Anti-Proteinase 3 Antibody IgG (PR3 ab-IgG) ELISA Kit for Mouse

Principle of the Assay: PRO3-Ab IgG ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-PRO3-Ab IgG antibody and an PRO3-Ab IgG-HRP conjugate. The assay sample and buffer are incubated together with PRO3-Ab IgG-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the PRO3-Ab IgG concentration since PRO3-Ab IgG from samples and PRO3-Ab IgG-HRP conjugate compete for the anti-PRO3-Ab IgG antibody binding site. Since the number of sites is limited, as more sites are occupied by PRO3-Ab IgG from the sample, fewer sites are left to bind PRO3-Ab IgG-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The PRO3-Ab IgG concentration in each sample is interpolated from this standard curve.

Intended Uses: This PRO3-Ab IgG ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse PRO3-Ab IgG. This ELISA kit for research use only, not for therapeutic or test applications!

Small volumes of PR3 ab-IgG elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS721278 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Anti-proteinase 3 antibody IgG (PR3 ab-IgG) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing PR3 ab-IgG. The ELISA analytical biochemical technique of the MBS721278 kit is based on PR3 ab-IgG antibody-PR3 ab-IgG antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect PR3 ab-IgG antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, PR3 ab-IgG. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).