Milk Fat Globule-EGF Factor 8 (MFGE8) ELISA Kit for Human

For Sample:For the quantitative determination of human milk fat globule-EGF factor 8 protein (MFGE8) concentrations in serum, cell culture supernates, urine, cerebrospinal fluid (CSF).

PRINCIPLE OF THE ASSAY This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for MFGE8 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any MFGE8 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MFGE8 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MFGE8 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Small volumes of MFGE8 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS705026 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the milk fat globule-EGF factor 8 protein (MFGE8) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing MFGE8. The ELISA analytical biochemical technique of the MBS705026 kit is based on MFGE8 antibody-MFGE8 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect MFGE8 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, MFGE8. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).