Azurocidin (AZU) ELISA Kit for Mouse

For Samples: Cell culture fluid & body fluid & tissue homogenate Serum or blood plasma

Intended Uses: This AZU ELISA kit is intended for laboratory research use only. The stop solution changes the color from blue to yellow and the intensity of the color is measured at 450 nm using a spectrophotometer. In order to measure the concentration of AZU in the sample, this AZU ELISA Kit includes a set of calibration standards. The calibration standards are assayed at the same time as the samples and allow the operator to produce a standard curve of Optical Density versus AZU concentration. The concentration of in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Principle of the Assay: The coated well immunoenzymatic assay for the quantitative measurement of AZU utilizes a multiclonal anti-AZU antibody and an AZU-HRP conjugate. The assay sample and buffer are incubated together with AZU-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the AZU concentration since AZU from samples and AZU-HRP conjugate compete for the anti-AZU antibody binding site. Since the number of sites is limited, as more sites are occupied by AZU from the sample, fewer sites are left to bind AZU-HRP conjugate. Standards of known AZU concentrations are run concurrently with the samples being assayed and a standard curve is plotted relating the intensity of the color (Optical Density) to the concentration of AZU. The AZU concentration in each sample is interpolated from this standard curve.

Small volumes of AZU elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS720075 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the azurocidin, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing AZU. The ELISA analytical biochemical technique of the MBS720075 kit is based on AZU antibody-AZU antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect AZU antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, AZU. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).