Enolase 2 (Gamma, Neuronal) (ENO2) ELISA Kit for Human

Introduction: Neuron Specific Enolase (NSE) is the most abundant form of the glycolytic enolase found in adult neurons and is thought to serve as a growth factor in neurons. Of the three enolase subunits (alpha, beta, and gamma), NSE is a dimer composed of two gamma subunits. NSE is useful in studying neuronal differentiation and is, therefore, a valuable tool for visualizing the entire neuron and endocrine systems. Serum levels of NSE have been associated with such disease states as Alzheimer's, Huntington's Chorea, neuroblastoma, head trauma, neuroendocrine malignancies, and small cell carcinomas of the lung.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to NSE. Standards or samples are then added to the appropriate microtiter plate wells with a HRP-conjugated antibody preparation specific for NSE to each microplate well and incubated. Then a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain NSE, HRP-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of NSE in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Small volumes of ENO2 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS704796 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the enolase 2 (gamma, neuronal) (ENO2) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing ENO2. The ELISA analytical biochemical technique of the MBS704796 kit is based on ENO2 antibody-ENO2 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect ENO2 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, ENO2. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).