110611235

NCBI GeneID

80781

NCBI Accession #

NP_085059.2

UniProt Primary Accession #

P39060

UniProt Secondary Accession #

Q58EX6; Q6RZ39; Q6RZ40; Q6RZ41; Q8N4S4; Q8WXI5; Q96T70; Q9UK38; Q9Y6Q7; Q9Y6Q8; A8MVI4

UniProt Related Accession #

P39060

GenBank Nucleotide #

NM_030582.3

NCBI Official Full Name

collagen alpha-1(XVIII) chain isoform 1

NCBI Official Synonym Full Names

collagen, type XVIII, alpha 1

NCBI Official Symbol

COL18A1

NCBI Official Synonym Symbols

KS; KNO; KNO1

UniProt Protein Name

Collagen alpha-1(XVIII) chain

UniProt Gene Name

COL18A1

UniProt Entry Name

COIA1_HUMAN

NCBI Protein Information

collagen alpha-1(XVIII) chain; collagen alpha-1(XVIII) chain; endostatin; antiangiogenic agent; multi-functional protein MFP

NCBI Summary for ES

This gene encodes the alpha chain of type XVIII collagen. This collagen is one of the multiplexins, extracellular matrix proteins that contain multiple triple-helix domains (collagenous domains) interrupted by non-collagenous domains. The proteolytically produced C-terminal fragment of type XVIII collagen is endostatin, a potent antiangiogenic protein. Mutations in this gene are associated with Knobloch syndrome. The main features of this syndrome involve retinal abnormalities, so type XVIII collagen may play an important role in retinal structure and in neural tube closure. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2008]

UniProt Comments for ES

Function: COLA18A probably plays a major role in determining the retinal structure as well as in the closure of the neural tube.

Research Articles on ES

1. Female type 2 diabetes mellitus patients have higher serum endostatin levels than males and exercise increases levels in both sexes.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with ES elisa kit

Products by Pathway	Pathway Diagram
Activation Of Matrix Metalloproteinases Pathway antibodies	Activation Of Matrix Metalloproteinases Pathway Diagram
Assembly Of Collagen Fibrils And Other Multimeric Structures Pathway antibodies	Assembly Of Collagen Fibrils And Other Multimeric Structures Pathway Diagram
Collagen Biosynthesis And Modifying Enzymes Pathway antibodies	Collagen Biosynthesis And Modifying Enzymes Pathway Diagram
Collagen Degradation Pathway antibodies	Collagen Degradation Pathway Diagram
Collagen Formation Pathway antibodies	Collagen Formation Pathway Diagram
Degradation Of The Extracellular Matrix Pathway antibodies	Degradation Of The Extracellular Matrix Pathway Diagram
Direct P53 Effectors Pathway antibodies	Direct P53 Effectors Pathway Diagram
Extracellular Matrix Organization Pathway antibodies	Extracellular Matrix Organization Pathway Diagram
FOXA1 Transcription Factor Network Pathway antibodies	FOXA1 Transcription Factor Network Pathway Diagram
Integrin Cell Surface Interactions Pathway antibodies	Integrin Cell Surface Interactions Pathway Diagram

Diseases associated with ES elisa kit

Disease Name	Pubmed Publications
Lung Neoplasms Antibodies	>148 publications with ES and Lung Neoplasms
Liver Neoplasms Antibodies	>118 publications with ES and Liver Neoplasms
Liver Diseases Antibodies	>114 publications with ES and Liver Diseases
Neoplasm Metastasis Antibodies	>102 publications with ES and Neoplasm Metastasis
Brain Diseases Antibodies	>68 publications with ES and Brain Diseases
Breast Neoplasms Antibodies	>53 publications with ES and Breast Neoplasms
Necrosis Antibodies	>52 publications with ES and Necrosis
Carcinoma, Hepatocellular Antibodies	>51 publications with ES and Carcinoma, Hepatocellular
Liver Neoplasms, Experimental Antibodies	>48 publications with ES and Liver Neoplasms, Experimental
Kidney Diseases Antibodies	>47 publications with ES and Kidney Diseases

Organs/Tissues associated with ES elisa kit

Organ/Tissue Name	Pubmed Publications
Vascular Antibodies	>735 publications with ES and Vascular
Blood Antibodies	>659 publications with ES and Blood
Lung Antibodies	>205 publications with ES and Lung
Liver Antibodies	>162 publications with ES and Liver
Eye Antibodies	>128 publications with ES and Eye
Embryonic Tissue Antibodies	>97 publications with ES and Embryonic Tissue
Kidney Antibodies	>80 publications with ES and Kidney
Brain Antibodies	>80 publications with ES and Brain
Muscle Antibodies	>68 publications with ES and Muscle
Bone Antibodies	>59 publications with ES and Bone