Heparin (Hep) Antibody (IgM) ELISA Kit for Human

For samples: For the qualitative determination of human heparin (Hep) antibody (IgM) concentrations in serum, plasma.

PRINCIPLE OF THE ASSAY This assay employs the qualitative enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with antigen. Samples are pipetted into the wells with anti-human IgM conjugated Horseradish Peroxidase (HRP). Any antibodies specific for the antigen present will bind to the pre-coated antigen. Following a wash to remove any unbound reagent, a substrate solution is added to the wells and color develops in proportion to the amount of human heparin (Hep) antibody (IgM) bound in the initial step. The color development is stopped and the intensity of the color is measured.

Small volumes of heparin (Hep) antibody (IgM) elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS706452 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the heparin (Hep) antibody (IgM) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing heparin (Hep) antibody (IgM). The ELISA analytical biochemical technique of the MBS706452 kit is based on heparin (Hep) antibody (IgM) antibody-heparin (Hep) antibody (IgM) antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect heparin (Hep) antibody (IgM) antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, heparin (Hep) antibody (IgM). Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).