Coagulation Factor X (F10) ELISA Kit for Mouse

Name: Mouse F10 ELISA Kit; Coagulation Factor X; MyBioSource
Brand: MyBioSource
SKU: MBS704758
Price: 295 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS704758

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to FX. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for FX… More details

$295 / 24-Strip-Wells (LIMIT 1) +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Request Current Manual

Product Overview

Online Datasheet in PDF

Product Name

coagulation factor X (F10), ELISA Kit

Full Product Name

Mouse coagulation factor X, FX ELISA Kit

Product Gene Name

F10 elisa kit

Product Synonym Names

Mouse coagulation factor X; FX ELISA Kit; RP11-98F14.9; FX; FXA; Stuart-Prower factor; factor Xa; prothrombinase; coagulation factor X

Species Reactivity

Mouse

Specificity

This assay has high sensitivity and excellent specificity for detection of Mouse F10. No significant cross-reactivity or interference between Mouse F10 and analogues was observed.

Preparation and Storage

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Intra-assay Precision

Intra-assay Precision (Precision within an assay): CV% is less than 8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV% is less than 10%
Three samples of known concentration were tested in twenty assays to assess.

Molecular Weight

54,018 Da

3D Structure

ModBase 3D Structure for O88947

Detection Wavelength

450 nm

Sample Volume

50-100ul

Protein Biological Process 1

Blood Coagulation

Protein Biological Process 3

Blood coagulation

ISO Certification

Manufactured in an ISO 9001:2015 Certified Laboratory.

Other Notes

Small volumes of F10 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for F10 purchase

MBS704758 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the coagulation factor X (F10) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing F10. The ELISA analytical biochemical technique of the MBS704758 kit is based on F10 antibody-F10 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect F10 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, F10. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for F10. It may not necessarily be applicable to this product.

NCBI GI #

110625994

NCBI GeneID

14058

NCBI Accession #

NP_031998.3

UniProt Primary Accession #

O88947

UniProt Secondary Accession #

O54740; Q99L32

UniProt Related Accession #

O88947

GenBank Nucleotide #

NM_007972.4

NCBI Official Full Name

coagulation factor X isoform 2 preproprotein

NCBI Official Synonym Full Names

coagulation factor X

NCBI Official Symbol

F10

NCBI Official Synonym Symbols

fX; Cf10

UniProt Protein Name

Coagulation factor X

UniProt Synonym Protein Names

Stuart factorCleaved into the following 3 chains:Factor X light chain; Factor X heavy chain; Activated factor Xa heavy chain

Protein Family

Coagulation factor

UniProt Gene Name

F10

UniProt Entry Name

FA10_MOUSE

NCBI Protein Information

coagulation factor X; stuart factor

NCBI Summary for F10

This gene encodes factor X, a component of both the intrinsic and extrinsic blood coagulation pathways. The encoded protein is a zymogen that undergoes further processing in a vitamin K-dependent manner to generate mature factor X, a heterodimer comprised of disulfide-linked heavy and light chains. The mature factor X is proteolytically activated either by factor IXa (intrinsic pathway) or factor VIIa (extrinsic pathway) to form factor Xa serine endopeptidase. Activated factor Xa catalyzes the conversion of prothrombin to thrombin. A complete lack of the encoded protein is fatal to mice. A severe deficiency of the encoded protein in mice causes age-dependent iron deposition and cardiac fibrosis. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Aug 2015]

UniProt Comments for F10

Function: Factor Xa is a vitamin K-dependent glycoprotein that converts prothrombin to thrombin in the presence of factor Va, calcium and phospholipid during blood clotting.

Catalytic activity: Selective cleavage of Arg-|-Thr and then Arg-|-Ile bonds in prothrombin to form thrombin.

Enzyme regulation: Inhibited by SERPINA5

Research Articles on F10

1. Activated factor X signaling via protease-activated receptor 2 suppresses pro-inflammatory cytokine production from lipopolysaccharide-stimulated myeloid cells.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with F10 elisa kit

Reference Product	PubMed Publications
TFPI elisa kit	>2 publications with F10 and TFPI
PLG elisa kit	>1 publications with F10 and PLG

Pathways associated with F10 elisa kit

Products by Pathway	Pathway Diagram
Blood Clotting Cascade Pathway antibodies	Blood Clotting Cascade Pathway Diagram
Common Pathway antibodies	Common Pathway Diagram
Complement And Coagulation Cascades Pathway antibodies	Complement And Coagulation Cascades Pathway Diagram
Complement And Coagulation Cascades Pathway antibodies	Complement And Coagulation Cascades Pathway Diagram
Complement And Coagulation Cascades Pathway antibodies	Complement And Coagulation Cascades Pathway Diagram
Extrinsic Pathway antibodies	Extrinsic Pathway Diagram
Formation Of Fibrin Clot (Clotting Cascade) Pathway antibodies	Formation Of Fibrin Clot (Clotting Cascade) Pathway Diagram
Gamma-carboxylation Of Protein Precursors Pathway antibodies	Gamma-carboxylation Of Protein Precursors Pathway Diagram
Gamma-carboxylation, Transport, And Amino-terminal Cleavage Of Proteins Pathway antibodies	Gamma-carboxylation, Transport, And Amino-terminal Cleavage Of Proteins Pathway Diagram
Hemostasis Pathway antibodies	Hemostasis Pathway Diagram

Diseases associated with F10 elisa kit

Disease Name	Pubmed Publications
Lung Neoplasms Antibodies	>313 publications with F10 and Lung Neoplasms
Nervous System Diseases Antibodies	>76 publications with F10 and Nervous System Diseases
Necrosis Antibodies	>68 publications with F10 and Necrosis
Mental Disorders Antibodies	>49 publications with F10 and Mental Disorders
Liver Neoplasms Antibodies	>44 publications with F10 and Liver Neoplasms
Inflammation Antibodies	>43 publications with F10 and Inflammation
Brain Diseases Antibodies	>35 publications with F10 and Brain Diseases
Liver Neoplasms, Experimental Antibodies	>33 publications with F10 and Liver Neoplasms, Experimental
Liver Diseases Antibodies	>33 publications with F10 and Liver Diseases
Pain Antibodies	>24 publications with F10 and Pain

Organs/Tissues associated with F10 elisa kit

Organ/Tissue Name	Pubmed Publications
Blood Antibodies	>409 publications with F10 and Blood
Lung Antibodies	>391 publications with F10 and Lung
Skin Antibodies	>120 publications with F10 and Skin
Brain Antibodies	>87 publications with F10 and Brain
Liver Antibodies	>82 publications with F10 and Liver
Spleen Antibodies	>63 publications with F10 and Spleen
Embryonic Tissue Antibodies	>50 publications with F10 and Embryonic Tissue
Muscle Antibodies	>47 publications with F10 and Muscle
Kidney Antibodies	>43 publications with F10 and Kidney
Ovary Antibodies	>30 publications with F10 and Ovary