Free Tri-Iodothyronine Indes, Free-T3 (FT3) ELISA Kit for Mouse

Principle of the Assay: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to T3. Standards or samples are added to the appropriate microtiter plate wells with Biotin-conjugated T3. A competitive inhibition reaction is launched between T3 (Standards or samples) and Biotin-conjugated T3 with the pre-coated antibody specific for T3. The more amount of T3 in samples, the less antibody bound by Biotin-conjugated T3. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Substrate solution is added to the wells and the color develops in opposite to the amount of Free-T3 in the sample. The color development is stopped and the intensity of the color is measured.

Small volumes of FT3 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS705057 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Free Tri-iodothyronine Indes, Free-T3 (FT3) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing FT3. The ELISA analytical biochemical technique of the MBS705057 kit is based on FT3 antibody-FT3 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect FT3 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, FT3. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).