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Keratin, Type II Cytoskeletal 4 (KRT4) Human Recombinant Protein

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS717756

MBS717756 is a recombinant protein targeting KRT4. Also known as Recombinant Human Keratin, type II cytoskeletal 4. Host: E Coli or Yeast or Baculovirus or Mammalian Cell. More details

$315 / 0.02 mg (E-Coli) +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Keratin, type II cytoskeletal 4 (KRT4), Recombinant Protein
Full Product Name
Recombinant Human Keratin, type II cytoskeletal 4
Product Gene Name
Product Synonym Names
Cytokeratin-4; CK-4; Keratin-4; K4; Type-II keratin Kb4
Sequence
MIARQQCVRG GPRGFSCGSA IVGGGKRGAF SSVSMSGGAG RCSSGGFGSR SLYNLRGNKS ISMSVAGSRQ GACFGGAGGF GTGGFGAGGF GAGFGTGGFG GGFGGSFSGK GGPGFPVCPA GGIQEVTINQ SLLTPLHVEI DPEIQKVRTE EREQIKLLNN KFASFIDKVQ FLEQQNKVLE TKWNLLQQQT TTTSSKNLEP LFETYLSVLR KQLDTLGNDK GRLQSELKTM QDSVEDFKTK YEEEINKRTA AENDFVVLKK DVDAAYLNKV ELEAKVDSLN DEINFLKVLY DAELSQMQTH VSDTSVVLSM DNNRNLDLDS IIAEVRAQYE EIAQRSKAEA EALYQTKVQQ LQISVDQHGD NLKNTKSEIA ELNRMIQRLR AEIENIKKQC QTLQVSVADA EQRGENALKD AHSKRVELEA ALQQAKEELA RMLREYQELM SVKLALDIEI ATYRKLLEGE EYRMSGECQS AVSISVVSGS TSTGGISGGL GSGSGFGLSS GFGSGSGSGF GFGGSVSGSS SSKIISTTTL NKRR
Host
E Coli or Yeast or Baculovirus or Mammalian Cell
Form/Format
Lyophilized or liquid (Format to be determined during the manufacturing process)
Purity/Purification
Greater or equal to 85% purity as determined by SDS-PAGE. (lot specific)
Preparation and Storage
Store at -20 degree C, for extended storage, conserve at -20 degree C or -80 degree C.
Molecular Weight
59.3 kDa
Sequence Positions
1-534aa; Full Length (OLD version: https://www.uniprot.org/uniprot/P19013.txt?version=185)
Tag Information
This protein contains an N-terminal tag and may also contain a C-terminal tag. Tag types are determined by various factors including tag-protein stability, please inquire for tag information.
Sterility
Sterile filter available upon request.
Endotoxin
Low endotoxin available upon request.
OMIM
123940
3D Structure
ModBase 3D Structure for P19013

Product Categories/Family for KRT4 recombinant protein

ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.

Product References and Citations for KRT4 recombinant protein

A mutation detection strategy for oral mucosal keratins K4, K13, and K2p in white sponge nevus.Cassidy A.J., Morley S.M., McLean W.H.I.The finished DNA sequence of human chromosome 12.Scherer S.E., Muzny D.M., Buhay C.J., Chen R., Cree A., Ding Y., Dugan-Rocha S., Gill R., Gunaratne P., Harris R.A., Hawes A.C., Hernandez J., Hodgson A.V., Hume J., Jackson A., Khan Z.M., Kovar-Smith C., Lewis L.R., Lozado R.J., Metzker M.L., Milosavljevic A., Miner G.R., Montgomery K.T., Morgan M.B., Nazareth L.V., Scott G., Sodergren E., Song X.-Z., Steffen D., Lovering R.C., Wheeler D.A., Worley K.C., Yuan Y., Zhang Z., Adams C.Q., Ansari-Lari M.A., Ayele M., Brown M.J., Chen G., Chen Z., Clerc-Blankenburg K.P., Davis C., Delgado O., Dinh H.H., Draper H., Gonzalez-Garay M.L., Havlak P., Jackson L.R., Jacob L.S., Kelly S.H., Li L., Li Z., Liu J., Liu W., Lu J., Maheshwari M., Nguyen B.-V., Okwuonu G.O., Pasternak S., Perez L.M., Plopper F.J.H., Santibanez J., Shen H., Tabor P.E., Verduzco D., Waldron L., Wang Q., Williams G.A., Zhang J., Zhou J., Allen C.C., Amin A.G., Anyalebechi V., Bailey M., Barbaria J.A., Bimage K.E., Bryant N.P., Burch P.E., Burkett C.E., Burrell K.L., Calderon E., Cardenas V., Carter K., Casias K., Cavazos I., Cavazos S.R., Ceasar H., Chacko J., Chan S.N., Chavez D., Christopoulos C., Chu J., Cockrell R., Cox C.D., Dang M., Dathorne S.R., David R., Davis C.M., Davy-Carroll L., Deshazo D.R., Donlin J.E., D'Souza L., Eaves K.A., Egan A., Emery-Cohen A.J., Escotto M., Flagg N., Forbes L.D., Gabisi A.M., Garza M., Hamilton C., Henderson N., Hernandez O., Hines S., Hogues M.E., Huang M., Idlebird D.G., Johnson R., Jolivet A., Jones S., Kagan R., King L.M., Leal B., Lebow H., Lee S., LeVan J.M., Lewis L.C., London P., Lorensuhewa L.M., Loulseged H., Lovett D.A., Lucier A., Lucier R.L., Ma J., Madu R.C., Mapua P., Martindale A.D., Martinez E., Massey E., Mawhiney S., Meador M.G., Mendez S., Mercado C., Mercado I.C., Merritt C.E., Miner Z.L., Minja E., Mitchell T., Mohabbat F., Mohabbat K., Montgomery B., Moore N., Morris S., Munidasa M., Ngo R.N., Nguyen N.B., Nickerson E., Nwaokelemeh O.O., Nwokenkwo S., Obregon M., Oguh M., Oragunye N., Oviedo R.J., Parish B.J., Parker D.N., Parrish J., Parks K.L., Paul H.A., Payton B.A., Perez A., Perrin W., Pickens A., Primus E.L., Pu L.-L., Puazo M., Quiles M.M., Quiroz J.B., Rabata D., Reeves K., Ruiz S.J., Shao H., Sisson I., Sonaike T., Sorelle R.P., Sutton A.E., Svatek A.F., Svetz L.A., Tamerisa K.S., Taylor T.R., Teague B., Thomas N., Thorn R.D., Trejos Z.Y., Trevino B.K., Ukegbu O.N., Urban J.B., Vasquez L.I., Vera V.A., Villasana D.M., Wang L., Ward-Moore S., Warren J.T., Wei X., White F., Williamson A.L., Wleczyk R., Wooden H.S., Wooden S.H., Yen J., Yoon L., Yoon V., Zorrilla S.E., Nelson D., Kucherlapati R., Weinstock G., Gibbs R.A.Nature 440:346-351(2006) Allelic variations of human keratins K4 and K5 provide polymorphic markers within the type II keratin gene cluster on chromosome 12.Wanner R., Foerster H.-H., Tilmans I., Mischke D.J. Invest. Dermatol. 100:735-741(1993) Molecular characterization and expression of the stratification-related cytokeratins 4 and 15.Leube R.E., Bader B.L., Bosch F.X., Zimbelmann R., Achtstaetter T., Franke W.W.J. Cell Biol. 106:1249-1261(1988) Wanner R., Tilmans I., Mischke D.Initial characterization of the human central proteome.Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J.BMC Syst. Biol. 5:17-17(2011) A glutamine insertion in the 1A alpha helical domain of the keratin 4 gene in a familial case of white sponge nevus.Terrinoni A., Candi E., Oddi S., Gobello T., Camaione D.B., Mazzanti C., Zambruno G., Knight R., Melino G.J. Invest. Dermatol. 114:388-391(2000) A novel mutation in the keratin 4 gene causing white sponge naevus.Chao S.C., Tsai Y.M., Yang M.H., Lee Jy J.Y.Br. J. Dermatol. 148:1125-1128(2003)

Other Notes

Small volumes of KRT4 recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for KRT4. It may not necessarily be applicable to this product.

NCBI GI #
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
NCBI Official Full Name
Keratin, type II cytoskeletal 4
UniProt Protein Name
Keratin, type II cytoskeletal 4
UniProt Synonym Protein Names
Cytokeratin-4; CK-4; Keratin-4; K4; Type-II keratin Kb4
Protein Family
UniProt Gene Name
KRT4
UniProt Synonym Gene Names
CYK4; CK-4; K4
UniProt Entry Name
K2C4_HUMAN

NCBI Summary for KRT4

The protein encoded by this gene is a member of the keratin gene family. The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. This type II cytokeratin is specifically expressed in differentiated layers of the mucosal and esophageal epithelia with family member KRT13. Mutations in these genes have been associated with White Sponge Nevus, characterized by oral, esophageal, and anal leukoplakia. The type II cytokeratins are clustered in a region of chromosome 12q12-q13. [provided by RefSeq, Jul 2008]
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UniProt Comments for KRT4

K4: Heterotetramer of two type I and two type II keratins. Keratin-4 is generally associated with keratin-13. Detected in the suprabasal layer of the stratified epithelium of the esophagus, exocervix, vagina, mouth and lingual mucosa, and in cells and cell clusters in the mucosa and serous gland ducts of the esophageal submucosa. Expressed widely in the exocervix and esophageal epithelium, with lowest levels detected in the basal cell layer. Belongs to the intermediate filament family.

Protein type: Cytoskeletal

Chromosomal Location of Human Ortholog: 12q13.13

Cellular Component: cell surface; intermediate filament; intermediate filament cytoskeleton; keratin filament; nucleus

Molecular Function: protein binding; structural molecule activity

Biological Process: cytoskeleton organization and biogenesis; epithelial cell differentiation; negative regulation of epithelial cell proliferation

Disease: White Sponge Nevus 1
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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