IL4 Recombinant Protein – Cat# MBS717398

Interleukin-4 (IL4) Recombinant Protein

Name: IL4 Recombinant Protein; Interleukin-4; Research Grade; MyBioSource
Brand: MyBioSource
SKU: MBS717398
Price: 255 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS717398

Participates in at least several B-cell activation processes as well as of other cell types. It is a costimulator of DNA-synthesis. It induces the expression of class II MHC molecules on resting B-cells. It enhances both secretion and cell surface expression… More details

$255 / 0.01 mg +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

Interleukin-4 (IL4), Recombinant Protein

Full Product Name

Recombinant human Interleukin-4 protein

Product Gene Name

IL4 recombinant protein

Product Synonym Names

Interleukin-4 His tagged; B-cell stimulatory factor 1; BSF-1; Binetrakin; Lymphocyte stimulatory factor 1; Pitrakinra

Sequence

HKCDITLQEI IKTLNSLTEQ KTLCTELTVT DIFAASKNTT EKETFCRAAT VLRQFYSHHE KDTRCLGATA QQFHRHKQLI RFLKRLDRNL WGLAGLNSCP VKEANQSTLE NFLERLKTIM REKYSKCSS-

Host

E Coli

Form/Format

Liquid containing glycerol

Purity/Purification

Greater or equal to 85% purity as determined by SDS-PAGE. (lot specific)

Preparation and Storage

Store working aliquots at 4 degree C for up to one week. For extended storage, conserve at -20 degree C or -80 degree C. Repeated freezing and thawing is not recommended.

Molecular Weight

41 kDa

Tag Information

This protein contains an N-terminal tag and may also contain a C-terminal tag. Tag types are determined by various factors including tag-protein stability, please inquire for tag information.

Sterility

Sterile filter available upon request.

Endotoxin

Low endotoxin available upon request.

Chromosome Location

Chromosome: 5; NC_000005.9 (132009678..132018370). Location: 5q31.1

OMIM

147780

3D Structure

ModBase 3D Structure for P05112

ISO Certification

Manufactured in an ISO 9001:2015 Certified Laboratory.

Applications Tested/Suitable for IL4 recombinant protein

SDS-PAGE, ELISA

Product References and Citations for IL4 recombinant protein

[1] "Isolation and characterization of a human interleukin cDNA clone, homologous to mouse B-cell stimulatory factor 1, that expresses B-cell- and T-cell-stimulating activities." Yokota T., Otsuka T., Mosmann T., Banchereau J., Defrance T., Blanchard D., de Vries J.E., Lee F., Arai K. Proc. Natl. Acad. Sci. U.S.A. 83:5894-5898(1986) [PubMed: 3016727] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
[2] "Complete nucleotide sequence of the chromosomal gene for human IL-4 and its expression." Arai N., Nomura D., Villaret D., Malefijt R.D., Seiki M., Yoshida M., Minoshima S., Fukuyama R., Maekawa M., Kudoh J., Shimizu N., Yokota K., Abe E., Yokota T., Takebe Y., Arai K. J. Immunol. 142:274-282(1989) [PubMed: 2535858] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3] "An alternatively spliced interleukin 4 form in lymphoid cells." Klein S.C., Golverdingen J., Bouwens A.G.M., Tilanus M.G.J. Immunogenetics 41:57-57(1995) [PubMed: 7806280] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
[4] SeattleSNPs variation discovery resource Submitted (JUN-2001) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[5] "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Blood.

Other Notes

Small volumes of IL4 recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for IL4 purchase

MBS717398 is a recombinant protein targeting IL4. Also known as Recombinant human Interleukin-4 protein. Host: E Coli.

Additional Details

NCBI/Uniprot data below describe general gene information for IL4. It may not necessarily be applicable to this product.

NCBI GI #

4504669

NCBI GeneID

3565

NCBI Accession #

NP_000580.1

UniProt Primary Accession #

P05112

UniProt Secondary Accession #

Q14630

UniProt Related Accession #

P05112

GenBank Nucleotide #

NM_000589.3

NCBI Official Full Name

interleukin-4 isoform 1

NCBI Official Synonym Full Names

interleukin 4

NCBI Official Symbol

IL4

NCBI Official Synonym Symbols

BSF1; IL-4; BCGF1; BSF-1; BCGF-1

UniProt Protein Name

Interleukin-4

UniProt Synonym Protein Names

B-cell stimulatory factor 1; BSF-1; Binetrakin; Lymphocyte stimulatory factor 1; Pitrakinra

Protein Family

Interleukin

UniProt Gene Name

IL4

UniProt Synonym Gene Names

IL-4; BSF-1

UniProt Entry Name

IL4_HUMAN

NCBI Protein Information

interleukin-4; binetrakin; pitrakinra; B cell growth factor 1; B_cell stimulatory factor 1; lymphocyte stimulatory factor 1

NCBI Summary for IL4

The protein encoded by this gene is a pleiotropic cytokine produced by activated T cells. This cytokine is a ligand for interleukin 4 receptor. The interleukin 4 receptor also binds to IL13, which may contribute to many overlapping functions of this cytokine and IL13. STAT6, a signal transducer and activator of transcription, has been shown to play a central role in mediating the immune regulatory signal of this cytokine. This gene, IL3, IL5, IL13, and CSF2 form a cytokine gene cluster on chromosome 5q, with this gene particularly close to IL13. This gene, IL13 and IL5 are found to be regulated coordinately by several long-range regulatory elements in an over 120 kilobase range on the chromosome. Two alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported. [provided by RefSeq, Jul 2008]

UniProt Comments for IL4

Function: Participates in at least several B-cell activation processes as well as of other cell types. It is a costimulator of DNA-synthesis. It induces the expression of class II MHC molecules on resting B-cells. It enhances both secretion and cell surface expression of IgE and IgG1. It also regulates the expression of the low affinity Fc receptor for IgE (CD23) on both lymphocytes and monocytes.

Subcellular location: Secreted.

Involvement in disease: Ischemic stroke (ISCHSTR) [MIM:601367]: A stroke is an acute neurologic event leading to death of neural tissue of the brain and resulting in loss of motor, sensory and/or cognitive function. Ischemic strokes, resulting from vascular occlusion, is considered to be a highly complex disease consisting of a group of heterogeneous disorders with multiple genetic and environmental risk factors.Note: Disease susceptibility is associated with variations affecting the gene represented in this entry. Ref.19

Sequence similarities: Belongs to the IL-4/IL-13 family.

Research Articles on IL4

1. IL-9-producing invariant NKT cells protect against DSS-induced colitis in an IL-4-dependent manner.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with IL4 recombinant protein

Reference Product	PubMed Publications
IL5 recombinant protein	>183 publications with IL4 and IL5
STAT6 recombinant protein	>89 publications with IL4 and STAT6
IL4R recombinant protein	>63 publications with IL4 and IL4R
IL13RA1 recombinant protein	>15 publications with IL4 and IL13RA1
CSF2 recombinant protein	>14 publications with IL4 and CSF2
JAK2 recombinant protein	>10 publications with IL4 and JAK2
JAK3 recombinant protein	>8 publications with IL4 and JAK3
IL2RG recombinant protein	>3 publications with IL4 and IL2RG

Pathways associated with IL4 recombinant protein

Products by Pathway	Pathway Diagram
Allograft Rejection Pathway antibodies	Allograft Rejection Pathway Diagram
Allograft Rejection Pathway antibodies	Allograft Rejection Pathway Diagram
Asthma Pathway antibodies	Asthma Pathway Diagram
Asthma Pathway antibodies	Asthma Pathway Diagram
Autoimmune Thyroid Disease Pathway antibodies	Autoimmune Thyroid Disease Pathway Diagram
Autoimmune Thyroid Disease Pathway antibodies	Autoimmune Thyroid Disease Pathway Diagram
CD40/CD40L Signaling Pathway antibodies	CD40/CD40L Signaling Pathway Diagram
Calcineurin-regulated NFAT-dependent Transcription In Lymphocytes Pathway antibodies	Calcineurin-regulated NFAT-dependent Transcription In Lymphocytes Pathway Diagram
Calcium Signaling In The CD4+ TCR Pathway antibodies	Calcium Signaling In The CD4+ TCR Pathway Diagram
Cytokine-cytokine Receptor Interaction Pathway antibodies	Cytokine-cytokine Receptor Interaction Pathway Diagram

Diseases associated with IL4 recombinant protein

Disease Name	Pubmed Publications
Necrosis Antibodies	>275 publications with IL4 and Necrosis
Hypersensitivity Antibodies	>268 publications with IL4 and Hypersensitivity
Inflammation Antibodies	>262 publications with IL4 and Inflammation
Hypersensitivity, Immediate Antibodies	>214 publications with IL4 and Hypersensitivity, Immediate
Asthma Antibodies	>188 publications with IL4 and Asthma
Lung Diseases Antibodies	>178 publications with IL4 and Lung Diseases
Respiratory Hypersensitivity Antibodies	>161 publications with IL4 and Respiratory Hypersensitivity
Autoimmune Diseases Antibodies	>157 publications with IL4 and Autoimmune Diseases
Liver Diseases Antibodies	>47 publications with IL4 and Liver Diseases
Cardiovascular Diseases Antibodies	>46 publications with IL4 and Cardiovascular Diseases

Organs/Tissues associated with IL4 recombinant protein

Organ/Tissue Name	Pubmed Publications
Blood Antibodies	>1117 publications with IL4 and Blood
Spleen Antibodies	>96 publications with IL4 and Spleen