Apolipoprotein B48 (apo-B48) ELISA Kit for Mouse

Principle of the Assay: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with apo-B48. Standards or samples are added to the appropriate microtiter plate wells with Horseradish Peroxidase (HRP) conjugated antibody preparation specific for apo-B48. The competitive inhibition reaction is launched between with pre-coated apo-B48 and apo-B48 in samples. A substrate solution is added to the wells and the color develops in opposite to the amount of apo-B48 in the samples. The color development is stopped and the intensity of the color is measured.

Small volumes of apo-B48 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS705984 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Apolipoprotein B48 (apo-B48) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing apo-B48. The ELISA analytical biochemical technique of the MBS705984 kit is based on apo-B48 antibody-apo-B48 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect apo-B48 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, apo-B48. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).