Sparc/Osteonectin, Cwcv and Kazal-Like Domains Proteoglycan (Testican) 2 (SPOCK2) ELISA Kit for Human

Name: Human SPOCK2 ELISA Kit; MyBioSource
Brand: MyBioSource
SKU: MBS704829
Price: 310 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS704829

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for ON has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any ON present is bound by the immobilized… More details

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Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

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Product Overview

Product Name

sparc/osteonectin, cwcv and kazal-like domains proteoglycan (testican) 2 (SPOCK2), ELISA Kit

Full Product Name

Human osteonectin, ON ELISA Kit

Product Gene Name

SPOCK2 elisa kit

Product Synonym Names

Human osteonectin; ON ELISA Kit; FLJ97039; testican-2; ; sparc/osteonectin; cwcv and kazal-like domains proteoglycan (testican) 2

Species Reactivity

Human

Assay Type

Sandwich

Samples

Serum, plasma, saliva and tissue homogenates

Detection Range

0.78 ng/ml-50 ng/ml

Sensitivity

0.195 ng/ml

Specificity

This assay has high sensitivity and excellent specificity for detection of Human SPOCK2. No significant cross-reactivity or interference between Human SPOCK2 and analogues was observed.

Preparation and Storage

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Intra-assay Precision

Intra-assay Precision (Precision within an assay): CV% is less than 8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV% is less than 10%
Three samples of known concentration were tested in twenty assays to assess.

Molecular Weight

46,779 Da

OMIM

607988

3D Structure

ModBase 3D Structure for Q92563

Detection Wavelength

450 nm

Sample Volume

50-100ul

ISO Certification

Manufactured in an ISO 9001:2015 Certified Laboratory.

Other Notes

Small volumes of SPOCK2 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for SPOCK2 purchase

MBS704829 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the sparc/osteonectin, cwcv and kazal-like domains proteoglycan (testican) 2 (SPOCK2) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing SPOCK2. The ELISA analytical biochemical technique of the MBS704829 kit is based on SPOCK2 antibody-SPOCK2 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect SPOCK2 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, SPOCK2. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for SPOCK2. It may not necessarily be applicable to this product.

NCBI GI #

197313714

NCBI GeneID

9806

NCBI Accession #

NP_001127906.1

UniProt Primary Accession #

Q92563

UniProt Secondary Accession #

Q6UW87; C9J767

UniProt Related Accession #

Q92563

GenBank Nucleotide #

NM_001134434.1

NCBI Official Full Name

testican-2 isoform 1

NCBI Official Synonym Full Names

sparc/osteonectin, cwcv and kazal-like domains proteoglycan (testican) 2

NCBI Official Symbol

SPOCK2

NCBI Official Synonym Symbols

testican-2

UniProt Protein Name

Testican-2

UniProt Synonym Protein Names

SPARC/osteonectin, CWCV, and Kazal-like domains proteoglycan 2

Protein Family

Testican

UniProt Gene Name

SPOCK2

UniProt Synonym Gene Names

KIAA0275; TICN2; UNQ269/PRO306

UniProt Entry Name

TICN2_HUMAN

NCBI Protein Information

testican-2; SPARC/osteonectin, CWCV, and Kazal-like domains proteoglycan 2

NCBI Summary for SPOCK2

This gene encodes a protein which binds with glycosaminoglycans to form part of the extracellular matrix. The protein contains thyroglobulin type-1, follistatin-like, and calcium-binding domains, and has glycosaminoglycan attachment sites in the acidic C-terminal region. Three alternatively spliced transcript variants that encode different protein isoforms have been described for this gene. [provided by RefSeq, Oct 2011]

UniProt Comments for SPOCK2

Function: May participate in diverse steps of neurogenesis. Binds calcium.

Research Articles on SPOCK2

1. candidate susceptibility gene for bronchopulmonary dysplasia; lung expression pattern indicates potential role in alveolarization

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with SPOCK2 elisa kit

Reference Product	PubMed Publications
SPARC elisa kit	>5 publications with SPOCK2 and SPARC
SPOCK3 elisa kit	>3 publications with SPOCK2 and SPOCK3
MMP16 elisa kit	>3 publications with SPOCK2 and MMP16
TES elisa kit	>2 publications with SPOCK2 and TES

Pathways associated with SPOCK2 elisa kit

Products by Pathway	Pathway Diagram
Hemostasis Pathway antibodies	Hemostasis Pathway Diagram
Platelet Activation, Signaling And Aggregation Pathway antibodies	Platelet Activation, Signaling And Aggregation Pathway Diagram
Platelet Degranulation Pathway antibodies	Platelet Degranulation Pathway Diagram
Response To Elevated Platelet Cytosolic Ca2+ Pathway antibodies	Response To Elevated Platelet Cytosolic Ca2+ Pathway Diagram
Senescence And Autophagy Pathway antibodies	Senescence And Autophagy Pathway Diagram
TGF-beta Receptor Signaling Pathway antibodies	TGF-beta Receptor Signaling Pathway Diagram

Diseases associated with SPOCK2 elisa kit

Disease Name	Pubmed Publications
Breast Neoplasms Antibodies	>2 publications with SPOCK2 and Breast Neoplasms
Genital Diseases, Male Antibodies	>1 publications with SPOCK2 and Genital Diseases, Male
Chromosome Aberrations Antibodies	>1 publications with SPOCK2 and Chromosome Aberrations
Prostatic Diseases Antibodies	>1 publications with SPOCK2 and Prostatic Diseases
Inflammation Antibodies	>1 publications with SPOCK2 and Inflammation
Necrosis Antibodies	>1 publications with SPOCK2 and Necrosis

Organs/Tissues associated with SPOCK2 elisa kit

Organ/Tissue Name	Pubmed Publications
Bone Antibodies	>947 publications with SPOCK2 and Bone
Connective Tissue Antibodies	>408 publications with SPOCK2 and Connective Tissue
Blood Antibodies	>288 publications with SPOCK2 and Blood
Vascular Antibodies	>259 publications with SPOCK2 and Vascular
Bone Marrow Antibodies	>174 publications with SPOCK2 and Bone Marrow
Embryonic Tissue Antibodies	>155 publications with SPOCK2 and Embryonic Tissue
Muscle Antibodies	>148 publications with SPOCK2 and Muscle
Mouth Antibodies	>140 publications with SPOCK2 and Mouth
Brain Antibodies	>121 publications with SPOCK2 and Brain
Skin Antibodies	>104 publications with SPOCK2 and Skin