Matrix Metallopeptidase 14 (Membrane-Inserted) (MMP14) ELISA Kit for Human

Name: Human MMP14 ELISA Kit; Matrix Metallopeptidase 14 (Membrane-Inserted); MyBioSource
Brand: MyBioSource
SKU: MBS704825
Price: 295 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS704825

Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for MMP-14 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any MMP-14 present is bound by the… More details

$295 / 24-Strip-Wells (LIMIT 1) +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

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Product Overview

Online Datasheet in PDF

Product Name

matrix metallopeptidase 14 (membrane-inserted) (MMP14), ELISA Kit

Full Product Name

Human Matrix Metalloproteinase 14, MMP-14 ELISA Kit

Product Gene Name

MMP14 elisa kit

Product Synonym Names

Human Matrix Metalloproteinase 14; MMP-14 ELISA Kit; MMP-X1; MT1-MMP; MTMMP1; matrix metalloproteinase 14; matrix metalloproteinase 14 (membrane-inserted) ; membrane type 1 metalloprotease; membrane-type matrix metalloproteinase 1; membrane-type-1 matrix m; matrix metallopeptidase 14 (membrane-inserted)

Species Reactivity

Human

Assay Type

Sandwich

Samples

Serum, plasma and tissue homogenates

Detection Range

3.12 ng/ml-200 ng/ml.

Sensitivity

The minimum detectable dose of human MMP-14 is typically less than 0.78 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.

Specificity

This assay has high sensitivity and excellent specificity for detection of human MMP-14. No significant cross-reactivity or interference between human MMP-14 and analogues was observed.

Preparation and Storage

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Inter-assay Precision

Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

Intra-assay Precision

Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.

Molecular Weight

65,894 Da

OMIM

600754

3D Structure

ModBase 3D Structure for P50281

ISO Certification

Manufactured in an ISO 9001:2015 Certified Laboratory.

Other Notes

Small volumes of MMP14 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for MMP14 purchase

MBS704825 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the matrix metallopeptidase 14 (membrane-inserted) (MMP14) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing MMP14. The ELISA analytical biochemical technique of the MBS704825 kit is based on MMP14 antibody-MMP14 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect MMP14 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, MMP14. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for MMP14. It may not necessarily be applicable to this product.

NCBI GI #

4826834

NCBI GeneID

4323

NCBI Accession #

NP_004986.1

UniProt Primary Accession #

P50281

UniProt Secondary Accession #

Q6GSF3; Q92678; A8K5L0

UniProt Related Accession #

P50281

GenBank Nucleotide #

NM_004995.3

NCBI Official Full Name

matrix metalloproteinase-14 preproprotein

NCBI Official Synonym Full Names

matrix metallopeptidase 14 (membrane-inserted)

NCBI Official Symbol

MMP14

NCBI Official Synonym Symbols

MMP-14; MMP-X1; MT-MMP; MT1MMP; MTMMP1; WNCHRS; MT1-MMP; MT-MMP 1

UniProt Protein Name

Matrix metalloproteinase-14

UniProt Synonym Protein Names

MMP-X1; Membrane-type matrix metalloproteinase 1; MT-MMP 1; MTMMP1; Membrane-type-1 matrix metalloproteinase; MT1-MMP; MT1MMP

Protein Family

Matrix metalloproteinase

UniProt Gene Name

MMP14

UniProt Synonym Gene Names

MMP-14; MT-MMP 1; MTMMP1; MT1-MMP; MT1MMP

UniProt Entry Name

MMP14_HUMAN

NCBI Protein Information

matrix metalloproteinase-14; membrane type 1 metalloprotease; membrane-type-1 matrix metalloproteinase

NCBI Summary for MMP14

Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. However, the protein encoded by this gene is a member of the membrane-type MMP (MT-MMP) subfamily; each member of this subfamily contains a potential transmembrane domain suggesting that these proteins are expressed at the cell surface rather than secreted. This protein activates MMP2 protein, and this activity may be involved in tumor invasion. [provided by RefSeq, Jul 2008]

UniProt Comments for MMP14

Function: Seems to specifically activate progelatinase A. May thus trigger invasion by tumor cells by activating progelatinase A on the tumor cell surface. May be involved in actin cytoskeleton reorganization by cleaving PTK7. Acts as a positive regulator of cell growth and migration via activation of MMP15. Involved in the formation of the fibrovascular tissues in association with pro-MMP2.3 PublicationsManual assertion based on experiment in:Ref.12

Catalytic activity: Endopeptidase activity. Activates progelatinase A by cleavage of the propeptide at 37-Asn-|-Leu-38. Other bonds hydrolyzed include 35-Gly-|-Ile-36 in the propeptide of collagenase 3, and 341-Asn-|-Phe-342, 441-Asp-|-Leu-442 and 354-Gln-|-Thr-355 in the aggrecan interglobular domain.

Cofactor: Binds 1 zinc ion per subunit

Research Articles on MMP14

1. The presence of MMP14 (and MMP2) during ovarian follicular development from the follicles and corpus luteum was confirmed.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with MMP14 elisa kit

Reference Product	PubMed Publications
MMP2 elisa kit	>139 publications with MMP14 and MMP2
MMP13 elisa kit	>50 publications with MMP14 and MMP13
TIMP2 elisa kit	>34 publications with MMP14 and TIMP2
TIMP1 elisa kit	>24 publications with MMP14 and TIMP1
FURIN elisa kit	>10 publications with MMP14 and FURIN
RECK elisa kit	>7 publications with MMP14 and RECK
FGFR2 elisa kit	>4 publications with MMP14 and FGFR2
SP1 elisa kit	>3 publications with MMP14 and SP1
COL18A1 elisa kit	>3 publications with MMP14 and COL18A1

Pathways associated with MMP14 elisa kit

Products by Pathway	Pathway Diagram
AGE/RAGE Pathway antibodies	AGE/RAGE Pathway Diagram
Activation Of Matrix Metalloproteinases Pathway antibodies	Activation Of Matrix Metalloproteinases Pathway Diagram
Collagen Degradation Pathway antibodies	Collagen Degradation Pathway Diagram
Degradation Of The Extracellular Matrix Pathway antibodies	Degradation Of The Extracellular Matrix Pathway Diagram
Extracellular Matrix Organization Pathway antibodies	Extracellular Matrix Organization Pathway Diagram
GnRH Signaling Pathway antibodies	GnRH Signaling Pathway Diagram
GnRH Signaling Pathway antibodies	GnRH Signaling Pathway Diagram
HIF-2-alpha Transcription Factor Network Pathway antibodies	HIF-2-alpha Transcription Factor Network Pathway Diagram
Matrix Metalloproteinases Pathway antibodies	Matrix Metalloproteinases Pathway Diagram
Senescence And Autophagy Pathway antibodies	Senescence And Autophagy Pathway Diagram

Diseases associated with MMP14 elisa kit

Disease Name	Pubmed Publications
Neoplasm Invasiveness Antibodies	>109 publications with MMP14 and Neoplasm Invasiveness
Neoplasm Metastasis Antibodies	>82 publications with MMP14 and Neoplasm Metastasis
Breast Neoplasms Antibodies	>60 publications with MMP14 and Breast Neoplasms
Disease Models, Animal Antibodies	>54 publications with MMP14 and Disease Models, Animal
Adenocarcinoma Antibodies	>46 publications with MMP14 and Adenocarcinoma
Inflammation Antibodies	>38 publications with MMP14 and Inflammation
Cardiovascular Diseases Antibodies	>34 publications with MMP14 and Cardiovascular Diseases
Lung Diseases Antibodies	>29 publications with MMP14 and Lung Diseases
Lung Neoplasms Antibodies	>25 publications with MMP14 and Lung Neoplasms
Fibrosis Antibodies	>23 publications with MMP14 and Fibrosis

Organs/Tissues associated with MMP14 elisa kit

Organ/Tissue Name	Pubmed Publications
Vascular Antibodies	>74 publications with MMP14 and Vascular
Blood Antibodies	>64 publications with MMP14 and Blood
Bone Antibodies	>48 publications with MMP14 and Bone
Lung Antibodies	>39 publications with MMP14 and Lung
Muscle Antibodies	>37 publications with MMP14 and Muscle
Embryonic Tissue Antibodies	>28 publications with MMP14 and Embryonic Tissue
Connective Tissue Antibodies	>26 publications with MMP14 and Connective Tissue
Brain Antibodies	>20 publications with MMP14 and Brain
Skin Antibodies	>18 publications with MMP14 and Skin
Mouth Antibodies	>16 publications with MMP14 and Mouth