30s Ribosomal Protein S4 (rpsD) E Coli Recombinant Protein

One of two assembly initiator proteins for the 30S subunit, it binds directly to 16S rRNA where it nucleates assembly of the body of the 30S subunit. With S5 and S12 plays an important role in translational accuracy; many suppressors of streptomycin-dependent mutants of protein S12 are found in this protein, some but not all of which decrease translational accuracy (ram, ribosomal ambiguity mutations). Plays a role in mRNA unwinding by the ribosome, possibly by forming part of a processivity clamp. Protein S4 is also a translational repressor protein, it controls the translation of the alpha-operon (which codes for S13, S11, S4, RNA polymerase alpha subunit, and L17) by binding to its mRNA. Also functions as a rho-dependent antiterminator of rRNA transcription, increasing the synthesis of rRNA under conditions of excess protein, allowing a more rapid return to homeostasis. Binds directly to RNA polymerase.

Nucleotide sequence of the alpha ribosomal protein operon of Escherichia coli.Bedwell D.M., Davis G.R., Gosink M., Post L.E., Nomura M., Kestler H., Zengel J.M., Lindahl L.Nucleic Acids Res. 13:3891-3903(1985) The complete genome sequence of Escherichia coli K-12.Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y.Science 277:1453-1462(1997) Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110.Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.Mol. Syst. Biol. 2:E1-E5(2006) The primary structure of ribosomal protein S4 from Escherichia coli.Reinbolt J., Schiltz E.FEBS Lett. 36:250-252(1973) Determination of the complete amino-acid sequence of protein S4 from Escherichia coli ribosomes.Schiltz E., Reinbolt J.Eur. J. Biochem. 56:467-481(1975) Protein-rRNA binding features and their structural and functional implications in ribosomes as determined by cross-linking studies.Urlaub H., Kruft V., Bischof O., Mueller E.-C., Wittmann-Liebold B.EMBO J. 14:4578-4588(1995) Bienvenut W.V., Barblan J., Quadroni M.Submitted (JAN-2004) to UniProtKBNucleotide sequence of the intercistronic region preceding the gene for RNA polymerase subunit alpha in Escherichia coli.Post L.E., Nomura M.J. Biol. Chem. 254:10604-10606(1979) Regulation of alpha operon gene expression in Escherichia coli. A novel form of translational coupling.Thomas M.S., Bedwell D.M., Nomura M.J. Mol. Biol. 196:333-345(1987) Assembly of the 30S subunit from Escherichia coli ribosomes occurs via two assembly domains which are initiated by S4 and S7.Nowotny V., Nierhaus K.H.Biochemistry 27:7051-7055(1988) Mutations in ribosomal proteins S4 and S12 influence the higher order structure of 16 S ribosomal RNA.Allen P.N., Noller H.F.J. Mol. Biol. 208:457-468(1989) Messenger RNA recognition by fragments of ribosomal protein S4.Baker A.M., Draper D.E.J. Biol. Chem. 270:22939-22945(1995) Flexibility of the nascent polypeptide chain within the ribosome --contacts from the peptide N-terminus to a specific region of the 30S subunit.Choi K.M., Atkins J.F., Gesteland R.F., Brimacombe R.Eur. J. Biochem. 255:409-413(1998) A novel mutation in ribosomal protein S4 that affects the function of a mutated RF1.Dahlgren A., Ryden-Aulin M.Biochimie 82:683-691(2000) Ribosomal protein S4 is a transcription factor with properties remarkably similar to NusA, a protein involved in both non-ribosomal and ribosomal RNA antitermination.Torres M., Condon C., Balada J.-M., Squires C., Squires C.L.EMBO J. 20:3811-3820(2001) mRNA helicase activity of the ribosome.Takyar S., Hickerson R.P., Noller H.F.Cell 120:49-58(2005) Observation of Escherichia coli ribosomal proteins and their posttranslational modifications by mass spectrometry.Arnold R.J., Reilly J.P.Anal. Biochem. 269:105-112(1999) All-atom homology model of the Escherichia coli 30S ribosomal subunit.Tung C.-S., Joseph S., Sanbonmatsu K.Y.Nat. Struct. Biol. 9:750-755(2002) Study of the structural dynamics of the E. coli 70S ribosome using real-space refinement.Gao H., Sengupta J., Valle M., Korostelev A., Eswar N., Stagg S.M., Van Roey P., Agrawal R.K., Harvey S.C., Sali A., Chapman M.S., Frank J.Cell 113:789-801(2003) Limitations of translational accuracy.Kurland C.G., Hughes D., Ehrenberg M.(In) Neidhardt F.C., Curtiss R. III, Ingraham J.L., Lin E.C.C., Low K.B. Magasanik B., Reznikoff W.S., Riley M., Schaechter M., Umbarger H.E. (eds.) ;Escherichia coli and Salmonella Cellular and molecular biology (2nd ed.) , pp.979-1004, American Society for Microbiology Press, Washington D.C. (1996) Structures of the bacterial ribosome at 3.5 A resolution.Schuwirth B.S., Borovinskaya M.A., Hau C.W., Zhang W., Vila-Sanjurjo A., Holton J.M., Cate J.H.D.Science 310:827-834(2005)

Small volumes of rpsD recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS717421 is a recombinant protein targeting rpsD. Also known as Recombinant E Coli 30S ribosomal protein S4. Host: E Coli.