Bovine TNF Recombinant Protein – Cat# MBS717150

Tumor Necrosis Factor (TNF) Bovine Recombinant Protein

Name: TNF Recombinant Protein; Tumor Necrosis Factor; Bovine; MyBioSource
Brand: MyBioSource
SKU: MBS717150
Price: 255 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS717150

Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is… More details

$255 / 0.01 mg +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

Tumor necrosis factor (TNF), Recombinant Protein

Full Product Name

Recombinant Bovine Tumor necrosis factor protein

Product Gene Name

TNF recombinant protein

Product Synonym Names

Tumor necrosis factor His tagged; Cachectin; TNF-alpha; Tumor necrosis factor ligand superfamily member 2; TNF-a

Sequence

LRSSSQASSN KPVAHVVADI NSPGQLRWWD SYANALMANG VKLEDNQLVV PADGLYLIYS QVLFRGQGCP STPLFLTHTI SRIAVSYQTK VNILSAIKSP CHRETPEWAE AKPWYEPIYQ GGVFQLEKGD RLSAEINLPD YLDYAESGQV YFGIIAL

Host

E Coli

Form/Format

Liquid containing glycerol

Purity/Purification

Greater or equal to 85% purity as determined by SDS-PAGE. (lot specific)

Preparation and Storage

Store working aliquots at 4 degree C for up to one week. For extended storage, conserve at -20 degree C or -80 degree C. Repeated freezing and thawing is not recommended.

Molecular Weight

23 KD

Tag Information

This protein contains an N-terminal tag and may also contain a C-terminal tag. Tag types are determined by various factors including tag-protein stability, please inquire for tag information.

Sterility

Sterile filter available upon request.

Endotoxin

Low endotoxin available upon request.

Chromosome Location

Chromosome: 23; NC_007324.4 (27486264..27489037, complement). Location: 23q22

3D Structure

ModBase 3D Structure for Q06599

ISO Certification

Manufactured in an ISO 9001:2015 Certified Laboratory.

Applications Tested/Suitable for TNF recombinant protein

SDS-PAGE, ELISA

Product References and Citations for TNF recombinant protein

[1] "Cloning of two members of the TNF-superfamily in cattle: CD40 ligand and tumor necrosis factor alpha." Mertens B.E.L.C., Muriuki M., Gaidulis L. Immunogenetics 42:430-431(1995) [PubMed: 7590981] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 50-233 (ISOFORM 1). Tissue: Blood.
[2] "Rapid communication: single strand conformational polymorphism (SSCP) of bovine tumor necrosis factor alpha." Dietz A.B., Neibergs H.L., Womack J.E., Kehrli M.E. Jr. J. Anim. Sci. 75:2567-2567(1997) [PubMed: 9303477] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 91-193. Strain: Holstein.

Other Notes

Small volumes of TNF recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for TNF purchase

MBS717150 is a recombinant protein targeting TNF. Also known as Recombinant Bovine Tumor necrosis factor protein. Host: E Coli.

Additional Details

NCBI/Uniprot data below describe general gene information for TNF. It may not necessarily be applicable to this product.

NCBI GI #

145279648

NCBI GeneID

280943

NCBI Accession #

NP_776391.2

UniProt Primary Accession #

Q06599

UniProt Secondary Accession #

O18779; Q27978; A4IFT5; A9QWR7

UniProt Related Accession #

Q06599

GenBank Nucleotide #

NM_173966.2

NCBI Official Full Name

Bos taurus tumor necrosis factor (TNF), mRNA

NCBI Official Symbol

TNF

NCBI Official Synonym Symbols

TNFa

UniProt Protein Name

Tumor necrosis factor

UniProt Synonym Protein Names

Cachectin; TNF-alpha; Tumor necrosis factor ligand superfamily member 2

Protein Family

Tumor necrosis factor

UniProt Gene Name

TNF

UniProt Synonym Gene Names

TNFA; TNFSF2

UniProt Entry Name

TNFA_BOVIN

NCBI Protein Information

tumor necrosis factor; TNF-a; TNF-alpha; cachectin; tumor necrosis factor alpha; tumor necrosis factor (TNF superfamily, member 2); tumor necrosis factor ligand superfamily member 2; tumor necrosis factor, alpha (TNF superfamily, member 2)

UniProt Comments for TNF

Function: Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation.Subunit structure: Homotrimer By similarity.Subcellular location: Cell membrane; Single-pass type II membrane protein By similarity. Tumor necrosis factor, soluble form: Secreted By similarity. Post-translational modification: The soluble form derives from the membrane form by proteolytic processing By similarity.The membrane form, but not the soluble form, is phosphorylated on serine residues. Dephosphorylation of the membrane form occurs by binding to soluble TNFRSF1A/TNFR1 By similarity.Sequence similarities: Belongs to the tumor necrosis factor family.Sequence caution: The sequence AAA19573.1 differs from that shown. Reason: Erroneous gene model prediction.

Research Articles on TNF

1. Results indicate that TNF-alpha does not affect autonomous, pulsatile progesterone (P(4)) release, increases P(4) secretion by bovine corpus luteum (CL) with increasing dose & reduces in a dose-dependent manner responsiveness of CL to luteotropic f...

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with TNF recombinant protein

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Adipocytokine Signaling Pathway antibodies	Adipocytokine Signaling Pathway Diagram
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African Trypanosomiasis Pathway antibodies	African Trypanosomiasis Pathway Diagram
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Allograft Rejection Pathway antibodies	Allograft Rejection Pathway Diagram
Allograft Rejection Pathway antibodies	Allograft Rejection Pathway Diagram
Alzheimer's Disease Pathway antibodies	Alzheimer's Disease Pathway Diagram
Alzheimer's Disease Pathway antibodies	Alzheimer's Disease Pathway Diagram
Amoebiasis Pathway antibodies	Amoebiasis Pathway Diagram
Amoebiasis Pathway antibodies	Amoebiasis Pathway Diagram

Diseases associated with TNF recombinant protein

Disease Name	Pubmed Publications
Inflammation Antibodies	>21554 publications with TNF and Inflammation
Infection Antibodies	>12856 publications with TNF and Infection
Neoplasms Antibodies	>11768 publications with TNF and Neoplasms
Cardiovascular Diseases Antibodies	>6129 publications with TNF and Cardiovascular Diseases
Lung Diseases Antibodies	>4975 publications with TNF and Lung Diseases
Arthritis, Rheumatoid Antibodies	>4945 publications with TNF and Arthritis, Rheumatoid
Sepsis Antibodies	>4146 publications with TNF and Sepsis
Skin Diseases Antibodies	>4090 publications with TNF and Skin Diseases
Liver Diseases Antibodies	>3627 publications with TNF and Liver Diseases
Leukemia Antibodies	>2497 publications with TNF and Leukemia

Organs/Tissues associated with TNF recombinant protein

Organ/Tissue Name	Pubmed Publications
Intestine Antibodies	>2561 publications with TNF and Intestine
Pancreas Antibodies	>863 publications with TNF and Pancreas
Uncharacterized Tissue Antibodies	>13 publications with TNF and Uncharacterized Tissue
Lymphoreticular Antibodies	>9 publications with TNF and Lymphoreticular